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연속 발효조를 이용한 soluble glucan 생산 공정 개발
문찬준,이중헌,Moon, Chan-Jun,Lee, Jung-Heon 한국생명과학회 2006 생명과학회지 Vol.16 No.6
Continuous fermentation process for the production of soluble glucan using mutant Agrobacterium sp. ATCC31750 has been developed in this research. When the concentration of soluble glucan was higher than 6 g/l, the viscosity of the fermented broth was too high and it needs complex separation process to separate from culture broth. Mathematical models which describe the cell growth and glucan production was developed and they kinetic parameters were estimated with experimental data. They are used for the optimization of continuous fermentation process and calculate optimal dilution rate for easy separation of glucan 4 g/l. With continuous fermentation, glucan production rate was increased 1.8 times more than that with batch fermentation.
Agrobacterium sp.를 이용한 바이오폴리머 생산에서 발효조건들의 영향
문찬준,이중헌 조선대학교 생산기술연구소 2007 生産技術硏究 Vol.29 No.2
Effects of carbon, nitorgen, PH, and reactor types on biopolymer production with Agro bactehum sp. were studied in this article. The optimal glucose concentration for cell gro wth was 40 g/l and that for pH was 6. The optimal pH for glucan production was 8. Fo r the two different types of fermentors, stirred tank and bubble column, the biopolymer p roduction was examined. The bubble column fermentor which has high oxygen transfer rate showed the high biopolymer production characteristics.