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白鼠舌背粘膜의 蛋白合成와 移動에 關한 自記放射法的 硏究
金孝喆 최신의학사 1974 最新醫學 Vol.17 No.6
Forty-five male mice weighing approximately 20g were used in this study. They received a single intraperitoneal injection of 3pc/g of body weight of phenylalanine -3H (specific activity 3 c/mM) obtained from New England Nuclear Corporation. Mice receiving phenylalanine 'H were sacrificed at 1 hour, 4 hours, 1,4.5.7,9,11 and 14 days after injection. Dorsal tongue of all animals were immediately removed and fixed in 2% paraformaldehyde in cacodylate buffer, sectioned at 4p and stained with toluidine blue O. The slides are dipped into Kodak NTB3 liquid emulsion and exposured for two weeks. Following the exposure, the slides are developed for 1 minutes at 18ºC in a developer and fixed in kodak rapid fixer. The present investigation was undertaken primarily to determine more precisely the turnover time of dorsal lingual mucosa by autoradiographic analysis. The results were as follows. 1. Most cells in the stratum basale of the dorsal lingual. epithelium were radioactived at one day after injection. 2. Phenylalanine labeled cells were reached the lower half portion of the stratum spinosum in 4 days after injection. 3. Seven days after injection radioactive cells had moved up the upper half of stratum s0losum. 4. Tritiated phenylalanine was immediately incorporated into all differentiating epithelial layers, entering the stratum corneum in 9 days after injection, and all labelling was gone by day 14. 5. These and similar observations may be of value in future studies of pathological processes involving
김효철 啓明專門大學 産業開發硏究所 1999 啓明硏究論叢 Vol.17 No.2
본 연구에서는 현재 인터넷 상에서 클라이언트/서버 간의 상호대화형 접속을 위한 백본으로 널리 사용되고 있는 HTTP/CGI 프로토콜과 머지 않은 장래에 이것을 대체 할 것으로 예상되는 CORBA/IIOP 기반 기술을 검토하고 그 성능을 비교하였다. 또한 CORBA/IIOP의 기존 제품들이 고속 네트워크에서 적용되었을 경우 발생할 수 있는 오버헤드의 종류와 오버헤드를 최소화하기 위한 전략을 제시하였다. The study examines and compares performance of HTTP/CGI protocol and CORBA/IIOP technologies. HTTP/CGI is currently used as backbone of interactive connection in Internet client/server system and CORBA/IIOP will replace HTTP/CGI as backbone soon. Therefore, reveals kinds of CORBA/IIOP overheads and minimizing strategies of overheads.