http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
박철범,성제경,이인세,이흥식,이준섭,윤여성,Park, Cheol-beom,Seong, Je-kyung,Lee, In-se,Lee, Heungshik S.,Lee, Joon-sup,Yoon, Yeo-sung 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.3
This study was performed to investigate the gross anatomical features and microscopical structures of the hemal nodes and the hemolymph nodes in the sheep. The hemal nodes and hemolymph nodes were observed mainly in periphery of the thoracic and abdominal aortae of the animals. The size of hemal nodes was generally smaller than that of the hemolymph nodes, and the shape of the both organs was spherical or ovoid. The color of the hemal nodes was red, while that of the hemolymph nodes was grary with red bands. The hemal nodes were surrounded by a relatively thick connective tissue capsule and there were extensive subcapsular sinuses distended by a great number of erythrocytes. Although a few number of lymphatic nodules and small areas of diffuse lymphatic tissues were observed in the parenchyma, no typical cortex and medulla were defined in the hemal node. Small numbers of blood vessels were found at the connective tissue capsule but lymph vessel was not observed microscopically in this organ. The hemolymph nodes were covered by a relatively thick connective tissue capsule and there was a hilus in each node. The parenchyma was divided into cortex and medulla. The cortex was composed of a few numbers of lymphatic nodules and some diffuse lymphatic tissues. The medulla comprised medullary sinuses and cords. Afferent and efferent lymph vessels were observed at the periphery of the capsule adn the hilus, respectively. The subcapsular and medullary sinuses were not extensive but filled with small numbers of erythrocytes. The stroma of hemal node and hemolymph node was composed of reticular cells and fibers, and the capsule and trabecula consisted of collagenous fibers with smooth muscle fibers.
박철범,성제경,이인세,이흥식,이준섭,윤여성,Park, Cheol-beom,Seong, Je-kyung,Lee, In-se,Lee, Heungshik S.,Lee, Joon-sup,Yoon, Yeo-sung 대한수의학회 2000 大韓獸醫學會誌 Vol.40 No.4
It has been known that the hemolymph node is one of the lymphoid organs found normally in ruminants and some rodents, and this organ shares morphological and functional characteristics of lymph node and spleen. To clarify the rigorous definition of morphological structures and functions of the hemolymph node in Sprague-Dawley(SD) rats, we examined these organs of SD rats gross anatomically and light microscopically. The hemolymph nodes were normally found in the abdominal cavity and in the neck of SD rats. This organ was surrounded by a thin connective tissue capsule and there was a hilus. The parenchyma comprised a cortex of lymphatic nodules and diffuse lymphatic tissues, and a medulla of diffuse lymphatic tissues arranged in cords. Afferent and efferent lymph vessels were observed but there was no extensive subcapsular and medullary sinuses. These sinuses were filled with erythrocytes. The stroma of hemolymph nodes was composed of reticular cells and fibers, and many lymphocytes, granulocytes, erythrocytes, plasma cells, macrophages and megakaryocytes were supported by the reticular network. The above findings suggest that the hemolymph nodes of SD rats may take part in blood formation, blood filtration and immune reaction.
박철범,성제경,이인세,이흥식,이준섭,윤여성,Park, Cheol-beom,Seong, Je-kyung,Lee, In-se,Lee, Heungshik S.,Lee, Joon-sup,Yoon, Yeo-sung 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.3
It has been known that the hemolymph node is one of the lymphoid organs found normally in ruminants and some rodents, and this organ shares morphological and functional characteristics of lymph node and spleen. To clanify the rigorous definition of morphological structures and functions of the hemolymph node in Wistar Kyoto(WKY)rats, we examined these organs of WKY rats gross anatomically and light microscopically. The hemolymph nodes were normally found in the abdominal cavity and in the neck of WKY rats. This organ was surrounded by a thin connective tissue capsule and there was a hilus. The parenchyma comprised a cortex of lymphatic nodules and diffuse lymphatic tissues, and a medulla of diffuse lymphatic tissues arranged in cords. Afferent and efferent lymph vessels were observed but there was no extensive subcapsular and medullary sinuses. These sinuses were filled with erythrocytes. The stroma of hemolymph nodes was composed of reticular cells and fibers, and many lymphocytes, granulocytes, erythrocytes, plasma cells, macrophages and megakaryocytes were supported by the reticular network. The above findings suggest that the hemolymph nodes of WKY rats may take part in blood formation, blood filtration and immune reaction.
박철범 ( Cheol Beom Park ) 한국동물실험대체법학회 2008 한국동물실험대체법학회 학술대회집 Vol.2008 No.1
Reconstructed skin provides advantages to single cell culture testing and leads to promising results as shown by different validation studies. In this study we investigated the efficacy of "KeraskinTM" compared with "EPI-200" and in vivo test. We conducted endpoint analysis including cell viability (MTT reduction, CCK-8) and IL-1 alpha release. Twenty chemical were investigated using keraskinTM model and compared with Epiderm model. Results of MTT assay and CCK-8 assay were not different between EPI-200 and KeraSkinTM, but IL-lα levels of some test items different between EPI-200 and KeraSkinTM. We concluded that this KeraSkinTM model is useful for alternative skin irritation test, although other tests should be conducted for many other chemicals in international validation study.
CB6F1-rasH2 transgenic mice를 이용한 발암성 시험 대체법
박은진 ( Eun Jin Park ),박선희 ( Sun Hee Park ),박찬성 ( Chan Sung Park ),김영선 ( Young Seon Kim ),김은애 ( Eun Ae Kim ),강종구 ( Jong Koo Kang ),서동석 ( Dong Suk Seo ),박철범 ( Cheol Beom Park ) 한국동물실험대체법학회 2008 동물실험대체법학회지 Vol.2 No.1
Alternative carcinogenecity test on CB6F1-rasH2 transgenic mice(rasH2) were conducted. Both sexes of rasH2 and wild type mice were orally administered at the dose of 1.44, 5.75 and 23 mg / kg of sibutramine mesilate and 20 mg / kg of sibutramine hydrochloride for 26 week. Incidence rate of tumor per mice and number of tumor were very low and not significantly different between control and treatment group. It means that sibutramine mesilate and sibutramine hydrochloride has no carcinogenecity on rasH2 mice and this result predicted that 2-year carcinogenecity of sibutramine mesilate would be negative. Based on these results, rasH2 transgenic mice has useful model for alternative of 2-year carcinogenecity test.
아플라톡신 4주간 반복투여에 의한 랫드 간발암 생체지표인 CYP450 1A1, p53 및 ras의 발현양상 변화
김대중(Dae Joong Kim),이범준(Beom Jun Lee),김태명(Tae Myoung Kim),이숙진(Sook Jin Lee),남상윤(Sang-Yoon Nam),홍진태(Jin Tae Hong),박철범(Cheol Beom Park),김철규(Cheol Kyu Kim),강종구(Jong-Koo Kang),윤영원(Young Won Yun) 한국실험동물학회 2004 Laboratory Animal Research Vol.20 No.4
We examined the relative toxicological potency of aflatoxins using biomarkers. Aflatoxins (AFB₁, AFB₂, AFG₁) at doses 10, 50, and 250 ㎍/㎏ were administerd twice i.g. per week for 4 weeks. Blood and serum biochemical analyses and histological and immunohistochemical examinations of p53, CYP450 1A1, glutathione S-transferase placental form (GST-P) were performed and expression of ras oncogene and level of the 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-OxodG) in the liver were measured. At 14 and 21 post-administration days, changes in the body weight of some aflatoxins-treated rats were found but they were not a dose-related response. There were no significant changes in organ weights and hematological values among all experimental groups. AFB₁ treatment at the dose of 250 ㎍/㎏ significantly decreased the levels of urea nitrogen and total bilirubin in serum but increased the levels of albumin, creatinine, and alanine transaminase (p<0.05). AFB₂ had no effects on blood biochemistry values but AFG₁ decreased the levels of aspartate transaminase in a dose dependent manner. The pathological lesions induced by aflatoxins in rat livers included massive vacuolar degeneration or lipidosis, centrilobular focal hepatic necrosis with inflammatory mononuclear cell infiltrations, and oval cell proliferation in the periportal areas. The severity of the damaged lesions was the most in the highest dose group of each aflatoxin B₁ treatment. The p53-positive hepatocytes were often observed at the high doses of aflatoxins. The Immuno-reactivity against CYP450 1A1 was mainly found in the centrilobular region of the liver at the high dose of AFB₁ and AFG₁. No GST-P foci in rat liver were observed in all experimental groups. The formation of 8-OxodG was not changed in the liver of rats treated with aflatoxins. The expression of ras oncogene in the rat liver by the AFB₁, AFB₂ and AFG₁ at some dosages was increased three to four folds compared to that of controls. These results suggest that CYP 450 1A1, p53, ras, GST-P and 8-OxodG as biomarkers were not useful to predict hepatocarcinogenicity of aflatoxins and develop a value of relative toxicity of aflatoxins vs AFB₁ in the present model. Further studies are needed to look for other biomarkers to predict carcinogenic potency of aflatoxins.