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백합(Meretrix meretrix) 식해에서 분리한 Pediococcus pentosaceus SH-10에 의한 병원성 세균의 억제 기작
신동민 ( Dong Min Shin ),김희대 ( Hee Dai Kim ),구재근 ( Jae Geun Koo ),박권삼 ( Kwon Sam Park ) 한국수산과학회 2012 한국수산과학회지 Vol.45 No.6
In this study, we investigated the mechanism of inhibition of pathogenic bacteria by Pediococcus pentosaceus strain SH-10 isolated from hard Clam Meretrix meretrix sikhae. When P. pentosaceus SH-10 was co-cultured in MRS broth with pathogenic bacteria, including Bacillus cereus, Listeria monocytogenes, Salmonella choleraesuis and Staphyloccus aureus, no viable pathogenic cells were detected after 18 h of incubation. However, pediocin or a pediocin-like bacteriocin was not detected in cultures of P. pentosaceus SH-10 by the agar diffusion method. Organic acids were produced in MRS broth in proportion to the incubation time of P. pentosaceus SH-10. These results indicate that P. pentosaceus SH-10 inhibited the growth of pathogenic bacteria by lowering the pH of the growth medium through the production of organic acids, including sodium lactate, sodium acetate, and sodium citrate.
곰소만 해역 해수에서 분리한 장염비브리오(Vibrio parahaemolyticus)의 항균제 내성 및 최소발육억제농도의 규명
김태옥 ( Tae-ok Kim ),엄인선 ( In-seon Um ),김희대 ( Hee-dai Kim ),박권삼 ( Kwon-sam Park ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.5
Seventy-nine Vibrio parahaemolyticus isolates from surface seawater from Gomso Bay, west coast of Korea, were analyzed for the presence of virulence genes and their susceptibility to 30 different antimicrobials. All 79 isolates were examined for the presence of two virulence genes (tdh or trh) using polymerase chain reaction (PCR); however, no isolates possessed either the tdh or trh gene. According to a disk diffusion susceptibility test, all of the strains studied were resistant to oxacillin, penicillin, and vancomycin, followed by ticarcillin (97.5%), ampicillin (96.2%), clindamycin (86.1%), erythromycin (10.1%), streptomycin (7.6%), cefoxitin (6.3%), amikacin (2.5%), and cephalothin (2.5%). However, all of the strains were susceptible to 19 other antimicrobials including cefepime, cefotaxime, chloramphenicol, gentamycin, nalidixic acid, sulfamethoxazole/trimethoprim, and trimethoprim. All 79 isolates (100%) were resistant to four or more classes of antimicrobials, and two strains exhibited resistance to eight antimicrobial agents. The average minimum inhibitory concentrations (MICs) for V. parahaemolyticus for ampicillin, penicillin, ticarcillin, and vacomycin were 946.5, 1,305.9, 1,032.3, and 45.0 μg/mL, respectively.
완도해역 해수에서 분리한 장염비브리오(Vibrio paragaemolyticus)의 항균제 내성 및 병원성 유전자의 특징
김태옥 ( Tae Ok Kim ),엄인선 ( In Seon Ewn ),조상만 ( Sang Man Jo ),김희대 ( Hee Dai Kim ),박권삼 ( Kwon Sam Park ) 한국수산과학회 2014 한국수산과학회지 Vol.47 No.3
Sixty-seven Vibrio parahaemolyticus isolates from surface seawater from the Wando area, on the southern coast of Korea, were analyzed for their susceptibility to 15 different antimicrobials and the presence of virulence genes. According to the disk diffusion susceptibility test, all of the strains studied were resistant to ampicillin and oxacil-lin, while decreasing percentages were resistant to vancomycin (64.2%), streptomycin (56.7%), amikacin (31.3%), kanamycin (22.3%), cephalothin (20.9%), erythromycin (10.4%), ciprofloxacin (4.5%), and tetracycline (3.0%). All of the strains were susceptible to five antimicrobials: chloramphenicol, gentamycin, nalidixic acid, sulfamethoxazolel/ trimethoprim, and trimethoprim. Fifty-nine isolates (88.1%) were resistant to three or more classes of antimicrobial and defined as multidrug resistant, and two strains were resistant to seven antimicrobial agents. The minimum inhibi-tory concentration (MIC) of the 67 V. parahaemolyticus isolates to ampicillin and oxacillin ranged from 512-2,048 and 64-512 ㎍/mL, respectively. All 67 isolates were also examined for the presence of the tdh and trh virulence genes using the polymerase chain reaction (PCR). However, no isolates possessed either tdh or trh. The VPA0477 (β-Iactamase) gene, present in all of the tested strains, was validated as a new specific marker gene in PCR assays for the accurate detection and identification of V. parahaemolyticus.
가민경(Ka, Min-Kyoung),김미혜(Kim, Mi-Hye),김봉현(Kim, Bong-Hyun),김희대(Kim, Hee-Dai),조동욱(Cho, Dong-Uk) 한국산학기술학회 2014 한국산학기술학회논문지 Vol.15 No.5
현대인들은 매운 음식을 선호하며 매운 음식을 섭취함으로써 스트레스 해소를 위해 매운맛을 찾는 사람들이 증가하 고 있다.그러나,매운 음식을 먹었을 경우,사람마다 차이는 있지만 얼굴색이 빨갛게 변하는 것을 시각적으로 확인할 수 있다.따라서 본 논문에서는 매운 음식을 섭취했을 경우,사상체질에 따라 얼굴 영역별 색상 변화를 측정하고 이를 비교, 분석하는 실험을 수행하였다.이를 위해 체질감별 설문 검사지를 통해 사상체질별로 피실험자 집단을 구성하고 대표적인 매운 음식인 청양고추 1개씩 섭취하기 전과 후의 얼굴 영상을 기반으로 Lab색 체계를 적용한 영상신호분석 실험을 통해 사상체질별 매운 음식과 얼굴 색상과의 상관성 분석 연구를 수행하였다. These days, there is increasing those who like spicy food, people release stress by eating spicy food. But, when you eat spicy food, there is a difference but, we visually can be found red facial color to change. In this paper, when you eat spicy food, we carried out experiment which comparison and analysis color change of facial area depending on Sasang constitutional type. To this end, we organized test subject group by Sasang constitutional type according to survey result for Sasang constitutional type. And then we carried out interrelationship analysis between spicy food and facial color depending on facial color to apply Lab color system based on facial image which is before and after eat a hot spicy pepper.
백합(Meretrix meretrix )식해에서 분리한 Pediococcus pentosaceus SH-10의 생균제적 특성
송현정 ( Hyun Jung Song ),김강진 ( Kang Jin Kim ),김희대 ( Hee Dai Kim ),유정희 ( Jung Hee Yoo ),구재근 ( Jae Geun Koo ),박권삼 ( Kwon Sam Park ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.6
This study examined the suitability of characteristics of potential strains of probiotic bacteria. Among 25 lactic acid bacteria isolated from Korean traditional fermented food, the Hard Clam Meretrix meretrix Shikhae, the SH-10 strain, which exhibited superior resistance to low pH and bile salts, was selected as a potential probiotic bacteria. By examining carbohydrate utilization, morphological properties, and the 16S rRNA gene sequence, the SH-10 strain was identified as Pediococcus pentosaceus (hereafter, P. pentosaceus SH-10). P. pentosaceus SH-10 was resistant to amikacin, cefotetan, ciprofloxacin, gentamicin, kanamycin, nalidixic acid, streptomycin, and vancomycin. Tests of antimicrobial activities against pathogens such as Bacillus cereus , Listeria monocytogenes , Salmonella choleraesuis , and Staphylococcus aureus , indicated that P. pentosaceus SH-10 inhibited the growth of pathogenic bacteria. These results suggest that P. pentosaceus SH-10 can be developed as a probiotic bacteria.
시판 까나리(Ammodytes personatus) 액젓에서 분리한 tryptamine 생성균의 특성
엄인선 ( In-seon Um ),김태옥 ( Tae-ok Kim ),김희대 ( Hee-dai Kim ),박권삼 ( Kwon-sam Park ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.6
We isolated seven tryptamine-producing bacteria from commercial salted and fermented sand lance (Ammodytes personatus) sauces using an L-tryptophan decarboxylating medium. These tryptamine-producing bacteria, identified using an API kit and 16S rRNA analysis, included Lysinibacillus xylanilyticus (one strain), Lysinibacillus fusiformis (four strains), and Staphylococcus epidermidis (two strains). Lysinibacillus spp. produced the highest levels of tryptamine in culture broth containing 0.5% L-tryptophan, compared with 1.0% and 2.0% preparations. After 72 h of incubation, Staphylococcus epidermidis produced the highest levels of tryptamine (60.50 μg/mL and 664.86 μg/ mL) in culture broth containing 2.0% L-tryptophan. While Lysinibacillus spp. comprised the dominant tryptamine-producing bacteria in sand lance sauces, Staphylococcus epidermidis also showed high tryptamine-producing active-ity. This is the first report on the isolation and identification of tryptamine-producing bacteria in sand lance sauces.
장염비브리오가 보유하는 ß-Lactamase (VPA0477)의 유전학적 특성
이남형 ( Nam Hyung Lee ),송현정 ( Hyun Jung Song ),박창수 ( Chang Soo Park ),김희대 ( Hee Dai Kim ),박권삼 ( Kwon Sam Park ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.6
Using 108 strains of Vibrio parahaemolyticus isolated from seawater, we investigated ampicillin-resistance profiles and the genetic characterization of ß-lactamase (VPA0477). All of the strains studied, except one strain, were resistant to ampicillin. However, the strain that was susceptible to ampicillin had the same ß-lactamase gene as the ampicillinresistant strains. We compared ß-lactamase promoter region sequences among five strains, including both ampicillinresistant and -susceptible strains. In the susceptible strain, a nucleotide at position -19 in the methionine initiation codon for ß-lactamase was not present in the ampicillin-resistant strains. The genes in the region containing the gene VPA0477 were present in all of the tested strains, and LA-PCR analysis showed that the distance between VPA0474 and VPA0479 in all of the V. parahaemolyticus samples was precisely 5.7 kb. In V. parahaemolyticus ß-lactamase, four important structural features that are conserved in Class A ß-lactamases were present in the deduced amino acid sequences. Taken together, our study demonstrates that V. parahaemolyticus ß-lactamase is included in the Class A ßlactamase group, and some nucleotides within the promoter region are of particular importance for ß-lactamase activity.