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- 저자 : 박옥이 ( Ok Yi Park ) (검색결과 5 건)
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조직도플러로 측정한 승모판륜속도를 이용한 좌심실 이완기능의 평가
박옥이 ( Ok Yi Park ),김용진 ( Yong Jin Kim ),배형준 ( Hyung Joon Bae ) 대한임상검사과학회 2002 대한임상검사과학회지(KJCLS) Vol.34 No.2
Mitral inflow velocity recorded by Doppler echocardiography has been widely used to evaluate left verrπicular diastolic function but it is affected by other factors. The mitral annulus velocity profile during diastole may provide additional information about left ventricular diasto1ic function. π1Is study was performed to assess the c1inical utility of mitral annulus velocity measured by Doppler tissue imaging (DTI) in the evaluation of left verrπicular diasto1ic function. Mitral annulus velocity during diastole was measured by DTI in 38 consecutive patients undergoing cardiac catheterization in whom mitral inflow velocity and time constant of isovolumic relaxation (tau) as well as mitral annulus velocity were measured simultaneously. Peak early diastolic mitral annulus velocity (r = -0.56, p < 0.01) and the earlyjlate ratio (r = -0.46, P < 0.01) correlated well with tau. When the combination of normal mitral inflow variables with pr이l onged tau (> or = 50 ms) was classified as pseudonormalization, peak early diastolic mitral annulus velocity < 8.5 cmfs and the earlyjlate ratio < 1 could identify the pseudonormalization with a sensitivity of 88% and specificity of 67%. Mitral annulus velocity determined by DTI is a relatively preload-independent variable in evaluating diastolic function.
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한국산 무당개구리(Bombina orientalis)의 Mitochondrial Cytochrome b Gene 변이 분석
이혜영,양서영,박옥이,이현익 한국유전학회 1999 Genes & Genomics Vol.21 No.3
Nucleotide sequences (390-base pairs) from the cytochrome b gene of mitochondrial DNA were analyzed to investigate genetic variation in Korean Bombina orientalis. Among all 10 individuals from 4 populations, 23 (5.8%) variable nucleotide sites were observed. The rate of interpopulational variation of nucleotide sequence was from 0.5 to 4.6%, while intrapopulational variation was from 0.0 to 1.0%. Based on Kimura's two parameter distance, the sequence divergences between populations were ranged from 0 to 0.0483. This result suggests that all Korean Bombina orientalis has relatively low genetic variations, which is corresponding with mtDNA RFLP and isozyme data.
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한국산 꼬리치레도롱뇽 Onychodactylus fischeri 의 핵형 분석
오세조,이혜영,양서영,진정화,박옥이 한국유전학회 1997 Genes & Genomics Vol.19 No.2
Karyotype analysis of Korean Onychodactylus fischeri was performed by using conventional Giemsa and nucleolus organizer regions (NORs) staining methods. The diploid karyotype consists of 2n=72 chromosomes, including 6 pairs of large chromosomes, 7 pairs of medium size ones, 25 pairs of microchromosome and arm number is 102. A pair of NORs was located adjacent to the centromeric region of microchromosomes.
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한국산 무당개구리(Bombina orientalis)Mitochondrial DNA 의 절편 양상 및 변이
오세조,이혜영,양서영,진정화,박옥이 한국유전학회 1996 Genes & Genomics Vol.18 No.2
The mtDNA of Korean Bombina orientalis collected from 12 natural populations were digested with 12 restriction enzymes and were analyzed for nucleotide sequence variations. The average genome size of mtDNA was 18.0±0.1 kb. Digested mtDNA fragment variations within population were observed by enzymes BcA, Clal, Pstl, Pvull, SaA, Xhol. Fragment variations between populations were also seen in the fragments digested by BcA, BstEll, Clal Pstl, Pvull, SaA or Xhol. Especially, a higher than usual fragment variations were found in the fragments digested by BcA and Pstl restriction enzymes. These variations within and between populations are attributed to the presence or absence of recognition sites by base substitution. The comparisons of restriction fragments enabled the estimation of the levels of fragment homology (F value) and nucleotide sequence divergence (p value). The mean F and p value of interspecific variation were 0.674±0.14 and 0.023±0.013. The p value of vertebrates was about 5% in general but that of Korean Bombina orientalis was shown about 2% in this study. The result suggests that Korean Bombina showed lower interspecific variation than that of other vertebrates.
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한국산 청개구리(Hyla japonica)Mitochondrial DNA 의 집단간 및 집단내 유전적 변이
오세조,이혜영,양서영,진정화,박옥이 한국유전학회 1996 Genes & Genomics Vol.18 No.1
Mitochondrial DNAs (mtDNAs) of Korean Hyla japonica(11 populations) and Japanese H. japonica(1 population) were analysed to investigate inter- and intrapopulation of genetic variation. Purified mtDNA of Korean H. japonica were digested with each of 11 restriction enzymes and 8 enzymes for Japanese H. japonica mtDNA. The average genome size of mtDNA was 20.30±0.3kb in Korean species, while 20.07±0.8kb in Japanese one. For the analysis, level of fragment homology (F) and nucleotide sequence divergence (p) were estimated from comparisons of digestion profiles. Among 11 populations of Korean species. the average F and p values were 0.722±0.15 (0.246-0.970) and 0.020±0.01 (0.002-0.084) while between Korean populations and Japanese one they revealed 0.172±0.04 (0.114-0.225) and 0.109±0.01 (0.082-0.133), respectively. The nucleotide sequence divergence between 11 populations of Korean species was 2% which indicated low genic variation among populations. However, the value between Korean and Japanese populations revealed more hig-her. 11.5% which indicated that comparison of H. japonica from two countries showed higher genic variations.
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