http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
RANKL에 의해 유도되는 뼈파괴세포 분화에 맥문동의 효과
박기인(Kie-In Park),오재민(Jaemin Oh) 대한체질인류학회 2011 해부·생물인류학 (Anat Biol Anthropol) Vol.24 No.4
뼈파괴세포의 생성이나 활성 증가는 원하지 않는 뼈 흡수를 야기한다. 뼈파괴세포의 분화와 기능을 억제하는 천연물에 대한 연구가 뼈엉성증성 뼈질환의 치료와 예방을 위해 진행중이다. 맥문동은 동양의학에서 진해, 거담작용, 구갈 작용을 억제하는 작용 및 강장작용, 혈당 조절 작용, 이뇨작용, 변비를 조절하는 데 사용되어 왔다. 또한 최근에는 항염증 작용과 항혈전작용, 항유착작용이 있는 것으로 보고되고 있다. 본 연구의 목적은 맥문동이 뼈파괴세포 분화에 미치는 영향과 그 기전을 알아보기 위한 것이다. 맥문동 물 추출물은 RANKL에 의해 유도되는 큰포식세포의 뼈파괴세포로의 분화를 농도 의존적으로 억제하였다. 그러나 맥문동 추출물은 세포 독성은 나타내지 않았다. c-Fos, NFATc1, tartrate resistant-acid phosphatase (TRAP), cathepsin K의 mRNA의 발현이 맥문동 추출물에 의해 억제되었다. 또한 RANKL에 의해 유도되는 c-Fos, NFATc1의 단백질 발현도 저해되었다. 신호 경로 중에서 맥문동 추출물은 RANKL에 의해 유도되는 p38의 인산화를 저해하였다. 그러므로, 맥문동은 뼈파괴세포 분화에 필수 유전자인 c-Fos, NFATc1의 발현을 억제함으로 뼈파괴세포 분화를 억제한다. 결론적으로 이러한 결과는 맥문동이 특이한 독성 없이 뼈엉성증을 치료하는 유용한 후보물질이 될 수 있음을 암시하는 것이다. Increased formation and activation of osteoclast lead to unwanted bone resorption. Several natural products which have inhibitory effects on osteoclast differentiation and function are under investigation to prevent and treat the osteoporotic bone disease. Liriopis tuber has been used in Oriental medicine for the suppression of cough, expectoration, thirst, and has been used for sthenia, diuresis, blood glucose regulation, treatment of xerostomia, and constipation. Also, recently it has been reported that Liriopsis tuber has anti-inflammatory, anti-thrombic, anti-adhesive activities. The purpose of this study was to evaluate the effects of Liriopis tuber on osteoclast differentiation and was to evaluate of its mechanism. Water extract of Liriopis tuber significantly inhibited receptor activator of nuclear factor-kB ligand (RANKL)-induced osteoclast differentiation in bone marrow macrophages (BMMs) in a dose dependent manner. However, water extract of Liriopis tuber did not affect cytotoxicity when compared with control. The mRNA expression of c-Fos, NFATc1, tartrate resistant-acid phosphatase (TRAP), and cathepsin K induced by RANKL was inhibited by water extract of Liriopis tuber treatment. Also, water extract of Liriopis tuber inhibited the protein expression of c-Fos and NFATc1 expression in BMMs treated with RANKL. Among the signal pathways, water extract of Liriopis tuber suppressed the phosphorylation of p38 induced by RANKL. In summary, Liriopis tuber exerted inhibitory effects on osteoclast diffentiation via suppression of c-Fos and NFATc1 which are essential gene to osteoclastogenesis. Taken together, these results suggest that Liriopis tuber may be a useful candidate in the treatment of osteoporosis without special toxicity.
Vitreoscilla Catalase 의 분리 및 특성에 관한 연구
박기인,박충웅 ( Kie In Park,Chung Ung Park ) 생화학분자생물학회 1994 BMB Reports Vol.27 No.6
Vitreoscilla is a gram-negative bacterium that contains a unique bacterial hemoglobin and grows very well under the condition of low oxygen concentration. It also contain a bacterial catalase to be not correspond with another species on genus Beggiatoa. The primary function of Vitreoscilla catalase may be to remove hydrogen peroxide produced by ViWb oxidation. The molecular size of the catalase was estimated to be approximately 250,000 Da. The subunit structure of this enzyme may be A₂B₂ (A : MW 64,000 Da, B : MW 58,000 Da) but is not clear in the research reported here. Optimum pH is 7.0∼8.0 for catalase activity and Soret peak on absorption spectra of oxidized catalase is represented in 406 ㎚ and Soret peak of reduced form from sodium dithionite moved at 442 ㎚. Vitreoscilla catalase is unstable a high tempernture, and its Michealis constant, K_m was 0.016 M hydrogen peroxide. The turnover number of the enzyme was 25,000 mol. The 0.25 mM potassium cyanide was competitive inhibitor and the dissociation constant of the enzyme-inhibitor complex was 0.67 mM. N-terminal amino acid sequences of two subunits are determined for the probe synthesis using to the cloning of Vitreoscilla catalase gene.
PC12 세포 신경전달물질 방출 저해제 S9940이 신경세포 돌기신장에 미치는 영향
이윤식,박기인,Lee, Yun-Sik,Park, Kie-In 한국독성학회 1998 Toxicological Research Vol.14 No.3
We identified S9940, a novel microbial metabolite from Streptomyces spp., to inhibit the release of neurotransmitter from PC12 cells. S9940 is an inhibitor of trifiated norepinephrine ([$^{3}H$]-NE) release in high $K^+$ buffer solution containing ionomycin, indicating that S9940 inhibits neurotransmitter release after the influx of $Ca^{2+}$ ions. We also examined the effect of S9940 on $\beta-glucuronidase$ release from guinea pig neurophils and the effect on the neurite extension of PC12 cells and rat hippocampal neurons. As a result, S9940 inhibited $\beta-glucuronidase$ release: when treated with $5{\mu}g/ml$ of S9940, which prevented [$^{3}H$]-NE release, the inhibition of neurite extension for both PC12 cells and rat hippocampal neurons was observed.
PC12 세포에서 신경전달물질 방출을 저해하는 물질 S9940 물질의 탐색
이윤식,박기인,Lee, Yun-Sik,Park, Kie-In 한국독성학회 1998 Toxicological Research Vol.14 No.3
We established an in vitro experimental system using the following procedure. We first introduced tritium-labelled norepinephrine ([3H]-NE)into PC12 cells. The [3H]-NE incorporated into PC12 cells were then stimulated by a high concentration (60 mM) of $K^+$ during 12 minutes. Then, we counted the amount of [3H]-NE release from PC12 cells with the scintillation counter. After screening fungal, Streptomyces or bacterial product using this experimental system, we obtained S9940 from Streptomyces spp. which inhibited [3H]-NE release from PC12 cells. S9940 also inhibits the release of ATP as a neurotransmitter of PC12 cells and rat cortical neurons. The inhibitory effect was seen even when the PC12 cells were treated with low $K^+$ buffer containing ionomycin $(1\muM)$ as an ionopore. This result suggests that the inhibitory action of S9940 on neurotransmitter release appeared after the influx of $Ca^{2+}$.