그늘쑥의 식물화학적 성분

문형인,권학철,지옥표,이강노,Moon, Hyung-In,Kwon, Hak-Cheol,Zee, Ok-Pyo,Lee, Kang-Ro 한국생약학회 1999 생약학회지 Vol.30 No.1

Two carotenoids and four flavonoids were isolated from the whole plant of Artemisia sylvatica Max. Their structures were identified as ${\beta}-carotene$, lutein, luteolin, rutin, $pectolinarigenin-7-O-{\beta}-glucopyranoside$ and luteolin-7-O-neohesperidoside on the basis of spectroscopic means.

백서에서 Depulpin®과 Formocresol에 대한 치수와 치근단 조직의 반응

문형인,김선호,황윤찬,오병주,황인남,김선헌,정선와,윤창,오원만 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.4

One fifth dilution of formocresol is usually for pulpotomy of the primary teeth and emergency pulpotomy of the permanent teeth. However, the use of formaldehyde has been subjected to criticism because it may be absorbed into the blood stream and become distributed systemically, it may also alter the pulp tissue rendering it immumologically active, and have carcinogenic potential. Recently Depulpin®(VoCo., Germany) gains popularity as a devitalizing agent during root canal therapy in spite of high concentration of 49% paraformaldehyde because it facilitate devitalization of pulp and make root canal therapy easier. But there have been not enough publications about the reaction of pulp and periapical tissue caused by Depulpin. This study was performed to evaluate the histological changes in pulp and periapical tissue of rats after pulpotomy using formocresol and Depulpin and to elucidate the toxic effects of these agents. Thirty six Sprague-Dawley rats were anesthetized by intraperitoneal injection of ketamine. Maxillary first molar teeth were used for pulpotomy with formocresol and Depulpin. Rats were sacrificed after 2 days, 4 days, 1 week, 2 weeks, 3 weeks and 4 weeks respectively. Specimens were histologically observed by light microscope changes in pulp and periapical tissue. The obtained results were as follows. 1. Formocresol group A zone of fixed tissue, in which odontoblasts could clearly be defined, was present directly underneath the pulpotomy dressing in almost all teeth of this group. This was followed by an area of necrotic tissue which resembled dried out fibrous tissue with no cellular detail except some pyknotic nuclei. In the specimens of after 2 days, 4 days, 1 week, 2 weeks in which vital tissue was present. it was separated from the fibrous area by a zone of inflammation. In the specimens of after 3 weeks and after 4 weeks, inflammatory infiltrate was in the periodontal ligament adjacent to the apical foramina of the teeth. 2. Depulpin® group The area of necrotic tissue which had no cells and fibers , was present adjacent to the dressing. This was followed by dried out fibrous tissue with no cellular details except some pyknotic nucleli. A short stump of vital pulp with odontoblasts was present at the end of the canal after 2 days. Inflammatory infiltrate was in the periodontal ligament after 4 days and after 1week. Severe root resorption and necrosis of periapical tissue opposite the root resorption site were defined after 2 weeks and after 3 weeks. Periapical lesion which consist of necrotic tissue surrounded by a fibrous connective wall. was found after 4 weeks. The results indicated that Depulpin can cause more adverse reaction to the dental pulp and periapical tissue than formocresol, and further studies are needed for its clinical use with safety.

Anticancer Compounds of Plantago asiatica L.

문형인,지옥표 한국약용작물학회 1999 한국약용작물학회지 Vol.7 No.2

차전자의 메탄올추출물을 헥산, 클로로포름, 에틸아세테이트, 부탄올로 계통분획하여, 활성을 검정하고, 그 중 활성분획인 헥산과 에틸아세테이트 분획을 크로마토그라피하여 4종의 화합물을 분리하였으며, 각종 기기분석과 이화학적 분석을 통하여 β-sitosterol(C1), cholest-5-en-3β-ol(C2), rutin(C3), coumarin-7-O-β-glucopyranoside(C4)임을 확인 하였으며, 4종의 화합물 중 cholest-5-en-3β-ol, rutin(C3)이 주요 항암 성분 이었다. The cytotoxicity-guided fractionation of the seed of Plantago asiatica extracts led to the isolation of four compounds, responsible for the cytotoxicity against four human tumor cell lines, i. e., A431 (Human Epidermoid carcinoma), KHOS-NP (Human Osteosarcoma). SNU-1 (Human stomach carcinoma), SNU-C4 (Human large intestine carcinoma). The structure were elucidated by the phsyco chemical data: β-sitosterol(C1), cholest-5-en-3β-ol(C2), rutin(C3), coumarin-7-O-β-glucopyranoside(C4). IC50 values of compound C2 were 14.6, 13.5, 10.3, 17.8 μg/ml, and compound C3 and C4 showed activity, having IC50 values ranging from 10.3 to 20.14 μg/ml.

옥미수의 분획이 알코올 및 알콜대사효소에 미치는 효과

문형인,HyungInMoon 한국자원식물학회 1997 한국자원식물학회지 Vol.10 No.4

Spraque-Dawley계 응성흰쥐에 계통분획한 cornsilk의 각 분획물을 경구투여하고 혈청 Ethanol농도와 간의 Alcohol dehydrogenase활서에 미치는 효과를 검색 추적한 결과 알코올대사를 촉진시키는 성분은 주로 에탄올가용부에, 억제시키는 성분은 에탄올불용부에 주로 존재함을 추정할 수 있었다. Effect of various fraction from corn silk on alcohol metaholism in rats were examined and the results were as follows: ethanol souhle fraction. after a single oral administration to rats. was found to cause a significant deL'I'ease in the serum ethanol concentration as well as enh.lJ1cement of liver eytosolie ADH activity, on the other hand. the fraction imouhle in ethanol was found to cause an increase in the blood ethanol concentration and inhihit ADH activity.

소엽(蘇葉)의 추출물이 혈장알콜농도와 간의 알콜대사효소에 미치는 영향

문형인,지옥표,신국현 韓國藥用作物學會 1998 한국약용작물학회지 Vol.6 No.2

Spraque-Dawley계 웅성흰쥐에 계통분획한 소엽의 각 분획물을 경구투여하고 혈청 Ethanol농도와 간의 ADH활성에 미치는 효과를 검색 추적한 결과 알코올대사를 촉진시키는 성분은 주로 에탄올가용부에, 억제시키는 성분은 에탄올불용부에 주로 존재함을 추정할 수 있었고 현재 활성성분을 분리중에 있다. Effects of organic solvent fractions from perilla ethanol extract on alcohol metabolism in rats were examined and the results were as follows: Ethanol souble fraction, after a single oral administration to rats, was found to cause a significant decrease in the serum ethanol concentration as well as enhancement of liver cytosolic alcohol dehydrogenase (ADH) activity. On the other hand, the fraction insouble in ethanol was found to increase ethanol concentration in the blood and inhibit ADH activity.

생강과 생강나무의 향기성분조성 비교

문형인,이재학 韓國作物學會 1997 한국작물학회지 Vol.42 No.1

생강나무의 향기성분 조성을 분석해 본 결과는 다음과 같다. 1. GC상에서 생강나무는 꽃, 잎, 줄기 각각 60, 80, 83개의 peak가 관찰되었으며, 주요 성분군은 생강나무의 경우는 꽃의 경우는 sabinene, eta -myrcene, 1-limonene, cir-3-hexanal, λ -terpinene, (Z)-3-hexen-1-ol acetate, λ -elemene, 1-borneol, δ -guaiene, ledene, δ -ca-dinene, elemol, 9-octadecanal, 1-(1,5diNe-4-hexenyl ) -4-mebenzene, α -chamigrene, λ -selinene, β-endesmol, 1-phellandrene, 3-Me-6-(1-Me, ethyl ) -2-cyclohexen-1-one, epiglobulol의 성분이, 잎의 경우는 phellandrene, α -terpinolene, sabinene, β-myrcene, ι-limonene, cis-3-hexanal, λ -terpinene, (Z)-3-hexen-1-ol acetate, λ -elemene, 1-borneol, δ -guaiene, ledene, δ -cadinene, olemol, 9-octadecanal, λ -selinene, o-chamigrene, 1-(1,5-diMe-4-hexenyl) -4-mebenzene, β-endesmol의 성분이, 줄기의 경우는 sabinene, β-myrcene, ι-limonene, phellamdrene, α -terpinene, ledene, 1-borneol, λ -terpinene, 2,4a,5,6,7,8,9,9a-octahydroben-zocycloheptane, elemol, λ -selinene, cis-3-hexanal, (Z)-3-hexen-1-ol acetate, λ -elemene, δ -guaiene, δ -cadinene, 9-octadecanal, 1-(1,5-diMe-4-hexenyl) -4-mebenzeno, β-endesmol, α -charugrene의 향기성분이 주요 성분군으로 확인되었다. 2. 생강나무에서 생강의 향기를 발산하는 성분으로는 β-myrcene, o-terpinolene, phellandrone, ι-limonene, β-eudesmol, δ -cadinone, elemol, trans-caryophyllene으로 동정되었으며 그 중에서도 phellandrene, β-eudesmol이 주된 역할을 하는 성분으로 확인하였다. The composition and chemical structures of same individual components of essential oils from ginger flavor plants were estimated by gas chromatography and gas chromatography-mass spetrometric analysis with the aid of NBS and Wiley library and RI indice searches. Through gas chromatography and gas chromatography /mass spetrometry analysis of 43, 41, 32 essential oil components from flowers, leaves and stems from Lindera obstusiloba., respectively were identified, among which sabinene, β -myrcene, ι-limonene, phelandrene, ~gamma -selinene, α -terpinene, 2, 4a, 5, 6, 7, 8, 9, 9a -octahydro benzocycloheptane, δ -cadinene, ~gamma -terpinene, (Z) -3-hexen-1-ol acetate, ~gamma -elemene, l-boreneol, δ -guaiene, ledene, cis-3-hexanal, elemol, α -chamigrene, β -endesmol: 9-octadecanal, 1-(1, 5-diMe-4-hexenyl)-4-Me. benzene were estimated to be major components.

시라카바파(白樺派)의 서양미술 수용과정 -『시라카바(白樺)』와 「니카회(二科会)」를 중심으로-

문형자(文亨子) 한국일본문화학회 2020 日本文化學報 Vol.0 No.86

This study examines the effects of the Shirakaba school’s acceptance of Western art with a focus on the “Shirakaba” and "Nika" groups. Since the Meiji period, Japan has implemented a national policy aimed at catching up with Western advancements and there has been a movement to reflect this trend in the art field. In the early Meiji period, the promotion of art grew under the protection of the government as part of national policy. The Meiji government established an art school and a government-sponsored exhibition to implement policies. However, there has been a series of abuse aimed at exhibition judges, undermining their authority and the nature of the policy that tried to bring advanced art into the system. Seiki Kuroda, a teacher at the Tokyo art school and then head of the Hakuba society attempted to overcome this situation. Shirakaba school formed the Nika group, insisting on seeing anti-academy and anti-exhibition participation. In the end, the Shirakaba school and Shirakaba magazine served as spearheads to promote Western art in Japan in the course of Japan’s modern westernization as well as stepping stones to nurture students after school. Meanwhile, as Kojima Kikuo and Hosokawa Moritatsu who were involved in the Shirakaba school had a certain status in the Western art troupe of Japan, intervened in the production of the portrait of Yasui Sotaro. Finally, the Shirakaba school has led to excessive interference in the creative activities of their successors.

톱풀의 항산화 성분

문형인,류승효,노종화,지옥표 한국약용작물학회 2000 한국약용작물학회지 Vol.8 No.1

톱풀 Achillea sibirica Ledeb. (Compositae: 국화과)은 국내 산지의 초원에 널리 분포하는 흰색꽃이 피는 다년생 초본으로 민간에서 봄의 어린 순을 나물로 먹고 전초와 종자를 말린 것(신초;神草) 을 , 건위(健胃), 강장(彈壯), 해열(解熱), 진경(鎭湮), 진통(鎭痛), 정기증진 (精氣增進), 출혈이 심한 치질에 사용해 왔다. 천연물로부터의 우수한 항산화제의 개발의 일환으로 이 식물의 전초의 MeOH extract와 그것의 Hexane fraction., Methyene Chloride fraction., Ethyl Acetate fraction, Butanol fration 에 대해 DPPH radical 소거 효과에 의한 검색을 실시한 결과 EtOAc 분획에서 우수한 항산화 활성을 나타내어 그 EtOAc 용매분획층을 column chromatography 하여 2종의 flavonoid glycoside (E-1,2) 를 분리하였으며, hexane분획에서의 1종의 sterol 화합물 (H-1)과 더불어 각종 이화학적 성상과 spectral data로부터 구조를 동정한 결과 각각 sterol mixture (H-1), kaempferol-3-O-glucoside(E-1), luteolin-7-O- neohesperidoside(E-2)로 확정하였고, 그 중 화합 H-1은 campesterol, stigmasterol, β-sitosterol이 혼합되어 있음을 확인하였다. 화합물 E-1, 2에 대해 항산화 활성 검색을 실시하여, DPPH radical 소거 효과에 의한 검색에서 각각 12.5μg/ml과 15.4μg/ml에서 EC50을 나타내어 항산화 효과를 보였고, TBA에 의한 비색정량법에 의한 항산화 활성 검색에도 항산화 효과를 보였다. Achillea sibirica Ledeb. is widely distributed in Korea and has been often used as folk medicine in peptic and tonic. As one of our searches for bioactive (anti oxidation) compounds from medicinal plants, we studied Achillea sibirica Ledeb. (Compositae). Antioxidant activity of Achillea sibirica was determined by measuring lipid peroxide produced when a mouse liver homogenate was exposed at 90℃ using 2-thiobarbituric acid (TBA) and by evaluation the radical scavenging activity on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical. Whole parts of Achillea sibirica was extracted with methanol and its extracts was fractionated with organic solvent; n-Hexane, methylene chloride, ethyl acetate, n-Butanol. EtOAc fraction exhibited antioxidant activity and From its, two flavonoid glycosides were isolated by silica gel and gel filtration colume chromatography and identified to kampferol 3-O-glucoside and luteolin 7-O-neo-hesperidoside, respectively, by physico-chemical and spectroscopical method. At antioxidant activity test for two compounds isolated, antioxidant activity was showed too. And from hexane fraction sterol was is isolated and identificated to mixture of campesterol, stigmasterol, and β-sitosterol.

백서에서 Depulpin^®과 Formocresol에 대한 치수와 치근단 조직의 반응

문형인,김선호,황윤찬,오병주,황인남,김선헌,정선와,윤창,오원만,Moon, Hyung-In,Kim, Sun-Ho,Hwang, Yun-Chan,Oh, Byung-Ju,Hwang, In-Nam,Kim, Sun-Hun,Jeong, Sun-Wa,Youn, Chang,Oh, Won-Mann 대한치과보존학회 2002 Restorative Dentistry & Endodontics Vol.27 No.4

One fifth dilution of formocresol is usually used for pulpotomy of the primary teeth and emergency pulpotomy of the permanent teeth. However the use of formaldehyde has been subjected to criticism because it may be absorbed into the blood stream and become distributed systemically, it nay also alter the pulp tissue rendering it immunologically active, and have carcinogenic potential. Recently Depulpin$^{\circledR}$(VoCo., Germany) gains popularity as a devitalizing agent during root canal therapy in spite of high concentration of 49 % paraformaldehyde because it facilitate devitalization of pulp and make root canal therapy easier But there have been not enough publications about the reaction of pulp and periapical tissue caused by Depulpin. This study was performed to evaluate the histological changes in pulp and periapical tissue of rats after pulpotomy using formocresol and Depulpin and to elucidate the toxic effects of these agents. Thirty six Sprague-Dawley rats were anesthetized by intraperitoneal injection of ketamine Maxillary first molar teeth were used for pulpotomy with formocresol and Depulpin. Rats were sacrificed after 2 days, 4 days, 1 week, 2 weeks, 3 weeks and 4 weeks respectively. Specimens were histologically observed by light microscope changes in pulp and periapical tissue. The obtained results were as follows. 1. Formocresol group A zone of fixed tissue. in which odontoblasts could clearly be defined, was present directly underneath the pulpotomy dressing in almost all teeth of this group. This was followed by an area of necrotic tissue which resembled dried out fibrous tissue with no cellular detail except some pyknotic nuclei. In the specimens of after 2 days, 4 days, 1 week, 2 weeks in which vital tissue was present, it was separated from the fibrous area by a zone of inflammation. In the specimens of after 3 weeks and after 4 weeks, inflammatory infiltrate was in the periodontal ligament adjacent to the apical foramina of the teeth. 2. Depulpin$^{\circledR}$ group The area of necrotic tissue which had no cells and fibers, was present adjacent to the dressing. This was followed by dried out fibrous tissue with no cellular details except some pyknotic nuclei, A short stump of vital pulp with odontoblasts was present at the end of the canal after 2 days. Inflammatory infiltrate was in the periodontal ligament after 4 days and after 1week. Severe root resolution and necrosis of periapical tissue opposite the root resorption site were defined after 2 weeks and after 3 weeks. Periapical lesion which consist of necrotic tissue surrounded by a fibrous connective wall, was found after 4 weeks. The results indicated that Depulpin can cause more adverse reaction to the dental pulp and periapical tissue than formocresol, and further studies are needed for its clinical use with safety.

전자코 시스템에 의한 향미의 방향미 성분 분석

문형인,이재학,이동진 韓國作物學會 1996 Korean journal of crop science Vol.41 No.6

전자코를 이용하여 향미품종에 대한 방향성 성분함량비율을 조사한 결과를 요약하면 다음과 같다. 1. 작용기별로는 polar compound계열과 aldehydes compounds계열이 apolar compounds 계열과 sulphur compounds, aminated com-pounds계열에 비해 약 3배정도 높은 함량 비율을 보였다. 2. 백미와 취반상태에 있어서 방향성 성분 함량변화는 대부분 0.02에서 0.1수준으로 증가하는 경향을 보였으나, 찰성을 가진 밀양 14006의 경우는 취반 상태에서 방향성 성분의 함량이 오히려 감소하는 경향을 보였다. 3. 향기와 amylose함량 관계에서는 Japonica와 Indica계열 모두 아밀로스함량이 높을수록 방향성 성분의 함량이 증가하는 경향을 보였다. 4. Headspace 진행 후 센서에 감지되는 시간대는 대부분의 품종에서 30~35초에서 가장 많은 향기성분의 양을 검출할 수 있었으나, 이리 41003의 경우는 방향성성분의 검출이 30초대 이후에 급격히 감소하였다. Volatile flavour components rates from aromatic rices were analyzed by Electronic nose systems. In functional group, polar compounds and aldehyde compounds showed much of volatile flavour components than apolar compounds, sulphur compounds and aminated compounds. The profiles of volatile flavour components rates were markedly differents of sen-sing times, amylose content.