The Global Histone Modification Patterns of Osteosarcoma

도성임,임성직,김윤화,Liliana G. Olvi,Eduardo Santini-Araujo,박용구 대한병리학회 2011 Journal of Pathology and Translational Medicine Vol.45 No.2

Background: Epigenetic alteration may affect a patient’s prognosis by altering the development and progression of the tumor. Some recent reports have identified a correlation between histone modification and patient outcome. However, no studies have been conducted on global histone modification in osteosarcomas. Methods: We investigated histone modification in 54 cases of osteosarcoma by performing immunohistochemical staining. The immunohistochemical expres­sion of four histone modification markers, acetylated H4 lysine 12 (H4K12Ac), acetylated H3 ly­sine 18, trimethylated H3 lysine 27, and dimethylated H3 lysine 4 were evaluated. Results: High H4K12Ac expression was correlated with patient age (p=0.011). However, the other histone mod­ification markers showed no correlation with any of the clinicopathological data such as survival, tumor grade, tumor site, metastasis, age, or gender. Conclusions: Our study showed that all four histone modification markers are expressed in osteosarcoma (median expression rate, 40 to 60%). However, we did not find a correlation with the clinicopathological factors except for age. Further study to evaluate the reason for the association between H4K12Ac and patient age is needed.

Correlation Between Cyclooxygenase-2 Expression and HuR Cytoplasmic Translocation of Breast Cancer

도성임,도인구,김교영,이선,김윤화,박용구,이주희,임성직 대한병리학회 2008 Journal of Pathology and Translational Medicine Vol.42 No.2

Background : Embryonic lethal abnormal vision (ELAV)-like protein HuR is known to stabilize mRNA through binding AU-rich elements in the 3’-untranslated region. Recent studies show that HuR expression is associated with the expression of several genes including cyclooxygenase- 2 (COX-2). HuR exists predominantly in the nucleus, but cytoplasmic translocation of HuR is thought to be more important for its activity. COX-2 is a well-known enzyme that promotes tumor growth. Methods : To evaluate the correlation of HuR and COX-2 expression, we analyzed expression of HuR and COX-2 in 91 cases of breast cancer using immunohistochemistry. Results : Nuclear and cytoplasmic expression of HuR was seen in 76 (83.5%) and 19 (20.9%) of 91 cases respectively. COX-2 immunoreactivity was seen in 54 (59.4%) cases. Cytoplasmic HuR expression showed significant correlation with COX-2 expression (p=0.001). Nuclear HuR showed no correlation with COX-2 expression or other clinicopathological parameters. COX-2 expression is significantly associated with tumor grade (p=0.028). COX-2 (p=0.092) and cytoplasmic (p=0.569) and nuclear HuR (p=0.247) expression showed no correlation with survival. Conclusions : These results suggest that cytoplasmic HuR expression is associated with COX-2 expression in breast cancer and cytoplasmic location of HuR might contribute to the stabilization of COX-2 mRNA. Background : Embryonic lethal abnormal vision (ELAV)-like protein HuR is known to stabilize mRNA through binding AU-rich elements in the 3’-untranslated region. Recent studies show that HuR expression is associated with the expression of several genes including cyclooxygenase- 2 (COX-2). HuR exists predominantly in the nucleus, but cytoplasmic translocation of HuR is thought to be more important for its activity. COX-2 is a well-known enzyme that promotes tumor growth. Methods : To evaluate the correlation of HuR and COX-2 expression, we analyzed expression of HuR and COX-2 in 91 cases of breast cancer using immunohistochemistry. Results : Nuclear and cytoplasmic expression of HuR was seen in 76 (83.5%) and 19 (20.9%) of 91 cases respectively. COX-2 immunoreactivity was seen in 54 (59.4%) cases. Cytoplasmic HuR expression showed significant correlation with COX-2 expression (p=0.001). Nuclear HuR showed no correlation with COX-2 expression or other clinicopathological parameters. COX-2 expression is significantly associated with tumor grade (p=0.028). COX-2 (p=0.092) and cytoplasmic (p=0.569) and nuclear HuR (p=0.247) expression showed no correlation with survival. Conclusions : These results suggest that cytoplasmic HuR expression is associated with COX-2 expression in breast cancer and cytoplasmic location of HuR might contribute to the stabilization of COX-2 mRNA.

Associations between the Expression of Mucins (MUC1, MUC2, MUC5AC, and MUC6) and Clinicopathologic Parameters of Human Breast Ductal Carcinomas

도성임,김경은,김동훈,채승완,박용래,박찬흔,손진희 한국유방암학회 2013 Journal of breast cancer Vol.16 No.2

Purpose: Mucins are members of the glycoprotein family expressed in benign and malignant epithelial cells. The aim of this study is to evaluate the relationships between the expression of mucins in breast ductal carcinoma and clinicopathologic parameters. Methods: We constructed tumor microarrays based on 240 cases of invasive ductal carcinoma and 40 cases of ductal carcinoma in situ (DCIS) using formalin fixed, paraffin embedded tissues. We examined the expressions of MUC1, MUC2, MUC 5AC, and MUC6 by immunohistochemistry. Results: MUC1 demonstrated cytoplasmic, membranous, apical, and combinative expressions. Other mucins demonstrated cytoplasmic expression. In invasive ductal carcinoma, MUC1, MUC2, MUC5AC, and MUC6 were expressed in 93.6%, 6.2%, 4.8%, and 12.4% of cases, respectively; these rates were slightly, but not significantly, higher than observed in cases of DCIS. MUC1 expression was associated with estrogen receptor (ER) expression and negative MUC1 expression was associated with triple negativity. MUC6 expression was correlated with higher histologic grade, lymphatic invasion, lymph node metastasis, and HER2 positivity. No associations with any other clinicopathologic parameters were observed. Conclusion: Most invasive ductal carcinomas of the breast express MUC1, and this expression is associated with ER expression. MUC6 expression is correlated with some clinicopathologic parameters that are indicators of poor prognosis. To evaluate the role of MUC6 as a potential biomarker, further studies are warranted.

기본교육과정 체육1 교과서 내용 분석

도성화 ( Sung Wha Do ),전보성 ( Bo Sung Jeon ) 한국지적장애교육학회(구-한국정신지체아교육학회) 2010 지적장애연구 Vol.12 No.4

본 연구에서는 특수학교 기본교육과정 체육1 교과서를 분석하고 이를 토대로 체육 교과서의 구성 방안을 제시하고자 한다. 교과서는 체제와 내용의 두 가지 준거를 고려하여 분석하였으며 수집된 자료의 분석은 내용 분석법 (content analysis)을 사용하여 특수학교 체육 관련 전문가와 함께 분석하였다. 분석 결과는 첫째, 교과서 체제는 차례를 제외하고 178쪽으로 되어있으며 체조활동 32쪽 (18%), 육상 활동 36쪽(20.2%), 놀이·게임 및 스포츠 50쪽(28.1%), 표현 활동과 무용 20쪽(11.2%), 체력 운동 18쪽(10.1), 보건과 안전 22쪽(l2.4%)으로 구성되어 있었다. 둘째, 교과서 내용은 각 하위 내용 영역의 접근 방법에서 차이가 있었다. 학습 내용 전개에 있어서 영역 간 위계성이 부족하고 중복되는 내용이 있었다. 삽화와 사진은 명료하지 못하고 잘못 표현된 것이 있었다. 이런 내용들은 학생들에게 잘못된 정보를 제공하여 학습 결 과에 좋지 않은 영향을 미칠 수 있는 것으로 파악되었다. 내용 구성에 있어서는 학생들의 수준 보다 높은 내용이 제시 된 부분이 있었다. 이런 내용은 체육교육을 통한 학습목표 달성에 방해 요인이 될 수 있기 때문에 수정의 필요성이 있었다. This study is analyzed the Physical Education Text Book 1 of Basic Curriculum for Special School, which will form the basis through analysis and propose the component plan of The Physical Text Book 1. Text Book is analyzed considerable two standards of the system and contents, the collected date is applied a content analysis, and is analyzed by physical teachers and related experts together. The results of this study are as follows; First, the system of Text Book was composed with all of 178 pages except the content. Among them, there are gymnastics 32p.(18%), track and field 36p. (20.2%), playing games & sports 50p. (28.1%), expression activities & dance 20p. (11.2%), physical strength and athletic abilities 18p. (10.1%), and Health & safety 22p. (12.4%). Second, the contents of Text Book were difference of approach methods about each sub-contents domain. They were the lack of rank among areas and overlapped parts in the development of learning contents, and contained unclear illustrations and pictures, also wrong expressions. These might give wrong information to students, and might influence to the results of learning badly. We gave higher things than students level in the some components of contents. These things are needed the modification due to be able to obstructive factor for the achievement of learning object though Physical Education.

전자교과서를 이용한 지체부자유학교 초등부 교실 수업의 사회적 참여 구조 유형

도성화 대구대학교 특수교육재활과학연구소 2007 특수교육재활과학연구 Vol.46 No.3

The purpose of this study is to look into the types of social participation structure appeared in elementary classes with an electronic textbook for physical and multiple disabilities students. In order to achieve this goal, I participated and observed some special classes in the physical and multiple disabilities school. I observed 6th grade classes during 6 month period intensively and carried out in-depth interviews with teachers, parents and students. All instructions and contents of interviews was taped record and written. The result of this study came out several types that interaction between teachers and students depended on the student's character and the contents of classes in the physical and multiple disabilities school. First, all students and teacher together took a look and listened the explanation of an Electronic Textbook and then teacher dilated to all of them. Second, all students and teachers together took a look and listened the explanation of an Electronic Textbook, and then teacher dilated to some of them. After that, all of them would have individual homework. Third, teacher explained the contents of textbooks above all, and then all students listened an example of an Electronic Textbook. Forth, An Electronic Textbook explained questions above all, and then all students answered to the questions of teacher. Fifth, only some students answered to the questions of an Electronic Textbook Sixth, a student called on teacher listened and answered the question of an Electronic Textbook. Seventh, all of them listened the explanation of an Electronic Textbook and students asked and teacher answered. Eighth, all of them listened the explanation of an Electronic Textbook, and students discussed one another. Ninth, all of them listened the explanation of an Electronic Textbook, and students discussed one another about teacher's dilation. Sometimes teacher joined and discussed. According to this result, there appeared the types of SPS-1, SPS-2, SPS-4 interaction a lot in the physical and multiple disabilities school.

반응염료의 화학구조가 견직물의 염색성에 미치는 영향

도성국 東亞大學校 1998 東亞論叢 Vol.35 No.-

The dyeabilities of C. I. Reactive Blue 240 (R240, MW; 963.5) and C. I. Reactive Red 241 (R241, MW; 1135.5) were investigated. Initial dyeing rates were increased and the amount of dye on the fabric at equilibrium was decreased with temperature like other ordinary dyeing processes. Activation entropy(△S) was somewhat decreased because of loose bonding between dyestuffs and fiber molecules at transition state. It can be clarified that the entire reaction is exothermic and the number of molecular species at transition state becomes greater from decrease in activation enthalpy(△H) and the increase in activation free energy(△G) with temperature, respectively. Because of the bigger size of the dye molecule, the reaction rate of R241 was slower than that of R240.

소량의 학습 데이터에 대한 CNN 분류 성능 향상 방법

도성구(Seong-Gu Do),김민수(Min-Su Kim),이종혁(Jong-Hyeok Lee),김형준(Hyung-Jun Kim),김성현(Sung-Hyeon Kim),김상훈(Sang-Hun Kim),김영민(Yeong-Min Kim),박정훈(Park Jeong-Hun),양연모(Yeon-Mo Yang) 대한전기학회 2021 정보 및 제어 심포지엄 논문집 Vol.2021 No.4

골 견인술에 의한 경추골절 탈구환자의 치료에 대한 임상적 연구

도성신,안욱,신호 中央醫學社 1983 中央醫學 Vol.45 No.3

For the patient with fracture dislocation of the cervical spine, skeletal traction method is simple, safe and effective way to decompress and protect the spinal cord reestablishing the normal alignment of the spine. Skeletal traction is indicated as the initial treatment for the reduction of cervical fracture dislocations, and when properly applied, this method eliminate the necessity of laminectomy, spinal fusion, and the use of plaster supports in almost all cases of injury to this part of the spine. The results were satisfactory in most of our patients and the incidence of com-plication was negligiable or minor. The technique for correct application is briefly described.

[취재후기] 우리 모두는 늙는다···소풍 같은 인생, 그 쓸쓸함에 관하여

도성진 (사)방송기자연합회 2014 방송기자상 수상집 Vol.- No.-

의성지역에 분포하는 백악기 지층에 대한 고지자기 연구

도성재,김광호,Doh, Seong-Jae,Kim, Kwang-Ho 대한자원환경지질학회 1994 자원환경지질 Vol.27 No.3

Paleomagnetic and rock-magnetic data of Cretaceous sedimentary and volcanic rocks from the Euiseong area indicate that the stable components of remanence are carried by single and pseudo-single domain magnetite, with the exception of the Shinyangdong Formation which has been remagnetized. The Hayang Group, except for the remagnetized Shinyangdong Formation, yields the mean characteristic direction of $D/I=22.5^{\circ}/57.2^{\circ}$ (${\alpha}_{95}=4.6^{\circ}$, N=14 sites) and the pole position is $72.0^{\circ}N$, $206.4^{\circ}E$ ($dp/dm=4.9^{\circ}/6.7^{\circ}$). The Yucheon Group shows two polarities and the mean characteristic direction of $D/I=351.2^{\circ}/60.5^{\circ}$ (${\alpha}_{95}=11.2^{\circ}$, N= 19 sites) and the pole position is $81.3^{\circ}N$, $79.0^{\circ}E$ ($dp/dm=13.0^{\circ}/17.0^{\circ}$). The mean directions of both the Hayang and the Yucheon Groups are supported by the McElhinny's fold test at the 99% confidence level and that of the Yucheon Group by a reversal test at the 95% confidence level. A magnetostratigraphic correlation between polarities of the study formations and the Geomagnetic Time Scale indicates that the Hayang Group can be correlated to the Cretaceous Long Normal Superchron (CLNS), and the Yucheon Group to the boundary between the CLNS and the Polarity Chron 33R or later boundaries between normal and reverse polarities. Comparison of the paleopoles from this study with those from the surrounding areas both within the Gyeongsang basin and in the northeastern Asia indicates that the study area was not undergone significant tectonic rotations with respect to the other parts of the Gyeongsang basin and that the Korean Peninsula was the part of the single terrane of the northeastern Asia at least since the CLNS. The Yucheon Group can be divided into four sub-groups based on the paleomagnetic data, suggesting that there were at least four times of volcanic activities in the study area.