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식물성 오일로부터 Ralstonia eutropha의 polyhydroxyalkanoates 합성 특성
박대후(Dae Hoo Park),김범수(Beom Soo Kim) 한국생물공학회 2010 KSBB Journal Vol.25 No.3
식물성 오일 또는 glycerol로부터 Ralstonia eutropha 여섯 균주의 polyhydroxyalkanoates (PHA) 합성 특성을 조사 하였다. 탄소원으로 soybean oil, olive oil, 또는 glycerol 만을 공급시 poly(3-hydroxybutyrate) homopolymer가 생성되었으며, γ-butyrolactone 또는 pentanoic acid를 함께 공급시 poly(3-hydroxybutyrate-co-4-hydroxybutyrate) 또는 poly(3- hydroxybutyrate-co-3-hydroxyvalerate) copolymer가 각각 합성되었다. 최종 균체농도 및 PHA 함량면에서 최적인 균주는 R. eutropha KCTC 2662로 결정되었으며, 최적 기질인 soybean oil 20 g/L로부터 72 h에 균체농도 1.7~9.2 g/L, PHA 함량 70~92 wt%를 얻을 수 있었다. Six strains of Ralstonia eutropha were grown to investigate characteristics of polyhydroxyalkanoates (PHA) synthesis from vegetable oils or glycerol. Poly(3-hydroxybutyrate) homopolymer was formed using soybean oil, olive oil, or glycerol as carbon source, while poly(3-hydroxybutyrate-co-4-hydroxybutyrate) or poly(3-hydroxybutyrateco- 3-hydroxyvalerate) copolymers were synthesized by co-feeding γ-butyrolactone or pentanoic acid, respectively. Optimum strain was determined as R. eutropha KCTC 2662 in terms of final cell concentration and PHA content. From 20 g/L of soybean oil (optimum substrate), cell concentration and PHA content at 72 h ranged 1.7~9.2 g/L and 70~92 wt%, respectively.
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박대후 ( Dae Hoo Park ),김범수 ( Beom Soo Kim ) 충북대학교 산업과학기술연구소 2009 산업과학기술연구 논문집 Vol.23 No.2
Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] can be tailored to achieve a variety of polymeric materials, from hard crystalline plastics to very elastic rubber by varying 4HB monomer composition. In this study, Ralstonia eutropha ATCC 17699 was grown to produce P(3HB-co-4HB) copolymer using a mixture of soybean oil and γ-butyrolactone. The effect of soybean oil and γ-butyrolactone on cell growth was investigated by varying the concentrations in flask cultures. Cell growth was inhibited by high concentrations of γ-butyrolactone over 10 g/L. 4HB fraction was controlled at 0 to 50 mol% by changing the ratio of soybean oil to γ-butyrolactone. Increasing the concentration of γ-butyrolactone in the culture medium resulted in an increase of the 4HB fraction in the copolyester. In 2.5 L fermentor batch culture, cell concentration and 4HB fraction obtained were 5.5 g/L and 13.8 mol%, respectively.
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