위정맥류 출혈과 비장경색을 합병한 만성췌장염 1예

문윤기,이보인,강성호,양영상,차현석,최규용,정인식,차상복,박두호 大韓消化器病學會 1995 大韓消化器病學會誌 Vol.27 No.5

호박 흰가루병의 피해 해석 및 경제적 방제수준 설정

문윤기,최준근,강안석 한국식물병리학회 2010 식물병연구 Vol.16 No.3

The experiments were carried out in fields for two years from 2008 to assess yield losses of squash due to powdery mildew caused by Sphaerotheca fuliginea and to determine its economic thresholds. Powdery mildew disease was first observed in late June, about 50 days after field-transplanting, progressed rapidly during late July to early August, and began to reduce from late August. Powdery mildew severity was negatively correlated with squash yields. A positive correlation was observed between fruit weight and %marketable fruits. A simple linear regression model was obtained as Y=−10.399 X + 6607.5 with R2= 0.9700when squash yields (Y) was predicted using powdery mildew severity as an independent variable(X). Spray threshold for maximizing squash yields without economic considerations was estimated as 6.5% in terms of leaf lesion area with powdery mildew. Economic threshold and economic spray threshold able to compensate the costs of fungicide sprays were determined as 21.6% and 17.3% in leaf lesion area, respectively. 호박 흰가루병의 수량감수 모델, 요방제 수준 및 경제적 방제수준을 산출하기 위하여 2008년부터 2년간 포장에서 시험하였다. 호박 흰가루병은 정식 후 50일경인 6월 하순에 발생하기 시작하여 7월 하순~8월 상순에 가장 심하였고, 그 이후 감소하는 경향을 보였다. 흰가루병의 발병정도와 수확 과실수, 과실의 생체중, 수량(생체중×수확 과실수)간에는 고도의 부의 상관이 있었고 생체중과 상품과율은 정의 상관을 보였다. 흰가루병의 발병수준(X)에 따른 호박의 수량(Y) 추정 단순직선회귀식은 Y = −10.399X + 6607.5였고 결정계수(R2)는 0.9700으로 모델의 적합도가 매우 높았다. 방제비용을 고려하지 않은 흰가루병의 요방제 수준은 병반면적율 6.5%였으며, 방제비용을 정당화 시킬 수 있는 경제적 피해수준은 병반면적율 21.6%였고, 농가에서 실제로 적용 가능한 경제적 방제수준은 병반면적율 17.3%로 산출되었다.

환자의 행동과 인지를 통해서 본 병원 외래의 고찰 : 병원 지리학에 관한 연구 1 Stuies On Hospital Geography 1

문윤기 한국병원건축학회 1996 의료·복지 건축 Vol.2 No.3

환자의 의식과 행동의 경일적 변화에서 본 입원환경의 태도에 대한 연구 : 병원 지리학에 관한 연구 2 Stuies On Hospital Geography 2

문윤기 한국병원건축학회 1996 의료·복지 건축 Vol.2 No.3

종합병원의 건축 프로그래밍을 위한 전문가 시스템 개발에 관한 연구

문윤기,이낙운 한국의료복지시설학회 1997 의료·복지 건축 Vol.3 No.5

The purpose of this thesis is to develop the new method of the expert system as a new approach of the architectural programming in general hospital. Theoretical and field investigations have been made for this study, and finally, the computer pro-gram for the architectural programming of general hospital was established. The architectural programming of general hospital is more complex than any other types of buildings and has been changed rapidly according to the social, cultural and environmental factors. Hence, a new approach method is necessary for corresponding to the complexity and these factors. Therefore, the computer should be used as a tool in order to operate more logical and systematic programming.

Damping-off of Edible Aster Caused by Rhizoctonia solani AG-4

문윤기,Se-Won Kim,Ki Jin Park,김완규 한국식물병리학회 2022 식물병연구 Vol.28 No.4

In August 2021, we surveyed diseases of wild vegetables grown in Taebaek, Gangwon Province, Korea. During the disease survey, we observed severe damping-off symptoms in young edible aster (Aster scaber) plants in a vinyl greenhouse investigated. The incidence of the disease in the plants ranged from 5% to 20%. Diseased plants of edible aster were collected from the vinyl greenhouse, and fungi were isolated from petiole lesions of the diseased plants. Rhizoctonia sp. was consistently isolated from the petiole lesions. We examined morphological characteristics and anastomosis groups of nine Rhizoctonia sp. isolates obtained from the petiole lesions. The examination results revealed that all the isolates corresponded to Rhizoctonia solani AG-4 based on the morphological characteristics and anastomosis test. Three isolates of R. solani AG-4 were tested for their pathogenicity on edible aster plants by artificial inoculation. Inoculation tests showed that the tested isolates caused damping-off symptoms on the inoculated plants. The induced symptoms were similar to those observed in the vinyl greenhouse investigated. Damping-off of edible aster caused by R. solani AG-4 is first reported in this study.

전신성 홍반성 낭창에서 바생한 관상동맥의 조기 죽상경화증 1예

문윤기,김용주,전두수,강동헌,이만영,승기배,채장성,김호연,김재형 대한순환기학회 1995 Korean Circulation Journal Vol.25 No.3

Sclerotium rolfsii에 의한 곰취 흰비단병

문윤기,김세원,최준근,심홍식,주호종,최인영 한국식물병리학회 2015 식물병연구 Vol.21 No.1

In June 2012 and 2013, a destructive stem rot symptoms of Ligularia fischeri occurred sporadically in Hoengseong-gun and Pyeongchang-gun Gangwon-do, Korea. The typical symptom included water-soaking onthe main stem, rotting, wilting and blighting, which eventually leads to death of the plant. White mycelialmats were spread over lesions and brown sclerotia were formed on stems and near soil surface. The sclerotiawere white to brown, spherical or irregular, 1-3 mm in size on potato dextrose agar (PDA), The optimumtemperature range of hyphal growth was 25-30oC and the hyphal diameter was 4-10 mm. The typical clampconnections were observed in the hyphae of the fungus grown on PDA. The resulting sequence of 695 bpwas deposited in GenBank. A BLAST search revealed that sequences of the this isolates showed >99% identitywith those of Sclerotium rolfsii. On the basis of the morphological characteristics and phylogenetic analyses ofmolecular markers ITS rDNA, the fungi were identified as S. rolfsii. A pathogenicity test was carried out to fulfillKoch’s postulates. To our knowledge, this is the first report of S. rolfsii on Ligularia fischeri in Korea. 2012년과 2013년 6월 하순 강원도 횡성과 평창의 곰취(Ligularia fischeri) 재배포장에서 포기가 서서히 시들어 말라 죽는 이상 증상이 발생하였다. 발병정도는 30-80%로 병든 식물체를 조사한 결과, 줄기가 수침상으로 물러지고 썩으면서 흰색의 곰팡이와 갈색의 작고 둥근 균핵이 관찰되었다. 병원균을 순수 분리하여 병원균의 균학적 특징을 조사한 결과, 감자한천배지에서 균총은 흰색으로 잘 자라며 갈색의 작고 둥근 균핵을 많이 형성하였다. 균핵의 크기는 1-3 mm이며, 균사의 폭은 4-10 mm였다. 균사생육과 균핵 형성 적온은 25-30oC이었으며, 균사특유의 clamp connection이 관찰되었다. 또한, 병원균의 염기서열분석결과 695 bp로 Sclerotium rolfsii와 같은 계통군으로 확인되었으며, 99% 이상의 상동성을 보였다. 이와 같이 곰취에 발생한병징, 병원균의 균학적 특징 및 염기서열 분석 등을 종합한 결과 S. rolfsii Saccardo로 동정되어 곰취 흰비단병으로 명명하고자 한다.

Shoot Rot of Spikenard Caused by Rhizoctonia solani AG-2-1

문윤기,서현택,박기진,김완규 한국식물병리학회 2022 식물병연구 Vol.28 No.1

In January 2021, unusual outbreak of shoot rot symptoms was observed in young spikenard (Aralia cordata) plants growing in vinyl greenhouses located in Chuncheon and Yanggu, Gangwon Province, Korea. The symptoms initially appeared on young shoots of the plants at or above the soil surface level. Later, the infected shoots wholly rotted and blighted. The incidence of diseased plants in the vinyl greenhouses investigated ranged from 5% to 30%. Eight isolates of Rhizoctonia sp. were obtained from shoot lesions of the diseased plants. All the isolates were identified as Rhizoctonia solani AG-2-1 based on the morphological characteristics and anastomosis test. Three isolates of R. solani AG-2-1 were tested for pathogenicity on young shoots of spikenard plants using artificial inoculation. All the tested isolates induced shoot rot symptoms on the inoculated plants. The symptoms were similar to those observed in spikenard plants from the vinyl greenhouses investigated. This is the first report of R. solani AG-2-1 causing shoot rot in spikenard.

십자화과 채소작물에서의 후사리움 시들음병 발생과 그 원인균의 병원성 분화

문윤기,김완규,조원대,성재모 한국식물병리학회 2001 식물병연구 Vol.7 No.2

1996년부터 1998년까지 한국내 십자화과 채소작물의 포장에서 시들음병 발생을 조사한 결과, 배추와 무에서는 발생율이 최대 40%까지 심하게 발생하였으며, 양배추에서는 일부 포장에서만 약간 발생하였다. 시들음증상을 보이는 십자화과 채소작물의 병든 뿌리로부터 총 123균주의 Fusarium균이 분리하였으며, 공시균주들의 형태적 및 배양적 특성을 조사한 결과, 모두 Fusarium of oxysporum으로 동정되었다 공시균주들의 배추 및 양배추에 대한 병원성은 공시품종에 따라 다소 차이는 있었으나 균주들간에 뚜렷하게 구분되지 않았다. 무에 대한 병원성에 있어서는 대체로 무분리 균주들이 배추 및 양배추분리 균주들보다 강한 것으로 나타났다 공시균주들의 십자화과 이외의 채소작물에 대한 병원성검정 결과, 고추 등 8개 작물에 대해 모두 병원성이 없었다 이 연구결과, 배추감염 균주들의 병원형은 양배추감염 균주들의 병원형과 동일하고, 무감염 균주들의 병원형과는 다른 것으로 나타났다. Occurrence of Fusarium wilt was surveyed in fields of cruciferous vegetable crops in Korea from 1996 to 1998. The disease severely occurred up to 40% in fields of Chinese cabbage and radish but slightly in Fields of cabbage. A total of 123 isolates was obtained from roots of the diseased plants and identified as Fusarium oxysporum based on the morphological and cultural characteristics. Pathogenicity of nine isolates selected from the isolates was tested by artificial inoculation to the hosts. All the isolates had similar virulence on Chinese cabbage and cabbage, although there were some differences in virulence on cultivars tested among the isolates. The isolates from radish were more virulent to radish than those from Chinese cabbage and cabbage. All isolates from the crucifers were not virulent to eight species of vegetable crops except the crucifers. The results of pathogenicity tests showed that the pathotype of Chinese cabbage-infecting isolates was identical to that of cabbage-infecting isolates, but somewhat different from that of radish-infecting isolates.