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박동헌,장현용,박춘근,정희태,김정익,양부근 한국발생생물학회 2004 발생과 생식 Vol.8 No.2
본 연구는 임신한 생쥐에 DEHP의 투여가 자손의 번식특성과 혈액성분에 미치는 영향을 검토하였으며 그결과는 다음과 같다. 임신기에 DEHP의 투여가 웅성 자손의 체중, 번식기관 무게 및 정액 성상에 미치는 결과는 투여구간에 커다란 차이가 없었다. 임신기에 DEHP의 투여가 웅성 자손의 혈액 성상에 미치는 영향을 조사한 결과, WBC, HB, HT, MCH 및 albumin은 투여구간에 차이가 없었으나, RBC MCV, MCHC, PLT 및 total p The objective of this study was to assess that the effects of DEHP administration on reproductive characteristics and blood hematological and chemical values in pups born after DEHP administration in pregnant mice. DEHP was administrated to pregnant mice by intraperitoneally injection with 0, 0.5, 1.0 and 10.0mg/kg B.W, 5 times at 3 days interval from Day 1 to Day 16 in the gestation period. The body weight and reproductive organ weight(testis, epididymis and coagulating gland) in male pups on 45 day after birth was not affected in all experimental groups, but vesicular gland in DEHP groups was significantly lower than that of control group(P<0.05). The semen characteristics of male pups were not affected in DEHP treatment groups. The WBC, HB, HT, MCH and albumin values in male pups were not affected in all experimental groups, but RBC MCV, MCHC, PLT and total protein values were significantly different among the experimental groups(P<0.05). In female pups, the effects of DEHP administration were not affected the body and uterus weight, but the left ovary in 10.0mg DEHP group was significantly heavier than in control and 0.5mg DEHP group(P<0.05). The WBC, MCV, MCH, MCHC, PLT, albumin, BUN and total protein values in female were not different in all experimental groups. The RBC, HB and HT values were significantly different among the experimental gruop(P<0.05). The historical evaluation of testis in male pups that were grown to 45 days after birth was not different in all experimental groups. The ovary in female pups had many corpus luteum in 10.0mg DEHP group. The endometriosisi of uterus was significantly decreased in DEHP group. There results suggest that low concentration of DEHP administration in pup born after DEHP administration in pregnant mice was not affered on reproductive characteristic, but was affected on blood hematological and chemical values.
BPA와 DEHP가 돼지 정액 성상에 미치는 독성 영향
박동헌,장현용,정희태,김종택,박춘근,김정익,양부근 한국동물생명공학회(구 한국동물번식학회) 2006 Reproductive & developmental biology Vol.30 No.3
본 연구는 내분비계 장애물질인 BPA와 DEHP가 돼지 정액 성상(운동성, 생존율, 원형질막의 정상성, 기형율)에 유해한 영향을 미치는지를 검토하였다. 일정 농도로 희석한 돼지 정액에 BPA와 DEHP를 각각 0, 1, 10, 100 μM의 농도로 처리하여 3, 6, 9시간 동안 체외배양을 실시하였다. BPA 처리에 따른 운동성과 생존율은 체외배양 시간과 첨가농도에 따라 감소하였으며, 체외배양시간이 경과함에 따라 대조구와 명백한 차이를 나타냈다. 100 μM의 농도로 처리한 경우의 운동성과 생존율은 체외배양시간에 관계없이 처리군이 대조구에 비해 유의적으로 감소하였다(p<0.05). DEHP 처리에 따른 정자의 운동성과 생존율도 체외배양시간과 첨가농도에 따라 감소하여 BPA의 성적과 유사한 경향을 나타냈다. 정자원형질막의 기능성은 배양시간에 따라 감소하였으며 BPA 및 DEHP 첨가농도 간에 차이가 인정되었으며, 특히 100 μM 처리구에서 급격히 감소하는 성적을 나타났다. 정자의 기형율은 BPA 및 DEHP 처리시간 및 농도에 크게 영향을 받지는 않았다. 본 연구의 결과로 볼 때, 고농도의 BPA와 DEHP(>10 μM)에 정자의 장시간 노출은 유해한 영향을 줄 것으로 사료된다. The objective of this study was to investigate the toxic effects of Bisphenol A(BPA) and di-2 ethyhexyl phthalate (DEHP) as endocrine disrupters on sperm motility, viability, membrane integrity and abnormality during in vitro incubation of boar semen. Semen were randomly divide into 24 groups and treated with different concentrations of BPA and DEHP (1~100 μM) for 3, 6 and 9 hrs, respectively. The percentages of sperm motility and viability decreased by treatment time with both BPA and DEHP, and obiously differ from the controls. The percentages of sperm motility and viability significantly decreased by incubation with both 100 μM of BPA and DEHP compared to control and other treatment groups (p<0.05). Sperm membrane integrity was significantly reduced by incubation with 10 and 100 μM of BPA and DEHP, respectively (p<0.05), but sperm abnormality were not significantly affect both BPA and DEHP. These results indicate that high concentration of BPA and DEHP (>10 μM) can affect noxiously the sperm characteristics.
박동헌,장현용,박춘근,정희태,김정익,양부근 한국발생생물학회 2004 발생과 생식 Vol.8 No.2
본 연구는 임신한 생쥐에 DEHP의 투여가 분만 후 생쥐의 번식기능과 태아의 성비에 미치는 영향을 검토하였다. 임신한 자성 생쥐에 DEHP을 투여한 후 임신 19일째에 임신한 자성의 체중, 태아의 체중, 태아수 및 태아성비를 조사한 결과, 실험개시시와 실험종료시 임신한 자성의 체중은 각 투여구간에 차이가 없었으며, 자웅성태아의 체중, 평균 태아수 및 태아의 자웅성 성비는 투여구간에 커다란 차이가 없었다. 임신기에 DEHP의 투여가 분만한 후 어미의 번식 This study is illustrated that 1) the effects of DEHP administration on body weight, sex ratio and litter size on 19 days in the prenatal period 2) the effects of reproductive organ weight and blood hematological values on 25 days postpartum in pregnant mice. DEHP was administrated to pregnant mice by intraperitoneal injection at 0, 0.5, 1.0 and 10.0mg/kg B.W from day 1 to day 16 in the gestation period with 5 times at 3 days interval. There were no significantly differences in the material body weight, litter size and sex ratio on 19 days in the prenatal period. The body weight of the fatal male mice was slightly lower in 1.0 and 10.0mg DEHP groups than in any other groups. The reproductive organ weight and hematological values in dam mice on 25 days postpartum were not affected by DEHP administration. The histological evaluation of ovary in dam mice on 25 days after dilivery was not different in all experimental groups, but the endometriosis and edema of uterus in dam mice were significantly decreased in 0.5mg DEHP group than that of control group.These results indicate that the administration of low concentration of DEHP was not affected on reproductive characteristic and blood hematological values in pregnant mice.
박동헌,양부근,김종복,전기준,정희태,박춘근,이성수,박노형,원유석 한국동물번식학회 1999 Reproductive & developmental biology Vol.23 No.3
The present study was designed to examine the effects of selenium(Se), vitamin E(Vit. E) and recombinant Bovine Somatotropin(rBST) administration on the selenium and vitamin E concentrations of blood in Hanwoo sires Hanwoo sires were randomly assigned to five groups; 1. control, 2. rBST, 0.09mg/kg body weight (BW) 3. Vit E, 1,500IU/kg BW, 4. Se 0.lmg/kg BW, 5. Vit E, 1.500IU plus Se 0.1mg/kg BW. rBST, Vit. E and Se for each experimental group were given 6 times at 15 days interval by intramuscular injection. Blood samples were collected 10 times for experimental periods, separated the serum by centrifugation, and stored at -7$0^{\circ}C$. Se and Vito E concentrations in blood were measured by fluorophotometer and HPLC. Se concentrations of blood in control, rBST, Vito E, Se and Se plus Vito E groups were 64.55, 65.50, 68.15, 73.11 and 74.09 ppb/$m\ell$, respectively. Se concentration in Vit. E plus Se group was significantly higher than in control and rBST groups (P<0.05), but Vito E group was not significantly different in control and rBST groups(P>0.05). The Vit. E concentrations of blood in control, rBST, Vit. E, Se and Se plus Vit. E groups were 2.27, 2.32, 2.80, 2.58 and 2.75 ppm/$m\ell$, respectively. Vit. E and Vit. E plus Se groups were slightly higher than those of any other groups, but not significantly difference in 외 I experimental groups(P<0.05). These results indicate that Se and Vit. E concentrations of blood were slightly increased with the injection of Se and Vit. E in Hanwoo sires.