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자연발생한 돼지 다발성 장막염 예로부터 Haemophilus parasuis의 분리와 면역조직화학적 진단
배유찬,강문일,황의경,손현주,최상호,Bae, You-chan,Kang, Mun-il,Hwang, Eui-kyong,Sohn, Hyun-joo,Choi, Sang-ho 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.4
From Jan. 1996 to Oct. 1997, 29 pigs with 40-70 days old showing dyspnea inappetite and polyserositis were collected and carried out necropsy, bacterial culture, histopathology, avidin biotin complex(ABC) stain, fluorescent antibody(FA) test, and electron microscopy. In the study, 4 strains from 3 pigs were isolated from meninges, pleura and synovial fluid and also were identified as Haemophilus parasuis serovar 5. Main histopathological lesions of 29 pigs with polyserositis were frequently composed of fibrinous peritonitis(27), pleurisy(22), interstitial pneumonia(21), fibrinous epicarditis(20), fibrinopurulent meningitis(8) and synovitis(4). By ABC stain, 11/29(37.9%) pigs with polyserositis were confirmed to have H parasuis serovar 5 in the cytoplasm of macrophages and neutrophils in cerebral meninges, epicardium, pleura surface of lung or serosa of spleen. ABC stain(20.8~40.0%) to detect H parasuis serovar 5 in tissues was more sensitive than bacterial culture(10.3%), but less sensitive than FA test(62.5%) using frozen tissues even though the result of 8 cases. By electron microscopy, a bacterium was also detected in the cytoplasm of macrophages in purulent exudate of cerebral meninges. Consequently, we confirmed that H parasuis serovar 5 has been involving to cause pigs with polyserositis and can be detected by FA and ABC stain as reliable diagnostic tools.
2008년 국내 고병원 조류인플루엔자 발생 관련 의사환축 등 시료에 대한 검사결과
배유찬,우계형,김하영,박중원,이종형,주이석,Bae, Yu-Chan,U, Gye-Hyeong,Kim, Ha-Yeong,Park, Jung-Won,Lee, Jong-Hyeong,Ju, Lee-Seok 대한수의사회 2008 대한수의사회지 Vol.44 No.11
2008년 고병원성 조류인플루엔자의 발생으로 인해 양축 농가, 방역기관 관계자를 포함한 많은 국민들이 큰 시련을 겪었다. 다행히 이 질병이 초기에 근절되어 우리나라는 고병원성 조류인플루엔자 청정국임을 선포하게 되었다. 이 질병의 조기 근절에는 신속 정확한 진단이 기반이 되어 적절한 방역 대책이 수행되었기 때문으로 생각한다. 본고에서는 금년 고병원성 조류인플루엔자 발생과 관련되어 국립수의과학검역원 질병진단센터에서 수행한 의사환축 신고건, 병성감정 의뢰건, 역학조사 관련 시료 등에 대한 정밀검사 결과를 보고하고자 한다.
국내 발생 돼지 구제역의 임상증상, 육안병변 및 현미경적 병변
배유찬,윤순식,강경일,노인순,김희진,소병재,박중원,진영화,강문일 한국임상수의학회 2004 한국임상수의학회지 Vol.21 No.2
We described the clinical signs, gross lesions, histopathological lesions, and electronmicroscopy of pig Foot-and- Mouth Disease cases which had occurred in 2002, Korea. Grossly affected pigs showed vesicles on snout, tongue, coronary band, and udder. Histoapthologically, severe intercellular edema and necrosis of prickle cells, and separation between epidermis and dermis were found on the mucosa of tongue and coronary band. And myocardial necrosis and mononuclear cells infiltration in myocardium were found. Electronmicroscopically numerous picornavirus particles(18~22 nm in diameter) were found in the cytoplasm of prickle cells. By those results, we confirm that those cases were typical FMD cases.