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넙치(Paralichthys olivaceus) 성어의 viral hemorrhagic septicemia (VHS) 진단사례
김진우 ( Jin Woo Kim ),조미영 ( Mi Young Cho ),이한나 ( Han Na Lee ),한현자 ( Hyun Ja Han ),이순정 ( Soon Jeong Lee ),지보영 ( Bo Toung Jee ),명정인 ( Jeong In Mteong ),원경미 ( Kyoung Mi Won ) 한국수산과학회 2012 한국수산과학회지 Vol.45 No.6
We examined the cause of a disease outbreak in adult olive f lounder Paralichthys olivaceus , which occurred at a Korean aquaculture farm in Korea in 2011. The principal signs included an expanded abdomen and congested liver, with persistent mortality (a little over two months). At the beginning of the outbreak, farm administrators misjudged the disease as bacterial in origin, because of the aforementioned signs, persistent mortality, and the detection of bacterial species, including Vibrio spp. and Streptococcus spp. Moreover, the detection of viral hemorrhagic septicemia virus (VHSV) by reverse trasnscription-PCR analysis was complicated by use of the VHS-VN primer set, which has been in general use recently, because it produced weak bands in some samples. Therefore, we recommend the use of at least two different primer sets in the diagnosis of VHSV. Our histopathological f indings indicate that necrotizing myocarditis could be considered a pathogenic sign of VHSV infection.