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김가은,김관우,김동교,김승창,조해열,이은도,정상욱,문성실,이진욱,김찬란,최봉환 한국산학기술학회 2023 한국산학기술학회논문지 Vol.24 No.11
The purpose of this study was to develop individual goat identification and paternity DNA testing methods to eradicate the illegal distribution of goats and goat meat and maximize the effects of goat improvement through scientific pedigree management. To reduce goat allele discrimination errors and increase the reliability and accuracy of the analysis, 15 new goat individual identification genetic markers consisting of 3- and 4-repeat microsatellite loci (KCH004, KCH006, KCH015 KCH027, KCH047, KCH067, KCH086, KCH114), KCH117, KCH121, KCH122, KCH134, KCH153, KCH165, KCH212) were developed. As a result of performing muliplex polymerase chain reaction (PCR) microsatellite genotyping on 96 goats using the selected 15 loci, a total of 54 alleles were found, and it was confirmed that each locus had an average of 3.60 alleles. The polymorphism information content (PIC) value, which is a measure of polymorphism and information power of markers, ranged from 0.090 (KCH153) to 0.545 (KCH121), and loci KCH006, KCH015, KCH114, and KCH121 had a PIC of 0.35 or higher. The average PIC value of all 15 markers was confirmed to be 0.28, and it was verified that there was a polymorphism as an individual identification marker. Using the developed markers, the genetic characteristics of native black goats (Dangjin, Jangsu, and Tongyeong lines) and five foreign breeds (Alpine, Boer, Nubian, Saanen, and Toggenburg) were analyzed. The heterozygosity of the genetic markers was measured. The expected heterozygous ratio was found to be within the range of 0.167 (Nubian) to 0.467 (Boer), and the native black goat had values of 0.393 (Dangjin), 0.415 (Tongyeong), and 0.437 (Jangsu). The analysis of the affinity relationship between native black goats (Dangjin, Jangsu, and Tongyeong) and the five foreign breeds was performed by estimating the genetic distance based on specific allele frequencies. It was found that the genetic distance between the native black goat and the Boer population was the closest and it was farthest from Saanen. It is believed that the 15 markers developed by confirming the genetic differentiation patterns of goats can be used not only for individual identification and paternity genetic identification but also for goat genetic diversity and related relationship analysis.
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남북한 초ㆍ중ㆍ고등학교 국어교육 통합을 위한 기초 연구 : 통일 대비 단계의 국어과 교수·학습 내용 선정 및 수업 분석을 중심으로
고정희,김종철,구본관,조진수,차경미,민병곤,위현길,윤구희,이은정,김호태,문찬란,김진아,심미진 서울대학교 국어교육연구소 2015 국어교육연구 Vol.36 No.-
이 연구는 통일 대비 단계에서 남북한 학습자에게 필요한 교육 내용 및 방법을 개발하고, 이를 실제 현장에서 수행해 봄으로써 통일 이후 국어교육 통합에 대한 지침을 제공하는 것을 목적으로 수행되었다. 이를 위해 남북한 교육과정과 교과서, 북한 이탈 청소년을 위한 교육과정 및 교과서를 분석하고, 북한 학습자에 대한 이해를 도모하기 위해 북한 이탈 청소년 대안 학교를 참관하였다. 이를 바탕으로 통일 대비 남북한 학습자를 위한 교육 내용을 선정하여 교수‧학습 과정안을 구안하였다. 구안된 교수학습 과정안을 활용하여 학교 현장에서 실제 수업을 실시하고, 수업 장면을 동영상으로 촬영하여 학습자 반응을 분석하였다. 마지막으로 현직 교사를 대상으로 전문가 수업 평가를 실시하였다. 이상의 내용을 바탕으로 본고에서는 크게 ‘언어적 차이 및 국어의식의 차이 인식 측면’, ‘통일 대비 국어교육 통합을 위한 수업 주제 및 제재의 적절성 측면’, ‘학습자의 수준 고려 측면’에서 수업을 분석하여 향후 연구를 위한 제언을 마련하였다. This study aims to develop Korean language educational contents and methods, which is necessary in preparation the unification of Korea. These contents and methods, will then be applied to a classroom setting and guidelines for unifying Korean language education in South and North Korea will be Provided. To develop educational contents and methods, we analyzed the curriculum and textbooks of South and North Korea, and those for North Korean youth refugees. In addition, we observed classes in alternative schools for North Korean youth refugees. We created teaching-learning lesson plans, applied those to a classroom setting, surveyed the students, and requested current teachers to evaluate the class. Based on the above information, we analyzed the lessons according to the following points: a consciousness of language differences and differences in language consciousness, appropriateness of class topics and teaching materials, and consideration of the learner’s ability. This helped us prepare recommendations for future research.
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