농약 Chlorothalonil 과 Command 의 수용성 및 증기압

김균,김영배,김용화,노정구,Kim, Kyun,Kim, Yong-Bae,Kim, Yong-Hwa,Roh, Jung-Koo 한국환경농학회 1987 한국환경농학회지 Vol.6 No.2

Chlorothalonil 과 Command 의 수용성과 증기압을 미국 EPA 와 OECD의 방법에 준하여 측정하였다. 두 화합물의 수용성은 문헌치와 잘 일치하였다. 그러나 chlorothalonil의 증기압은 handbook 에 나타난 수치보다 5,000배 낮았고($25^{\circ}C$에서 $2{\times}10torr$)의 Command 증기압은 100배 이상 낮음($45^{\circ}C$에서 $1{\times}10\;torr$ 이하)을 발견하였다. 따라서 handbook 류의 문헌의 물리화학적 특성치를 이용하여 환경동태를 예측하는 데는 주의를 요한다. 이상의 실험을 통하여 실험상의 난점들을 파악할 수 있었고 미국내에서 Command 가 주위작물에 준 표백현상은 증기확산에 의한 것이라기 보다는 살포시 유실에 의한 현상일 가능성이 높을 것으로 추론하였다. 이러한 접근방법은 새로운 화학물질의 개발단계에서 환경동태의 예측, 기존 화학물질의 오염가능성 예측, 오염저감 대책의 제시에 기초가 될 것으로 사료된다. The water solubilities and vapor pressures of chlorothalonil and Command were measured following the guidelines of the U.S. EPA and OECD. Water solubility of the two compounds is consistent with respective values in the literature. However, the vapor pressures of Chlorothalonil and Command were 5,000 times$(2{\times}10^{-7}\;torr\;at\;25^{\circ}C)$ and 100 times$(<1{\times}10^{-6}\;torr\;at\;45^{\circ}C)$ lower than the literature values, respectively. Courteous use of the vapor pressure values in the handbooks is suggested. With this study, experimental difficulties involved were recognized. Based on the low vapor pressure of Command, the cause of the accidental bleach of non-target plants in the United States might not be attributed to the volatilization of Command, but to the drift during the application of the herbicide. These approaches will be utilized to predict the environmental fate of new chemicals under development, to screen the potential environmental pollutants among chemicals already in use, and to assess measures to minimize the hazards.

Formaldehyde 가스 흡입에 의한 마우스의 급성독성 및 소핵 유발성에 관한 연구

김충용,김균,심점순,김용화,노정구,Kim, Choong-Yong,Kim, Kyun,Shim, Jeom-Soon,Kim, Yong-Hwa,Roh, Jung-Koo 한국독성학회 1991 Toxicological Research Vol.7 No.1

The acute and genetic effect of formaldehyde on mice through inhalation route was studied. The Riley's chamber with one stack of cage was used for the exposure and the micronucleus test was performed under unprecedently maximum exposure concentration. LC50's of formaldehyde in mice by whole body exposure for 4 hours were 105.5 ppm with 95% confidence interval of 72.6 ppm and 143.2 ppm for male, and 159.2 ppm with 95% confidence interval of 116.5 ppm and 272.7 ppm for female. Cinicial symptoms by acute exposure were salivation, lacrimation, and abnormal respiration.

Detection of Aflatoxins in Soybean Food by HPLC

Youngkook Kim(김영국),Jungkoo Roh(노정구) 한국식품과학회 1985 한국식품과학회지 Vol.17 No.4

수입옥수수 중 지아라레논의 검색

김영국,노정구 ( Y . K . Kim,J . K . Roh ) 한국축산학회 1986 한국축산학회지 Vol.28 No.2

Twenty corn samples from feed companies and farms were analyzed for zearlenone. Zearalenone was determined by using a high pressure liquid chromatography (HPLC) with Micropak MCH-10 column and methanol-water system and with an ultraviolet detector set at 274 ㎚. Zearalenone was detected in 8 samples at the level of 0.2-3 ㎎/Kg. And 2 samples contained more than 1 ㎎/Kg.

Detection of Aflatoxins in Soybean Food by HPLC

김영국,노정구,Kim, Young-Kook,Roh, Jung-Koo Korean Society of Food Science and Technology 1985 한국식품과학회지 Vol.17 No.4

고속액체 크로마토 그라피에 CN컬럼과 분리용매로 핵산-테트라 하이드로퓨란-아이소프로필알콜-물을 사용하고 형광 검출기의 검출관에 리클로솔브로 채워 아플라톡신 $B_1$, $B_2$, $G_1$, $G_2$를 분리 정량하었다. 이 조건에서 아플라톡신 $B_1$의 최저 검출한계는 0.2ng이었다. 대량생산 대두발효 식품과 메주에서 아플라톡신함량을 조시하였고, 대두 발효식품에서 사용되는 균주의 아폴라톡신 생성능을 알아보았다. TLC에서는 아플라톡신 유사물질이 검출되었지만 HPLC에서 아플라톡신이 아닌 것으로 나타났다. 시험한 균주의 아폴라톡신의 생성능은 없었다. Aflatoxin $B_1$, $B_2$, $G_1$, and $G_2$were quantitatively detected by the high pressure liquid chromatography on a Micropak-CN column, with Hexane-THF-IPA-water, using a Lichrosorbpacked flowceil in the fluorometric detector. Under those conditions, the minimum detectable amount of aflatoxin $B_1$ was 0.2 ng. HPLC was used in determining amount of aflatoxins in the commercially manufactured soybean food and home-made Meju. Aflatoxin producing abilities of strains used in the industrially fermented soybean food were also studied with the HPLC technique. Although aflatoxin-like substances were detected in a few samples on TLC, they were not identified with the HPLC retention times of standard aflatoxins. The commercial fungal strains used in Korea had no aflatoxin producing abilities.

Ktc : SD 랫드의 생식독성학적 기초자료연구

김성훈(Kim Sung-Hoon),정문구(Chung Moon-Koo),한상섭(Han Sang-Seop),노정구(Roh Jung-Koo) 한국실험동물학회 1992 Laboratory Animal Research Vol.8 No.1

Rat에 있어서 실험동물 사료의 멸균방법에 따른 사양실험에 관하여

김성훈(Kim Sung-Hoon),박종일(Park Jong-Il),한상섭(Han Sang-Seop),노정구(Roh Jung-Koo) 한국실험동물학회 1990 Laboratory Animal Research Vol.6 No.1

Beagle dog에 있어서 rifapentine의 독성동태연구

신호철,이혜숙,차신우,한상섭,노정구,김진석,이원창,Shin, Ho-chul,Lee, Hye-suk,Cha, Shin-woo,Han, Sang-seop,Roh, Jung-ku,Kim, Jin-suk,Lee, Won-chang 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.4

The toxicokinetics of rifapentine was studied after an oral administration to beagle dogs. High-performance liquid chromatography(HPLC) using column-switching technique was performed to determine the serum concentrations of rifapentine. The pharmacokinetic profiles of rifapentine were analysed using one-compartment open model. Following a single oral administration of 10mg/kg, pharmacokinetic parameters were determined as follows: maximum serum concentration($C_{max}$), $28.90{\mu}g/ml$; maximum concentration time($T_{max}$), 3.7hr; elimination half-life($t_{1/2}$, 4.7hr; area under the curve(AUC), $339.0{\mu}g{\cdot}hr/ml$; volume of disiribution/bioavailability (Vd/F), 0.21 l/kg; lag time, 24min; absorption rate constant($k_a$), $0.445hr^{-1}$; elimination rate constant($k_{el}$), $0.148hr^{-1}$. After 6 month multiple oral doses of 10mg/kg/day, parameters were as follows: $C_{max}$, $34.40{\mu}g/ml$; $T_{max}$, 2.6hr; $t_{1/2}$, 6.7hr; AUC, $391.3{\mu}g{\cdot}hr/ml$; Vd/F, 0.291/kg; $k_a$, $0.976hr^{-1}$; $k_{el}$, $0.104hr^{-1}$. The consistant kinetic parameters after a single and multiple oral administration show that there was no accumulation of rifapentine after 6 month oral administration. We also simulated the concentration of rifapentine after oral multiple administration of 10 and 50mg/kg/ day, based on the parameters obtained form the single administration. The measured serum concentrations of rifapentine were well fitted to the simulated results. The simulated results show that rifapentine readily reaches to steady-state after about 3 doses and the steady-state serum concentrations($C_{ss}$) are fluctuated in between $2.2{\sim}25.2{\mu}g/ml$, and $10.6{\sim}125.2{\mu}g/ml$ at the doses of 10 and 50mg/kg/day, respectively.

The effect of captafol on the hematological value, erythrocyte membrane and plasma biochemical value in vitro

김성훈,황화선,차신우,한상섭,노정구,Kim, Sung-hoon,Hwang, Hwa-seon,Cha, Shin-woo,Han, Sang-seop,Roh, Jung-koo The Korean Society of Veterinary Science 1993 大韓獸醫學會誌 Vol.33 No.3

Captafol은 phthalimide계통의 항진균제로써 difolatan이란 상품명으로 개발되어 과일, 채소의 탄저병 및 원목 등의 곰팡이 구제에 널리 사용되고 있는 것으로 국내에서도 두번째로 많이 생산, 사용되는 품목이나 독성실험에 관한 보고는 거의 없는 상태이다. 이에 본 연구에서는 captafol 자체에 대한 독성기전의 일부를 확인하고자 동물체내에서 일어나는 여러가지 대사작용이 배제된 시험관내에서 실험을 수행하였다. 랫트 혈액을 후대정맥으로 부터 채혈하여 4개의 시험관에 $2.5m{\ell}$씩 분주한 후 captafol 농도가 $0.1{\times}10^{-4}M$, $1{\times}10^{-3}M$, $1{\times}10^{-2}M$이 되도록 한 ethanol용액 $25{\mu}{\ell}$씩 넣고 $CO_2$ incubator에서 2시간 동안 부드럽게 혼합하여 혈액학적 성상 및 적혈구 취약성 시험을 수행하였다. 또한 원심분리하여 얻어진 혈장에서 생화학적 성상 등을 조사한 결과 다음과 같은 성적을 얻었다. 1. 적혈구수 및 hematocrit치는 captafol 투여량에 비례적으로 유의한(p<0.05) 감소를 나타내었다. 2. 적혈구막 취약성 실험에서 $1{\times}10^{-3}M$ 및 $1{\times}10^{-2}M$ 농도에서 대조군에 비하여 유의한(p<0.01) 증가를 나타내었다. 3. 혈장내 potassium이온의 농도는 captafol의 투여량에 비례적으로 증가하였다. 4. Total bilirubin 및 creatine kinase가 대조군에 비하여 각각 증가 및 감소하였다. The effect of captafol on the hematological value, erythrocyte membrane and plasma biochemical value was investigated using blood of SPF Sprague-Dawley male rats in vitro. For the anticoagulations, we used 0.5mg of heparin per $10m{\ell}$ of blood from vena cava. Three con-centrations($0.1{\times}10^{-4}M$, $1{\times}10^{-3}M$ and $1{\times}10^{-2}M$) captafol in ethanol were added to the each $2.5m{\ell}$ blood so that the linal concentration of ethanol was 1%. The blood contained with each concentration of captafol was incubated at $37^{\circ}C$ C for 2 hours under 5% $CO_2$ gas The whole blood and plasma were examined for hematological vaJues, erythrocyte membrane damage and biochemical values, respectively. The results obtained were summarized as follows ; 1. The number of RBC in $1{\times}10^{-2}M$ group and the concentration of MCHC in $1{\times}10^{-3}M$ group were significantly (p<0.05) decreased and increased from that of control values, respectively. The percentage of Hct was significantly (p<0.05) decreased with dose-response. 2. Erythrocyte fragility rate of $1{\times}10^{-3}M$ and $1{\times}10^{-2}M$ group were significantly (p<0.01) increased from that control with dose response. 3. Potassium ion level of $1{\times}10^{-2}M$ was significantly (p<0.05) increased from that of control. 4. The concentration of total bilirubin in the $1{\times}10^{-3}M$ and $1{\times}10^{-2}M$ groups were significantly increased from that of control. The enzyme level of creatine kinase in $1{\times}10^{-2}M$ group was significanlty (p<0.05) decreased from control value.

무균랫트의 생산에 관한 연구

김형진(Hyoung-chin Kim),이영순(Yong-soon Lee),노정구(Jung-koo Roh) 한국실험동물학회 1985 Laboratory Animal Research Vol.1 No.1