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박제권(Jae Kweon Park) 한국식품과학회 2020 식품과학과 산업 Vol.53 No.1
Most reports demonstrated the substrate specificitybased kinetic properties of chitin or chitosan degrading enzymes. However, there is virtually less information on the high quality and quantity production of chitin or chitosan hydrolysates having a larger than (GlcN)<SUB>7</SUB> from the hydrolysis of high molecular weight chitosan using specific enzymes and their biological activity. Therefore, the production of such molecules and the discovery of such enzyme sources are very important. Fortunately, the author has established a mass production method of chitosan hydrolysates (GlcN)<SUB>n</SUB>, n=2-13 that have been characterized as a potent antioxidant substance, as well as antifungal and antibacterial activities against Penicillium species and highly selective pathogenic bacteria. In addition, preclinical studies using (GlcN)<SUB>n</SUB>, n=5-25 demonstrated that these molecules played a very important role in maintaining biometric balance. Collectively, it is implicated that the application of these mixed substances to foods with significant biological activity is very encouraging.
탈지 미세조류 Botryococcus braunii 로부터의 수용성 다당의 항산화 활성과 항균활성
이다경,박제권,Lee, Da Gyung,Park, Jae Kweon 한국해양바이오학회 2015 한국해양바이오학회지 Vol.7 No.2
Sulfated polysaccharides from plants or seaweed are well known for antioxidant, anticoagulant activity or other biological activities. The aim of the present study is to optimize the condition for the isolation of water soluble polysaccharides (WSP) and evaluate the biological activity. WSP was isolated from defatted microalgae B. braunii using chlorosulfonic acid or sulfuric acid. Among WSP isolated using sulfuric acid showed the lowest the content of total carbohydrates (g/L), whereas showed the highest antioxidant activity. Also the preliminary structural analysis of WSP was performed by FT-IR spectroscopy analysis.
Bacillus safensis MA-01 유래 알파-만노사이데이즈의 효소학적 특성
이보미,김주원,박제권,Lee, Bo Mi,Kim, Joo Won,Park, Jae Kweon 한국해양바이오학회 2015 한국해양바이오학회지 Vol.7 No.1
An extracellular alkaline ${\alpha}$-D-mannosidase produced by a strain named as MA-01 was produced and its preliminary enzyme activity was characterized. Upon determining the 16S rDNA sequence and its homology search, the strain was identified to be one of species of the Bacillus safensis. Localization of enzyme was elucidated that ${\alpha}$-D-mannosidase can be found in culture medium as an extracellular enzyme. In addition, partial enzyme activity of 63% compared with the extracellular enzyme activity was observed in membrane protein. The optimal pH and temperature of the ${\alpha}$-D-mannosidase were pH 7.5 and $37^{\circ}C$, respectively. The $K_m$ and $V_{max}$ values of the ${\alpha}$-D-mannosidase in crude enzyme toward p-nitrophenyl-${\alpha}$-D-mannopyranoside were determined to be $455.6{\mu}M$ and $10.8{\mu}mole/min/mg$ of protein, respectively. To the best of our knowledge, this is the first report described the alkaline ${\alpha}$-D-mannosidase from the family of B. safensis.
이춘근,황유진,박제권,Lee, Choon Geun,Hwang, You Jin,Park, Jae Kweon 한국해양바이오학회 2014 한국해양바이오학회지 Vol.6 No.2
This study was investigated the antimicrobial activity of glutaraldehyde cross-linked glucosamine. Glutaraldehyde was used as a cross-linker which specifically combines an amine-group of molecules. To optimize the mixing ratio of glutaraldehyde and glucosamine, mixing ratio was set up 1:1, 2:1, 3:1 and 0.5:1 in molarity, respectively. The optimum mixing ratio of glucosamine and glutaraldehyde was found to be 3:1 using thin layer chromatography based on the production of complex. Glucosamine-glutaraldehyde cross-linked complex (Ggcc) revealed significant antimicrobial activity toward PWG than F1, both microbial strains were isolated from porcine semen as antibiotics resistance bacteria (ARB). These results clearly demonstrate that Ggcc has potential bactericidal activity toward ARB in porcine semen.
Penicillium italicum에 대한 중저분자 키토산의 항진균 효과
유아영 ( Ah Young Yoo ),박제권 ( Jae Kweon Park ) 한국키틴키토산학회 2014 한국키틴키토산학회지 Vol.19 No.3
키틴은 셀룰로스 다음으로 자연계에 풍부한 물질로서 친환경 소재로 식물에 비해 자원이 풍부하고 많은 효능이 입증되어 왔다. 본 연구에서는 이러한 키토산이 감귤에 푸른 곰팡이 병을 일으키는 P. italicum을 억제하는 친환경적 방제제로의 이용이 가능한지 여부를 확인하였다. 결과적으로 PD 액체 배지에서 다양한 분자량의 키토산은 모두 곰팡이 포자의 발아를 억제했다. 이후 키토산을 제거하고 새로 영양배지(PD)를 제공하였지만 키토산을 처리했던 포자는 3차 멸균수를 이용하여 키토산을 제거한 후에도 발아하지 않았다. 이러한 결과를 바탕으로 키토산은 포자 발아를 영구적으로 억제한다는 것을 확인하였다. 또한 PD 고체 배지에서 행한 disc 실험에 서는 5~7 kDa 범위의 중저분자 키토산이 가장 높은 효과를 보였다. 액체배양 조건에서는 CTSN과 CTSN-P는 각각 0.003%의 농도에서 포자를 저해했다. 결론적으로 여러 크기의 분자량을 갖는 중저분자 키토산은 강한 포자 발아 억제 능력을 나타냄으로서 감귤 저장이나 보관에 있어서 친환경적 방제제로서 매우 유용한 생물학적 소재가 될 수 있을 것으로 판단된다. The aim of this study was to evaluate the antifungal activity of chitosans with different molecular weight against Penicillium italicum which is known as a pathogenic fungus in citrus fruits. Antifungal activity of various molecular weight of chitosans was observed in morphological changes using microscope and was defined in disc diffusion assay. The result showed that various molecular weight of chitosan raging from 3 to 7 kDa completely inhibits the spore germination of P. italicum. These results indicate that medium size of chitosan can be used as a natural antifungal agent to control blue mold in postharvest citrus fruits.
Pseudomonas fluorescens JK-0412의 키틴 분해 효소 및 단백질 분해효소 생산 최적화
박용일 ( Yong Il Park ),서현효 ( Hyun Hyo Suh ),김우중 ( Woo Jung Kim ),고주희 ( Ju Hee Ko ),박제권 ( Jae Kweon Park ) 한국키틴키토산학회 2010 한국키틴키토산학회지 Vol.15 No.4
Pseudomonas fluorescens JK-0412가 생산하는 키틴 분해 효소와 단백질 분해효소의 생산성을 다양한 기질을 이용하여 실험조사 하였다. 각각 키틴 분해효소와 단백질 분해효소 (CTNase와 PRTase로서 명명)의 생산에 여러 가지 기질의 특이성을 검토하였다. 전자는 주로, pNP-GlcNAc에 기질 특이성을 보이는 CTNase, 그리고, 후자는 skim-milk에 기질특이 성을 갖는 PRTase로서 두 효소의 최적 생산 조건은 각각 pH8.0와 37℃였으며, 또한 1.0% dextran, 1.0% yeast extract, 1.0% MgSO4를 각각 carbon, nitrogen, and mineral 원으로서 이용했을 때, CTNase의 최대 생산성을 얻을 수 있었다. 또한 각각, 1.0% glucose, 1.0% ammonium sulfate, 1.0% MgSO4를 carbon, nitrogen, 및 mineral 원으로서 이용했을 때, PRTase의 최대 생산성을 얻을 수 있었다. 특히, 유일한 carbon과 nitrogen 원으로 chitin powder, chitosan, colloidal chitin 과 dextran을 기질로 이용하여 12일 동안 배양하여 효소활성을 측정한 결과, CTNase 생산에 있어 기질로 powder chitin를 사용하였을 경우 3일 이후 효소활성이 확인되었으며, PRTase 활성이 측정되기 시작한 4일 이후의 PRTase 생산에 있어서도 powder chitin이 가장 적합한 기질로 확인되었다. 따라서 상업적으로 적용할 수 있는 chitin은 수산가공 부산물로부터 얻을 수 있을 수 생물소재로서 CTNase와 PRTase 생산을 위한 생물 공학적인 공정에 이용가치가 매우 높다. The production of chitinolytic and proteolytic enzymes in submerged cultures of Pseudomonas fluorescens JK-0412 in media was investigated in experiment with pH, temperature and substrate concentration as the main parameters. The production of chitinolytic and proteolytic enzymes, named as CTNase and PRTase, the former has specific enzyme activity toward pNPGlcNAc mostly and the latter has specific enzyme activity toward skim-milk, was monitored under the influence at various concentrations of these substrates. The optimum conditions of pH, temperature and substrate concentration for the production of both enzymes was determined to be pH 8.0 and 37 o C, and pH 8.0 and 45 o C, respectively. The maximum production of CTNase was obtained when 1.0% dextran, 1.0% yeast extract and 1.0% MgSO4 were used as carbon, nitrogen and mineral sources in the absence of chitinous material. In addition, the maximum production of PRTase was obtained when 1.0% glucose, 1.0% ammonium sulfate, and 1.0% MgSO4 were used as carbon, nitrogen and mineral source, respectively. Furthermore, in the presence of flake chitin, chitosan, and colloidal chitin used as a sole carbon and nitrogen source, it was found that flake chitin was the most efficient elicitor of CTNase and PRTase after 3 and 4 day cultivation, respectively. Hence chitin powder is a suitable substrate for the production of both CTNase and PRTase to employ in an advanced biotechnological process, whereby several commercially applicable chitinous materials can be obtained from a waste product of the fishing industry.