http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
랫드의 혈액응고 및 혈소판 응집에 미치는 은나노 입자의 영향
박광식(Kwangsik Park),이연진(Yeonjin Lee) 大韓藥學會 2012 약학회지 Vol.56 No.6
Effects of citrate-capped silver nanoparticles (AgNPs) on the blood coagulation and platelet aggregation were investigated using whole blood, platelet rich plasma (PRP) and washed platelet obtained from SD male rats. To confirm the stability of AgNPs in the test, size distribution of the nanoparticles was measured in the vehicles including distilled water, serum, and platelet buffers. The average size of AgNPs was 20 nm in the vehicles, which means that the stability was maintained during the whole experimental period. When blood coagulation was monitored by using whole blood impedance aggregometer, coagulation was not observed at the concentration of 1, 10 and 50 ppm. Platelets in plasma or in buffer were not aggregated by AgNPs at the concentration of 1, 2 and 4 ppm, respectively. The test concentration of AgNPs could not be increased because the dark color of the nanoparticles impeded the transmission of light, which is an indicator of aggregation. Although the blood or platelets were pre-activated by collagen, thrombin, or ADP with sub-threshold level, aggregation was not observed at the test concentration. Microscopic observation also supported the result obtained by the aggregometer.
박광식(Kwangsik Park) 환경독성보건학회 2008 환경독성보건학회지 Vol.23 No.4
Effects of titanium dioxide nanoparticles on blood coagulation and platelet aggregation were investigated using diluted whole blood and platelet rich plasma (PRP) solution prepared from human and rat blood, respectively. Blood coagulation was monitored by using a whole blood impedance aggregometer and titanium dioxide nanoparticle (10, 20, and 40 ppm) did not show any effect on the coagulation both in human and in rat blood. When platelet aggregation was measured by turbidometric method after addition of titanium dioxide nanoparticles to PRP solution with final concentrations of 1, 5 and 10 ppm. no aggregation was observed.
Juyoung Park(박주영),Handule Lee(이한들),Kwangsik Park(박광식) 환경독성보건학회 2021 한국독성학회 심포지움 및 학술발표회 Vol.2021 No.5
Some chemicals commonly used in personal care products, household items, food vessels, cosmetics, and other consumer products are potentially harmful, and several reviews of epidemiological studies have suggested the associations between the chemical exposure from consumer products, and respiratory diseases, skin sensitization, and reproductive problems. Therefore, risk assessment is essential for management of consumer products safety. Necessarily, the estimation of human exposure is an essential step in risk assessment, and the absorption rate of those chemicals via the gastrointestinal tract, respiratory tract, and skin are very critical in determining the internal dose of the exposed chemicals. In this study, parallel artificial membrane permeability assays (PAMPA) for the gastrointestinal tract and skin were performed to evaluate the permeability of parabens, bisphenols, isothiazolinones, and phthalates. Lipid solubility of test chemicals indicated by log P values was shown as the most critical factor and showed a positive association with the permeability of parabens, bisphenols, and isothiazolinones in PAMPA assay. However, phthalates showed a reverse-association between lipophilicity and permeability. The permeability of all the tested chemicals was higher in the gastrointestinal tract membrane than in the skin membrane. The pH in donor solution did not show significant effects on the permeability in all the chemicals, except the chemicals with a free hydrophilic moiety in their chemical structures.
티타늄나노입자의 랫드 5일 반복 경구투여 후 배설, 조직분포 및 독성에 관한 평가연구
김혜진,박광식,Kim, Hyejin,Park, Kwangsik 한국환경보건학회 2014 한국환경보건학회지 Vol.40 No.4
Objectives: Excretion and tissue distribution of titanium oxide nanoparticles were evaluated in rats after oral administration. The relation between toxicity and systemic concentration of nanoparaticles was investigated. Methods: Rats were orally treated with titanium oxide nanoparticles (10, 100 mg/kg) for five consecutive days. General toxicity, blood chemistry, and serum biochemical analysis were analyzed. Titanium concentration in liver, kidney, lung, urine and feces were measured and histopathology was performed in these organs. Results: Induction of toxicological parameters was not observed and titanium nanoparticles were excreted via feces. Conclusion: Absorption of titanium oxide nanoparticles via the gastrointestinal tract after oral administration was very poor and systemic concentration of titanium oxide nanoparticles was not elevated. Titanium oxide nanoparticles did not cause toxicities in rats after oral administration.