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남해안 패류양식장에서 분리된 대장균(Escherichia coli)의 항균제 내성
박큰바위 ( Kunbawui Park ),박준용 ( Jun Yong Park ),조미라 ( Mi Ra Jo ),이희정 ( Hee Jung Lee ),김지회 ( Ji Hoe Kim ),오은경 ( Eun Gyong Oh ),신순범 ( Soon Bum Shin ),김연계 ( Yeon Kye Kim ),이태식 ( Tae Seek Lee ) 한국수산과학회 2013 한국수산과학회지 Vol.46 No.5
This study evaluated the impact of antimicrobial resistant bacteria produced by inland pollution sources on coastal areas, and investigates antimicrobial resistance profiles of bacteria isolated from marine filter feeders. A total of 107shellfish (short-necked clams Ruditapes philippinarum, ark shells Scapharca broughtonii, and oysters Crassostrea gigas) were collected from the southern coast of Korea, from which 204 Escherichia coli strains were isolated. The antimicrobial susceptibility patterns of isolated strains were analyzed for 15 antimicrobial agents used in Korea for clinical or veterinary therapy. Antimicrobial resistance was present in 44.6% of E. coli isolates against at least one antimicrobial agent. The rate of antimicrobial resistance in the Narodo area was higher than isolates from the Gangjinman area and Kamak Bay. E. coli isolates had a higher rate of resistance against: tetracycline (29.9%), streptomycin (25.5%), and trimethoprim (14.2%). Of 204 isolates, 29 (14.2%) were resistant to multiple antimicrobial agents.
한산거제만 및 자란만사량도 해역 육상오염원 배출수에서 분리한 대장균의 항균제 내성 패턴
박큰바위 ( Kunbawui Park ),김송희 ( Song Hee Kim ),함인태 ( In Tae Ham ),류아라 ( A Ra Ryu ),권지영 ( Ji Young Kwon ),김지회 ( Ji Hoe Kim ),유홍식 ( Hong Sik Yu ),이희정 ( Hee Jung Lee ),목종수 ( Jong Soo Mok ) 한국수산과학회(구 한국수산학회) 2018 한국수산과학회지 Vol.51 No.1
We investigated patterns of antimicrobial resistance in Escherichia coli isolated from the water discharged from inland pollution sources in the Hansan-Geojeman and Jaranman-Saryangdo areas of Korea. A total of 217 strains of E. coli were isolated from 23 point-sources. These strains were tested for their susceptibility to 16 antimicrobial agents used in Korea for medical or veterinary therapy. The highest level of antibiotic resistance among the isolated strains was to tetracycline 10.6%, followed by ampicillin (3.2%), nalidixic acid (2.8%), rifampin (1.8%), trimethoprim (1.8%), trimethoprim/sulfamethoxazole (1.8%), chloramphenicol (1.4%), streptomycin (1.4%), cephalothin (0.5%) and gentamicin (0.5%). Resistance to at least one antimicrobial agent was present in 17.1% of the E. coli isolates. Only four of the isolated strains of E. coli showed multiple antibiotic resistance, which is defined as resistance to more than four antibiotics.
강우 발생에 따른 육상오염원이 나로도 해역의 세균학적 수질에 미치는 영향 평가
박큰바위 ( Kunbawui Park ),조미라 ( Mi Ra Jo ),김연계 ( Yeon Kye Kim ),이희정 ( Hee Jung Lee ),권지영 ( Ji Young Kwon ),손광태 ( Kwang Tae Son ),이태식 ( Tae Seek Lee ) 한국수산과학회(구 한국수산학회) 2012 한국수산과학회지 Vol.45 No.5
This study evaluated the effect of the inland pollution sources on the bacteriological water quality in Narodo area after rainfall events. Following 16 mm of rainfall, the water discharged from Dohwa Stream and contaminants from Balpo village had a very limited effect on bacteriological water quality in adjacent area and the affected area did not extend to the designated area. In comparion, after 33 mm of rainfall, the density of fecal coliforms at stations located in the mouth of Dohwa stream and the discharg point of contamination sources located in Balpo village were lower than after 16 mm of rainfall. These results were obtained during an extreme high tide near the coast the contaminants discharged from Dohwa stream and Balpo village. Therefore, it is necessary to conduct a detailed survey to examine the dffects of tides on the diffusion characteristics of the contaminants discharged from Dohwa stream and Balpo village in order to manage of Narodo area efficiently.
굴(Crassostrea gigas), 멍게(Halocynthia roretzi) 및 해삼(Apostichopus japonicus)에서 분리한 대장균의 항균제 내성 특성
박큰바위,류아라,김송희,함인태,권지영,김지회,유홍식,이희정,목종수,Park, Kunbawui,Ryu, A Ra,Kim, Song Hee,Ham, In Tae,Kwon, Ji Young,Kim, Ji Hoe,Yu, Hong Sik,Lee, Hee Jung,Mok, Jong Soo 한국수산과학회 2017 한국수산과학회지 Vol.50 No.5
This study evaluated the abundance of fecal coliforms in oysters Crassostrea gigas, sea squirts Halocynthia roretzi and sea cucumbers Apostichopus japonicus in fisheries along the coast of Korea in 2014, and investigated the prevalence of antimicrobial resistance in Escherichia coli isolated from these fishery products. The ranges of fecal coliforms found in oysters, sea squirts and sea cucumbers were <18-20, <18-330 and <18-3,300 MPN (most probable number)/100 g, respectively. Sea squirts contained the greatest range of E. coli (<20-140 MPN/100 g), followed by sea cucumbers (<20-130 MPN/100 g) and oysters (<20-20 MPN/100 g). A total of 26 strains of E. coli were isolated from 34 sea squirt, 25 sea cucumber and 13 oyster samples. Strains thus isolated were tested for their susceptibility to 22 antimicrobial agents used in Korea for medical or veterinary therapy. E. coli isolates showed the greatest resistance to ampicillin (84.6%), followed by trimethoprim (34.6%), nalidixic acid (34.6%), tetracycline (30.8%), pipemidic acid (26.9%), streptomycin (23.1%), chloramphenicol (23.1%), trimethoprim/sulfamethoxazole (23.1%), and gentamicin (15.4%). Resistance to at least one antimicrobial agent was present in 88.5% of E. coli isolates. Of the 26 isolated, six strains (23.1%) were resistant to multiple antimicrobial agents.
남해안 패류양식장에서 분리한 Vibrio parahaemolyticus의 병원인자분포 및 항균제내성
박용수 ( Yong Soo Park ),박큰바위 ( Kunbawui Park ),권지영 ( Ji Young Kwon ),유홍식 ( Hong Sik Yu ),이희정 ( Hee Jung Lee ),김지회 ( Ji Hoe Kim ),이태식 ( Tae Seek Lee ),김풍호 ( Poong Ho Kim ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.4
From 2013 through 2015, we investigated the contamination status and antimicrobial resistance patterns of pathogenic Vibrio parahaemolyticus in commercially valuable seawater and shellfish (Oyster Crassostrea gigas, short-neck clam Venerupis philippinarum, ark shell Scapharca broughtonii and mussel Mytilus galloprovinciallis) from the southern coast of Korea. The detection rate of V. parahaemolyticus was highest in short-neck clams (23.7%), followed by ark shells (19.2%), oysters (15.9%), mussels (13.6%), and seawater (8.6%). The following percentages of PCR assays of shellfish were positive for the thermostable direct hemolysin-related hemolysin gene (trh) : oysters (12.8%), shortneck clams(11.8%), and ark shells (3.4%). Similar assays for the thermostable direct hemolysin gene (tdh) resulted in positive results for short-neck clams (5.9%) and ark shells (3.4%). Antimicrobial resistance was present in 100% of 8 tdh (+) and 2 trh (+) V. parahaemolyticus isolates challenged with ampicillin. However, all pathogenic V. parahaemolyticus were sensitive to 14 other antibiotics. To ensure the safety of shellfish consumption, the continuous monitoring of the prevalence and distribution of virulence factors of V. parahaemolyticus in shellfish farms is needed.
권지영 ( Ji Young Kwon ),박큰바위 ( Kunbawui Park ),송기철 ( Ki Cheol Song ),오은경 ( Eun Gyoung Oh ),이희정 ( Hee Jung Lee ),조미라,김지회 ( Ji Hoe Kim ),손광태 ( Kwang Tae Son ) 한국수산과학회(구 한국수산학회) 2012 한국수산과학회지 Vol.45 No.5
From 1998 to 2008, the bacteriological quality of seawater in Kamak flay, Korea, was monitored. The total and fecal coliforms in seawater were monitored as bacterial indicators, and the level of fecal coliform contamination was used to evaluate seawater quality. In the southern shellfish growing area of Karnak Bay, the observed feeal coliform geometric mean (GM) and estimated 90th percentile did not exceed the quality standards for fecal coliforms in seawater in Korea and th USA (GM<l4 MPN/l00mL, 90th percentile<43 MPN/I00rnL). The GM and estimated 90th percentile of the fecal coliform levels in the adjacent northern area of Kamak Bay were higher than in the southern area. Stations near wastewater discharge sites and urban areas in the north had the lowest quality and did not meet the bacteriological seawater quality criteria. The high fecal coliform levels in the Seonso coastal region near wastewater discharges and northern urban areas declined significantly following dredging and operation of a sewage treatment plant.
경남 연안 해만 가리비(Argopecten irradians)의 부위별 마비성 패류독소 분포
김동욱 ( Dong Wook Kim ),박큰바위 ( Kunbawui Park ),하광수 ( Kwang Soo Ha ),류아라 ( Ara Ryu ),유헌재 ( Hean Jae Yu ),조성해 ( Seong Hae Jo ),조성래 ( Sung Rae Jo ),목종수 ( Jong Soo Mok ) 한국수산과학회(구 한국수산학회) 2019 한국수산과학회지 Vol.52 No.3
To understand the characteristics of paralytic shellfish poisoning in a major production area of the bay scallop Argopecten irradians in Korea, the seasonal variation and anatomical distribution of paralytic shellfish toxin (PST) were determined in bay scallops collected from the Gyeongnam coast of Korea from March to May 2018. PST levels in bay scallops in the survey area showed remarkable seasonal variation. PST was first detected at a level of 0.42 mg/kg on April 2, 2018, and the highest toxin level (3.15 mg/kg) was recorded on April 12. Among the tissues of bay scallops, the highest proportion of PST was found in the viscera (54.9%±17.8%), followed by the adductor (22.8%±10.9%), gonads (8.9%±4.6%), gills (7.1%±3.7%), and mantle (6.3%±.8%). In addition, with higher PST levels in the whole tissues of bay scallops, the proportion of PST in the viscera increased, whereas those in the mantle, gill, and gonad tissues decreased. In a high-toxicity group with more than 2.0 mg/kg PST in the whole tissues, the proportion of PST in the viscera was 71.8%±6.7%.
심길보 ( Kil Bo Shim ),박큰바위 ( Kunbawui Park ),윤나영 ( Na-young Yoon ),안병규 ( Byoung Kyu An ),인정진 ( Jung Jin In ),한형구 ( Hyeong Gu Han ),이우진 ( Woo Jin Lee ) 한국수산과학회 2021 한국수산과학회지 Vol.54 No.6
Eighty-nine different types of commercially salt-fermented fishery products comprising various raw materials were analyzed for total aerobic bacteria, number of coliform bacteria, fecal coliform, and Escherichia coli. The food-poisoning bacterial content of the samples was investigated using next-generation sequencing. The mean mass of total aerobic bacteria in Jeotgal was 6-1.8×10<sup>9</sup> CFU/g, and that in Aekjeot and Sikhae was 4-2.2×10<sup>5</sup> CFU/mL and 1.9×105-8.4×10<sup>8</sup> CFU/g, respectively. Coliform bacteria were detected in 9 (28.1%) of 32 Jeotgal samples; 15 (46.8%) of 32 seasonal Jeotgal samples; and in 5 (55.5%) of 9 Sikhae samples. Fecal coliform and E. coli were not detected in 86 of the 89 samples. Yersinia enterocolitica was detected only in Galchi jeot (salt-fermented hairtail) (1 type) and not in other Jeotgal samples. These results contribute to our knowledge regarding the bacterial stability of salt-fermented fishery products.
해수 중의 수온, 염분 및 pH에 따른 노로바이러스 및 Male Specific Coliphage 농도변화
김풍호 ( Poong Ho Kim ),박용수 ( Yong Soo Park ),박큰바위 ( Kunbawui Park ),권지영 ( Ji Young Kwon ),유홍식 ( Hong Sik Yu ),이희정 ( Hee Jung Lee ),김지회 ( Ji Hoe Kim ),이태식 ( Tae Seek Lee ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.4
Pre- or post-harvest processing is required to mitigate the risk of norovirus infection mediated by shellfish or seafood. We investigated the environmental resistance of human norovirus (HuNoV) under various conditions of temperature, salinity, and pH in seawater. Male-specific coliphage (MSC) was as the reference virus for all tests. At 4℃, HuNoV GII4 spiked into seawater was continually detected by RT-PCR for 35 days, regardless of salinity or pH level. It maintained nearly stable concentrations, meaning HuNoV can sustain a viral population in seawater long enough to be accumulated by shellfish and other filter feeders during winter. MSC was also stable at 4℃ although viral infectivity dropped sharply after 28 days. The effects of salinity and pH on MSC were indistinct. At 25℃ the detectable period of HuNoV GII4 by RT-PCR in seawater decreased to about one-third or half of the period at 4℃. High salinity (32 psu) and alkaline pH (8.5) were also unfavorable for sustaining HuNoV abundance at 25℃ in seawater. The resistance patterns of MSC to high temperature, high salinity, and alkaline pH were more dramatic and viral infectivity decreased over time, almost in direct proportion to experimental days. MSC was undetectable after 12 days under all salinities and pH levels at 25℃.
자연정화에 의한 양식굴(Crassostrea gigas) 중 노로바이러스 저감화
유홍식 ( Hongsik Yu ),박용수 ( Yong Su Park ),안세라 ( Sera An ),박큰바위 ( Kunbawui Park ),심길보 ( Kil Bo Shim ),송기철 ( Ki Cheol Song ),이태식 ( Tae Seek Lee ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.2
The efficacy of depuration following growing area translocation for the defecation of norovirus was evaluated under experimental conditions using oysters Crassostrea gigas previously subjected to bioaccumulation of this virus at a waste treatment plant discharge site. Three trials were assayed in an open experimental system with a commercial oyster farm located in a shellfish growing area approved by the Korean Shellfish Sanitation Program. Real-time reverse-transcription polymerase chain reaction (RT-PCR) was used to quantify viruses in the digestive glands of oysters. The final viral loads in oysters after 12 days remained under the detection limit (10 copies/g digestive gland) of the real-time RT-PCR. This reduction trend showed two-phase removal kinetics, with an initial slow reduction or slight increase in viruses during the first 2 days of depuration and subsequent stabilization with 0.12 to 2.64 log unit norovirus copies/g digestive gland per 2 days of depuration for the remaining time.