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약물 및 유전자 전달체로 응용하기 위한 Poly(L-Lysine)이 결합된 O-Carboxymethyl Chitosan PEG의 제조와 특성
남정표 ( Joung Pyo Nam ),김영민 ( Young Min Kim ),박진수 ( Jin Su Park ),이응재 ( Eung Jae Lee ),최창용 ( Chang Yong Choi ),장미경 ( Mi Kyeong Jang ),나재운 ( Jae Woon Nah ) 한국공업화학회 2010 공업화학 Vol.21 No.6
키토산의 응용성을 높이기 위해 제조된 O-carboxymethyl water soluble chitosan (OCMCh)의 구조에 인체 내 순환시간을 증가시키기 위하여 PEG를 도입하였으며, 약물 및 유전자 전달제로 응용하기 위하여 PEG가 결합된 OCMCh-PEG를 Poly(L-Lysine) (PLL)과 이온복합체를 형성함으로써 OCMCh-PEG-PLL를 제조하였다. 제조된 OCMCh-PEG-PLL의 물리 화학적특성은 적외선 분광광도계와 핵자기공명장치를 이용하여 분석하였으며, 성공적으로 PLL이 결합되었음을 확인 하였다. 또한 동적광산란장치와 투과전자현미경을 통하여 PLL의 양을 고정하였을 때, PEG의 양이 중가함에 따라 입 자의 크기가 감소하는 것을 볼 수 있었으며, 구형의 업자형태를 가지는 것을 확인할 수 있었다. 이상의 결과는 OCMCh-PEG-PLL이 약물 및 유전자 전달체 등과 같은 생체재료로의 응용 가능성을 가지는 것을 볼 수 있다. O-carboxymethyl water-soluble chitosan (OCMCh) prepared for enhance the application of chitosan was modified with mthoxy polyethyleneglycol (mPEG) by ion-complex for long circulation in the blood. OCMCh-PEG-PLLs was prepared by forming ion-complex with OCMCh-PEG and Poly(L-Lysine) (PLL) for drug and gene delivery system. The physicochemcal characterisitcs of OCMCh-PEG-PLLs were investigated by FT-IR, (1)H-NMR. These results showed that CMCh-PEG-PLLs were successfully syntehsized by ion-complex. Particle size distribution and zeta potential of the OCMCh-PEG-PLLs were determined using dynamic light scattering technique. Transmission electron microscopy (TEM) was also used to observe the morphology of the OCMCh-PEG-PLLs. OCMCh-PEG-PLLs have spherical shapes with particle size 290~390 nm. OCMCh-PEC-PLLs were showed when the feeding amount of mPEG ratio was increased, particle size and zeta potential were decreased. Based on these results, it is possible to introduction of the OCMCh-PEG-PLLs into various biomedical fields such as drug and gene delivery system.
이온 complex 형성에 의한 Cisplatin이 봉입된 Carboxymethyl Chitosan 나노입자의 제조
남정표 ( Joung Pyo Nam ),김동곤 ( Dong Gon Kim ),정영일 ( Young Il Jeong ),김명렬 ( Myung Yul Kim ),장미경 ( Mi Kyeong Jang ),나재운 ( Jae Woon Nah ) 한국키틴키토산학회 2008 한국키틴키토산학회지 Vol.13 No.1
Carboxymethyl chitosan(CMCh) was prepared by introduction of caroxymethyl group to C6-OH (O-carboxymethyl chitosan, OCMCh) or C2-NH2 (N-carboxymethylc chitosan, NCMCh) position of low molecular weight water soluble chitosan (LMWSC). Composition of CMCh was confirmed by proton-nuclear magnetic resonance spectroscopy (1H NMR). Cisplatinincorporated nanoparticles were prepared by simple mixing of cisplatin and CMCh. Nanoparticles can be prepared by ion complex formation between cisplatin and CMCh. When the feeding amount of cisplatin was increased, drug contents was increased and loading efficiency was decreased. Furthermore, particle size became larger size and zeta potential was decreased by increasing the feeding amount of drug. At the observation of transmission electron microscopy (TEM), cisplatin-incorporated CMCh nanoparticles have spherical shapes with diameter of about 100 nm ~ 300 nm. At drug release study, cisplatin release rate was decreased by increased feeding amount of cisplatin. Nanoparticles from OCMCh showed faster cisplatin release properties than that of NCMCh. Cell cytotoxicity of cisplatin-incorporated CMCh nanoparticles were tested with human embryonic kidney cell, 293T. In the case of cisplatin itself, survived cells were gradually decreased by increasing the concentration of cisplatin from 0.01 ug/ml to 100 ug/ml. When nanoparticles were treated, however, higher than 80% of cells were survived at 10 ug/ml equivalent concentration of cisplatin and about 70% of cells were survived at 100 ug/ml. These results showed that cisplatinincorporated CMCh nanoparticles can be considered as a predominent cisplatin delivery carriers.
N,O와 N-O Carboxymethyl Chitosan의 NMR 기능을 이용한 분광학적 특성분석
남정표 ( Joung Pyo Nam ),허선행 ( Sun Heang Heo ),정현 ( Hyun Jung ),김동곤 ( Dong Gon Kim ),정영일 ( Young Il Jeong ),장미경 ( Mi Kyeong Jang ),나재운 ( Jae Woon Nah ) 한국공업화학회 2009 응용화학 Vol.13 No.1
Carboxymethyl chitosan (CMCh) was prepared by chemical reaction with mono-chloroacetic acid and glyoxylic acid to low molecular weight water soluble chitosan (LMWSC) of C6-OH (O-Carboxymethyl chitosan), C2-NH2 (N-Carboxymethylc chitosan) and both C6-OH and C2-NH2 (N,O-Carboxymethyl chitosan) group to apply as an ionic-drug carrier. The structure were characterized by 1H-NMR, 13C-NMR, DEPT 135 and HMQC (Heteronuclear Multiple Quantum Correlation). Degree of substitution (DS) and degree of deacetylation (DDA) of CMCh was determined by 1H-NMR.