수종 담수적조 원인종들의 형광특성과 적용연구

손문호,권오섭,문병용,정익교,이춘환,이진애,Son, Moon-Ho,Zulfugarov, Ismayil S.,Kwon, O-Seob,Moon, Byoung-Young,Chung, Ik-Kyo,Lee, Choon-Hwan,Lee, Jin-Ae 한국조류학회(藻類) 2005 ALGAE Vol.20 No.2

The freshwater blooms mainly blue-green algal blooms occur frequently in the lower Naktong River in summer, which provoke many socio-economical problems; therefore, the early detection of bloom events are demanding through the quantitative and qualitative analyses of blue green algal species. The in vivo fluorescence properties of cultured strains of Microcystis aeruginosa, M. viridis, M. wesenbergii, M. ichthyoblabe, Anabaena cylindrica, A. flos-aquae, and Synedra sp. were investigated. Wild phytoplankton communities of the lower Naktong River were also monitored at four stations in terms of their standing stocks, biomass and fluorescence properties compared with its absorption spectram. The 77K fluorescence emission spectra of each cultured strains normalized at 620 nm was very specific and enabled to detect of blue green algal biomass qualitatively and quantitatively. The relative chlorophyll a concentration determined by chlorophyll fluorescence analysis method showed significant relationship with chlorophyll a concentration determined by solvent extraction method ($R^2$ = 0.906), and the blue-green algal cell number determined by microscopic observation ($R^2$ = 0.588), which gives insight into applications to early detection of blue green algal bloom.

낙동강 하구 생태계 식물성 플랑크톤의 일차생산성

이진애,조경제,권오섭,정익교,문병용 ( Jin Ae Lee,Kyung Je Cho,O Seob Kwon,Ik Kyo Chung Byung Yong Moon ) 한국하천호수학회 1994 생태와 환경 Vol.27 No.1

Primary production of phrtoplankton was investigated in Naktong estuarine ecosystem monthly from October 1991 to September 1992 using carbon-14 uptake method. In spite of large spatial and temporal variation, there was a primary peak of net phytoplankton production in spring through early summer. The productivity was minimum of 543mg C·m^-2.day^-1 in September and maximum of 4112mg C·m^-2·day^-1 in April. The seasonal changes in factors regulating production in Naktong estuarine area were proposed based on the annual patterns of physical variables and nutrient concentrations. The late spring and early summer flowering of biomass and net production of phytoplankton was explained as the result of the balance between light and temperature. The high level of Production terminated in summer as the result of water clarity. Nutrient limitation may not be important, because of high nutrient level year around in this eutrophicated estuarine area.

도시근교 하천 조만강의 (潮滿江) 식물플랑크톤 일생산성 추정

조경제,신재기,이진애,문병용 ( Kyung Je Cho,Jae Gi Shin,Jin Ae Lee,Byeong Yong Moon ) 한국하천호수학회 1995 생태와 환경 Vol.28 No.1

Hourly photosynthetic P-I curves were used to estimate the daily production from in situ C-14 data. P^B_(max) were ranged from 1.4 to 7.5(㎍ C/㎍ chl-a/hr) and α ranged from 3.6 to 14.8[㎍ C/㎍ chl-a/(mol/m^2/hr)]. P^B_(max) and α were simultaneously high in the growing season and low in winter. α and I_k were good indicators for seasonally low-irradiance adaptation in algal photosynthesis. At surface high irradiance, photoinhibition didn`t occur and algal assimilation showed a linear or logarithmic decrease along the depth. As water transparency dropped, primary productivity per unit area didn`t increase as production per unit volume did at water surface. Daily productions were estimated from 1.5(g C/m^2/day) at January to 4.0 (g C/m^2/day) at July. The photoadaptation and lack of photoinhibition were reflected by the role of irradiance and water temperature as most significant predictors of daily algal production. Carbon contents of phytoplankton comprised of 33% of particulate organic carbon of water, turn-over rate (P/B) of phytoplankton was 0.61/day and turn-over time was 1.6 day. Choman River, a stagnant and fertilized river near Kimhae city, is characterized by high nutrient loading from the surrounding area and high autochthonous production.

보리 유식물에 처리한 수은의 분포 및 독성 연구

이춘환,장호식,정익교,전현식,이진범,문병용,이진애 한국환경과학회 1992 한국환경과학회지 Vol.1 No.1

The inhibitory effects of mercury ions on the growth of barley seedlings were studied and the distribution of metal elements in the organs of treated plants was investigated by using synchrotron radiation induced X-ray emission (SRIXE). Although the treatment of mercury ions caused growth inhibition, the mercury-specific increase in variable fluorescence and the abolishment of energy-dependent quenching in broken barley chloroplasts as shown by Moon et al. (1992) were not observed in the leaves of growth-inhibited seedlings. Instead the treatment of mercury decreased Fmax and Fo values. However, Fmax/Fo ratio and photochemical and nonphotochemical quenching coefficients were not affected significantly. By SRIXE analysis of 10μM mercury chloride treated seedlings, accumulation of mercury in roots was observed after 1 hour of treatment and similar concentration was sustained for 48 hours. Relative contents of mercury was high in roots and underground nodes where seeds were attached, but was very low in leaves. Iron and zinc were also distributed mainly in the lower parts of the seedlings. However after 72 hours of treatment the contents of these metals in roots decreased and their distribution became more uniform, which may lead to death of the plants. These results suggest that the observed inhibitory effects on barley seedlings upto 48 hours after the treatment is not due to direct damages in the photosynthetic apparatus, but due to its accumulation in roots and the consequent retardation of the growth of barley seedlings. The decrease in Fmax and Fo is probably due to the decrease in chlorophyll and protein contents caused by the retardation of growth. The observed slow expansion of primary leaves could be also explained by the retardation of growth, but the fluorescence induction pattern from the leaves did not show characteristic symptoms of leaves under water stress.

보리 유식물에 처리한 수은의 분포 및 독성 연구

이춘환,이진범,장호식,문병용,정익교,전현식,이진애 동의대학교 기초과학연구소 1993 基礎科學硏究論文集 Vol.3 No.1

The inhibitory effects of mercury ions on the growth of barley seedlings were studied and the distribution of metal elements in the organs of treated plants was investigated by using synchrotron radiation induced X-ray emission (SRIXE). Although the treatment of mercury ions caused growth inhibition, the mercury-specific increase in variable fluorescence and the abolishment of energy-dependent quenching in broken barley chloroplasts as shown by Moon et al.(1992) were not observed in the leaves of growth-inhibited seedlings. Instead the treatment of mercury decreased Fmax and Fo values. However, Fmax/Fo ratio and photochemical and nonphotochemical quenching coefficients were not affected significantly. By SRIXE analysis of 10μM mercury chloride treated seedlings, accumulation of mercury in roots was observed after 1 hour of treatment and similar concentration was sustained for 48 hours. Relative contents of mercury was high in roots and underground nodes where seeds were attached, but was very low in leaves. Iron and zinc were also distributed mainly in the lower parts of the seedlings. However after 72 hours of treatment the contents of these metals in roots decreased and their distribution became more uniform, which may lead to death of the plants. These results suggest that the observed inhibitory effects on barley seedlings upto 48 hours after the treatment is not due to direct damages in the photosynthetic apparatus, but due to its accumulation in roots and the consequent retardation of the growth of barley seedlings. The decrease in Fmax and Fo is probably due to the decrease in chlorophyll and protein contents caused by the retardation of growth. The observed slow expansion of primary leaves could be also explained by the retardation of growth, but the fluorescence induction pattern from the leaves did not show characteristic symptoms of leaves under water stress.