2D PIC 시뮬레이션을 이용한 마그네트론 스퍼터링 증착 공정의 이온 특성 변화 분석 및 증착 모델링

박희성,오세훈,이해준 한국진공학회 2018 한국진공학회 학술발표회초록집 Vol.2018 No.8

Fungal Elicitor와 기계적 상해에 의한 토마토 HMGR mRNA의 증가

박희성,이용세 한국식물병리학회 1996 Plant Pathology Journal Vol.12 No.3

재래산양에 있어서 핵이식란의 융합조건이 융합 및 체외발달에 미치는 영향

박희성,김태숙,이윤희,정수영,이명열,홍승표,박준규,김충희,정장용 사단법인 한국동물생명공학회 2004 Reproductive & developmental biology Vol.28 No.2

재래산양의 과배란처리에 있어서 회수시간이 난자의 회수율에 미치는 영향

박희성,홍승표,김선훈,정수영,진종인,김택석,이지삼 한국동물번식학회 2003 Reproductive & Developmental Biology(Supplement) Vol.27 No.1s

재래산양때 있어서 난포란의 활성화 처리방법이 체외발달에 미치는 영향

박희성,이명열,김현정,권병철,이윤희,박준규,정장용 한국동물번식학회 2003 Reproductive & Developmental Biology(Supplement) Vol.27 No.1s

Assessment of the Impact of Anti-Hormonal Treatment on Bone Health in Patients With Breast Cancer Using Machine-Learning Analysis

박희성,박미영,김근영,강태우 한국유방암학회 2023 Journal of Breast Disease Vol.11 No.1

Purpose: This study analyzed the effects of anti-hormonal treatment (HTx) on bone health using real-world evidence and machine-learning analysis.Methods: We extracted 20 clinical variables and patient history of HTx by reviewing the records of 244 patients treated for breast cancer between January 2014 and June 2018 at Pusan National University Hospital. Baseline and first follow-up dual-energy absorptiometry were analyzed. To identify which of the 20 clinical variables were highly associated with the patients’ bone mineral density and trabecular bone score (TBS), we applied partial least squares discriminant analysis (PLS-DA) and MetaboAnalyst. A self-organizing map (SOM) was used to sort the patient groups based on the selected variables.Results: The patients were classified as ‘no change’ (n=161, 70.6%), ‘deteriorated’ (n=43, 18.9%), or ‘improved’ (n=24, 10.5%) according to the change in TBS during the follow-up period. The baseline TBS value was significantly lower in the improved group. The top five variables (age, HTx, duration of vitamin D and/or calcium intake, cancer stage, and body mass index) were selected using PLS-DA, which generated variable importance value (VIP) scores for all variables and high VIP scores contributed greatly to patient classification. To identify the patients’ clinical patterns using the top five selected variables, a 3×4 grid structure SOM was generated. Clusters were selected to represent the most improved, no change, and most deteriorated groups.Conclusion: This study evaluated the clinical association between HTx and bone health in patients with breast cancer under various clinical conditions and found that the characteristics of patients included in the study were too heterogeneous to be classified in clusters. Therefore, additional data should be collected for future research.

재래산양의 체세포 핵이식에 의한 복제수정란의 체외발달에 관한 연구

박희성,김태숙,정수영,이윤희,정장용 韓國受精卵移植學會 2005 한국동물생명공학회지 Vol.20 No.2

본 연구는 재래산양에서 복제 수정란의 생산효율을 향상시키기 위한 기초 자료를 제시하고자 체세포 핵이식을 실시하여 공핵세포의 종류, 핵이식란의 활성화 처리 방법 및 수핵난자의 조건이 체외발달율에 미치는 영향을 조사, 검토하여 핵이식란 생산을 위한 최적의 조건을 규명하고자 실시하였다. 공핵세포의 종류에 따른 핵이식란의 체외발달율은 융합이 이루어진 핵이식란의 활성화 처리 후 분할율은 귀 유래 섬유아세포를 공핵세포로 사용하였을 때가 로서 태아 유래 섬유아세포를 The present study was conducted to examine some factors affecting in vitro development of oocytes from somatic cell nuclear transfer (SCNT) in Korean native goats. Recipient oocytes were surgically collected after superovulation by using CIDR and FSH, PMSG, hCG and estrous synchronization in Korean Native goats. For nuclear transfer, the fibroblasts from caprine ear cells and fetal fibroblasts were surgically harvested and were cultured in vitro until cell confluency in serum-starvation condition (TCM-199 + FBS) for 3 to 5 days. The zona pellucidae of matured oocytes were partially drilled by laser irradiation. A single somatic cell was individually transferred into each enucleated oocyte. The reconstructed oocytes were then electrically fused and activated. Activated NT embryos were cultured in mSOF medium supplemented with in air. There were no significant difference in the number of embryos cleaved and 4-cell development between the fibroblast nuclei from mature ear cells and fetal cells, but the rate of 8-cell development was higher (P<0.05) in ear cells than in fetal cells . However, the embryo development to morula or blastocyst was not significantly different between both the groups, respectively. The number of embryo cleaved were higher (P<0.05) in the oocytes activated with ionomycin+6-DMAP than in the oocytes activated electrically . The development of fused embryos to morula or blastocyst was found in ionomycin+6-DMAP, but no morula or blastocysts were developed in electrical stimulation. The development rate of SCNT embryos to morula or blastocyst was love. (P<0.05) in SCNT embryos than that in parthenotes . In the parthenotes, the cleavage rate and development to morula or blastocyst were significantly higher (P<0.05) as in ovulated oocytes than in follicular oocytes , respectively. These results suggest that some factors Including superovulation treatment, oocyte source, maturation of follicular oocytes, activation method and culture condition may affect in vitro developmental capability of embryos produced by somatic cell nuclear transfer in Korean Native goats, and the fusion rate be greatly low compared with other species.

생쥐의 배란유기 및 수정란이식

박희성,박준규,정장용 진주산업대학교 1991 論文集 Vol.29 No.-

본 연구는 최적의 수정란 이식기법을 개발 확립하고자 ICR계통의 생쥐에 배란유기와 포배기에 있는 수정란을 난관이식, 자궁이식, 외과적 및 비외과적 이식을 실시하여 이들 이식방법에 따른 산자생산 성적을 조사한 결과는 다음과 같다. 1. 자연발정 및 과배란유기시 분만한 어미당 평균산자수는 각각 11.7 및 13.8마리로서 과배란을 유기시켰을때가 산자수가 다소 적었으나 이들간에 유의적(P<0.05)인 차이는 없었다. 2. 자연발정 및 과배란을 유기한 수란생쥐에 포배기 수정란을 이식하였을 경우 수란생쥐의 평균수태율은 각각 27.7 및 10.2마리로써 자연발정이 유기된 수란생쥐가 과배란을 유기한 수란생쥐보다 평균 수태율이 다소 높았다 (P<0.05). 3. 수정란이 이식부위에 따른 수태율은 자궁이식의 경우 48.9%로써 난관이식시의 39.5%보다 다소 높았다(P<0.05) 4. 자연발정이 유기된 수란생쥐에 위임신을 유기하여 상실배기에 잇는 수정란을 외과적이식의 경우 수태율은 34.4%로써 비외과적 이식의 경우 21.5%보다 유의적(P<0.05)으로 산자생산율이 높았다. 5. 자연발정이 유기된 수란생쥐에 위임신을 유기하여 채란직후의 수정란을 이식하였을 때의 수태율은 35.4%로써 2-세포기 수정란을 포배기까지 체외 배양후 이식을 실시하였을 경우 32.1%의 수태율을 얻어 이들간에 유의적(P<0.05)인 차이는 없었다. These experiments were carried out to determine the effects ovulation methods in pseudpregnant recipient on the subsequent transfer site and transfer methods of embryo in mouse. The embryos of ICR mouse collected 2-cell and blastocyst stages. These embryos were oviduct or uterous and surgical or non-surgical transfer to pseoudopregnant recipient mice, the results obtained from this study were summarized as follows : 1. Production of live young from pregnant female mouse at superovulation 11.7 head was significantly(P<0.05) lower than this at 13.8 head natural ovulation. 2. The conception rate of mice embryo transfer was 27.7% from natural ovulation, and lower 10.2% from superovulation at blastocyst embryos transferred in recipients(P<0.05) 3. The conception rate of mice embryo transfer was 48.9% from uterous transfer, and lower 39.5% from oviduct transfer at blastocyst embryos transfer site of recipients(P<0.05) 4. When compared with the result of conception rate(21.5%) after non-surgical transfer of blastocysts, significantly(P<0.05) higher results were obtained with surgical transfer(34.4%). 5. When compared with the result of conception rate(35.4%) after fresh embryo transfer of blastocysts, similer(P<0.05) results were obtained with cultured embryo transfer(32.1%)

저궤도 위성을 위한 전력계 검증 장치 개발

박희성,박성우,장성수,장진백,이종인 한국우주과학회 2006 한국우주과학회보 Vol.15 No.2

생쥐 수정란의 핵이식후 체외발달에 관한 연구

박희성,이효종,최상용,박충생 한국동물번식학회 1990 Reproductive & developmental biology Vol.14 No.3

Single nuclei from two-, four- and eight-cell mouse embryos were transplanted into enucleated two-cell mouse embryos by micromanipulation and sendai virum mediated fusion. no significant difference in successful injectin rate and fusion rate was found between the cell stages of nuclear donor embryos. There nuclear transplant embryos receiving different cell stage nuclei were cultured in vitro for 96 hours. 75.3% of 255 embryos receiving 2-cell nuclei, 68.2% of 236 embryos reciving 4-cell nuceli and 46.9% of 228 embryos receiving 8-cell nuclei were developed to blastocyst, respectively. The number of blastomeres was significantly(P<0.05) reduced in the embryos receiving 8-cell nuclei, compared with the embryos receiving 2-cell, 4-cell nuclei or the intact embryos. Also the size of blastocysts was significantly(P<0.05) smaller in the embryos receiving 8-cell nuclei, compared with the intact or other nuclear transplant embryos. These results suggest that single nuclei introduced into the enucleated two-cell embryos are able to support the in vitro development of the reconstituted embryos to blastocysts. The prominant retardation of blastocoele formation and cell division was shown in nuclear transplant embryos receiving eight-cell nuclei when they were cultured in vitro.