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민영일(Young Il Min),이성구(Sung Koo Lee),김명환(Myung Hwan Kim),이승규(Sung Gyu Lee),이선영(Sun Young Yi),홍진선(Jin Sun Hong),박흥동(Hung Dong Park),공경엽(Kyung Youp Kong),김미경(Mee Kyung Kim) 대한소화기학회 1993 대한소화기학회지 Vol.25 No.2
Primary sclerosing cholangitis is a chronic disease of unknown cause, characterized by inflammation and fibrosis of the biliary tree with diffuse multifocal stricture formation. In our case, the cholangiogram showed multifocal strictures in the intrahepatic ducts and beading of extrahepatic bile ducts. Also severe periductal fibrosis and proliferation of bile ductules were noted by liver biopsy. Authors eport one case of primary sclerosing cholangitis in 27-year-old rnale who complained right upper quadrant abdominal pain and pruritus.
내시경적 비담도 배액법 ( Endoscopic Nasobiliary Drainage ) 으로 치유된 담즙유출
민영일(Young Il Min),이성구(Sung Koo Lee),김명환(Myung Hwan Kim),이선영(Sun Young Yi),홍진선(Jin Sun Hong),박흥동(Hung Dong Park) 대한소화기학회 1993 대한소화기학회지 Vol.25 No.4
A 34 year old man was referred after primary suture of the liver and cholecystectomy due to liver rupture following blunt abdominal trauma. He was diagnosed as having an injury of the intrahepatic bile leak. The setting was considered as an indication for endoscopic nasobiliary drainage, a nasobiliary tube was inserted and kept so during his hospitalization. After insertion of nasobiliary tube, his general condition gradually improved and follow-up image studies obtained 2 weeks later revealed good bile passage into duodenum without bile leakage. Accordingly the nasobiliary tube was removed. In conclusion, endoscopic nasobiliary drainage will be a good alternative treatment to surgical or percutaneous drainage in the bile leak related bile duct injury.
역전사 중합효소 연쇄반응 ( RT-PCR ) 에 의한 HCV-RNA 의 검출 Biotin 및 방사성옥소 표지 Primer 로 구성된 Kit 의 이용
문대혁(Dae Hyuk Moon),류진숙(Jin Sook Ryu),이영상(Young Sang Lee),정영화(Young Hwa Chung),천준홍(Jun Hong Cheon),정윤영(Yoon Young Chung),박흥동(Hung Dong Park) 대한핵의학회 1994 핵의학 분자영상 Vol.28 No.2
N/A This study was performed to evaiuate the clinical applicability of the reverse t.ranscription polymerase chain reaction (RT-PCR) kit of HCV-RNA using biotinylated and radioiodinat.ed primers. Study subjects were 118 patients with positive anti-HCV. HCV-RNA in patient.s serurn was ext.racted by guanidium thiocyanate method. After first amplification, the product. was reamplified by primers labelled with biotin and I-125. The final amplification product was de- tected by counting t,he radioactivity after incubation in avidin coated tubes. In 51 samples, t.he test was repeated for evaluation of reproducibility. This new method was also compared with conventional RT-PCR rnethods in 34 samples from patients with chronic liver disease. The results were as follows, 1) HCV-RNA was positive in 85(97%)of 88 patients with chronic liver disease, and in 23 (73%) of 30 pat.ients with normal liver function. 2) In comparison with conventional method, HCV-RNA was detected in 32(94%) of 34 patient.s with new method, whereas in 27(79% ) of the same group with conventional method, 3) Repeated test with new rnet.hod in 52 samples demonstrat.ed 82% of concordant result. In conclusion, new rnethod with biotinylated and radioiodinated primers was rnore sensitive than conventional method. However, great care must be taken for quality control because there were considerable interassay variat,ion and possiblity of false positivity and false negativity.