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잣나무의 기내배양에 관한 연구 (Ⅰ) - Callus 의 유발과 생장 -
박용구,김우룡 ( Young Goo Park,Oue Ryong Kim ) 한국산림과학회 1983 한국산림과학회지 Vol.59 No.1
Embryo tissue from Pinus koraiensis was established in culture on G.D. and W.S. basal medium supplemented with the auxin (NAA, 2,4-D, IBA) and kinetin. The various combinations of growth regulators were studied in order to determine the specific requirements of the callus tissue in vitro. The inorganic nutrient combination of G.D. medium was found to be better than that of W,S. medium. G,D. basal medium supplemented with 0.1 ppm N.AA and 0.1 ppm kinetin was the most successful nutrient combination for the growth of callus induced from the embryo of P. koraiensis.
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잣나무의 기내배양에 관한 연구 . Ⅱ. Callus 세포의 염색체변이
김우룡,박용구 ( Oue Ryong Kim,Young Goo Park ) 한국산림과학회 1986 한국산림과학회지 Vol.74 No.1
Excised mature embryos of P. koraiensis were plated on a series of G.D. basic media supplemented with growth regulators as follow; 0.1 ㎎/ℓ NAA(M-1); 0.1 ㎎/ℓ NAA and 2,4-D (M-2); 0.1 ㎎/ℓ 2,4-D and BAP (M-3); 0.1 ㎎/ℓ 2,4-D and kinetin (M-4). Cytological study of the callus showed that thercentage of occurrance of diploid cells was observed 52% in M-3 and 36% in M-4, while that was revealed 29% in M-1 and 17% in M-1. The frequency of diploid cells was increased on the M-3 and M-4 media. The stable chromosome state is a crucial factor for organogenesis. Therefore, it can be inferred that the callus tissues cultured on media supplemented with both auxin and cytokinin have the greatest possibility of organogenesis.
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In Vitro Studies on Pinus koraiensis. II. Chromosomal Variation in the Callus
김우룡,박용구,Kim, Oue Ryong,Park, Young Goo Korean Society of Forest Science 1986 한국산림과학회지 Vol.74 No.1
GD 기본(基本) 배지(培地)에 서로 다른 생장조절물질(生長調節物質)을 첨가(添加)한 4 가지 배지(培地)에 잣나무 배(胚)에서 유래(由來)한 Callus를 1년(年) 6개월간(個月間) 배양(培養)시켜 염색체수(梁色體數) 변이(雙異)를 조사(調査)하였다. 그 결과(結果) $0.1mg/{\ell}$와 NAA $0.1mg/{\ell}$에 2, 4-D $0.1mg/{\ell}$를 첨가(添加)한 배지(培地) 즉(卽) Auun 단독(單獨) 및 배합(培合) 처리(處理)한 배지(培地)에서는 2n 비율(比率)이 각각(各各) 29%와 17%로 낮게 나타났다. 그러나 Auxin 과 Cytokinin 을 종합(複合) 처리(處理)한 배지(培地) 즉(卽) 2, 4-D $0.1mg/{\ell}$와 BAP $0.1mg/{\ell}$에서는 52 %, 2, 4-D $0.1mg/{\ell}$와 Kinetin $0.1mg/{\ell}$에서는 36 %로써 비교적(比較的) 높은 빈도(頻度)로 2n 세포(細胞)가 나타났다. 2n 세포(細胞)의 빈도(頻度)는 기관분화(器官分化)와 상관(相關)이 높은 것으로 생각 할 수 있었다. Excised mature embryos of P. koraiensis were plated on a series of G.D. basic media supplemented with growth regulators as follow; $0.1mg/{\ell}$ NAA(M-1); $0.1mg/{\ell}$ NAA and 2,4-D (M-2); $0.1mg/{\ell}$ 2,4-D and BAP (M-3); $0.1mg/{\ell}$ 2,4-D and kinetin (M-4). Cytological study of the callus showed that thercentage of occurrance of diploid cells was observed 52% in M-3 and 36% in M-4, while that was revealed 29% in M-1 and 17% in M-1. The frequency of diploid cells was increased on the M-3 and M-4 media. The stable chromosome state is a crucial factor for organogenesis. Therefore, it can be inferred that the callus tissues cultured on media supplemented with both auxin and cytokinin have the greatest possibility of organogenesis.
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