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김효준,Lee Jin-Haeng,Lee Ki Baek,Shin Ji-Woong,Kwon Mee-ae,Lee Soojin,Jeong Eui Man,Cho Sung-Yup,Kim In-Gyu 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-
Glutathione S-transferase (GST) from Schistosoma japonicum has been widely used as a tag for affinity purification and pulldown of fusion proteins to detect protein–protein interactions. However, the reliability of this technique is undermined by the formation of GST-fused protein aggregates after incubation with cell lysates. It remains unknown why this aggregation occurs. Here, we demonstrate that the GST tag is a substrate of transglutaminase 2 (TG2), which is a calcium-dependent enzyme that polyaminates or crosslinks substrate proteins. Mutation analysis identified four glutamine residues in the GST tag as polyamination sites. TG2-mediated modification of the GST tag caused aggregate formation but did not affect its glutathione binding affinity. When incubated with cell lysates, GST tag aggregation was dependent on cellular TG2 expression levels. A GST mutant in which four glutamine residues were replaced with asparagine (GST 4QN ) exhibited a glutathione binding affinity similar to that of wild-type GST and could be purified by glutathione affinity chromatography. Moreover, the use of GST 4QN as a tag reduced fused p53 aggregation and enhanced the induction of p21 transcription and apoptosis in cells treated with 5-fluorouracil (5-FU). These results indicated that TG2 interferes with the protein–protein interactions of GST-fused proteins by crosslinking the GST tag; therefore, a GST 4QN tag could improve the reproducibility and reliability of GST pulldown experiments.
개방형 탐구 및 모의 실험수업 경험이 예비물리교사의 인식에 미치는 영향 탐색
김효준,하상우 한국물리학회 2023 새물리 Vol.73 No.4
Experiments are fundamental aspect of scientific inquiry and its role in science education is crucial. However, many experimental classes in schools tend to focus on confirmation experiments that limit students’ ability to explore and inquire. This study aims to help preservice teachers in understanding the value of inquiry-based experimental classes by inviting them to participate in an open-ended inquiry. Fifteen preservice teachers who had minimal prior experience in conducting an open inquiry and teaching formal classes participated in this study. Through this study, they were able to identify the benefits of an open-ended experiment, noting that it was enjoyable and beneficial in fostering a deeper understanding of concepts. Moreover, they observed that open-ended inquiry is similar to what scientists do and thus must be experienced by students. They thought that laboratory instruction was crucial for effective teaching in schools. Nevertheless, they acknowledged that teaching experimentation requires substantial preparation and an in-depth understanding of the experiment. 실험은 과학의 중요한 활동 중 하나이므로 과학교육에서도 그 역할이 필수적이다. 하지만 학교 현장에서의 실험수업은 탐구 방법을 일방적으로 제시하는 확인 실험의 형태로 많이 진행되어 오히려 부정적인 효과가 나타난다. 본 연구는 예비교사들이 개방형 탐구 및 모의실험수업 진행 경험을 통해 탐구와 실험수업의 가치를 깨닫게 하려는 목적으로 수행되었다. 본 연구의 참여자는 사범대학 물리교육학과에서 중등물리실험지도 교과를 수강하는 15명의 예비교사였으며, 이들 대부분은 개방형 탐구 및 정식 수업 진행 경험이 거의 없었다. 연구를 위해 학기말 보고서, 수업 분석 기록지, 면담 자료가 활용되었으며, 모든 자료는 질적으로 분석되었다. 연구 결과 예비교사들은 개방형 탐구를 통해 개방형 실험의 가치를 깨닫고, 개방형 탐구가 즐거울 뿐만 아니라 개념 이해에도 도움이 된다고 표현했다. 특히 개방형 탐구는 과학자가 하는 일에 가까우므로, 학생들에게도 이를 경험하게 해줘야 한다고 생각했다. 또한 예비교사들은 모의 실험수업을 통해 실험수업이 재미있으며, 학교 현장에서의 수업에서도 필수적이라는 것을 깨달았다. 하지만 실험수업은 준비에 많은 노력이 필요하며, 실험 내용에 대한 교사의 철저한 이해가 필요하다고 생각했다.
김효준,손민희,안성훈,이선영 한국물리학회 2020 Current Applied Physics Vol.20 No.6
Tungsten oxide is an electrochromic material, from which thin films are conventionally fabricated via heat treatment. Here, we develop a flash light sintering method to reduce sintering time; sintering delivers instantaneous photon energy created via the photothermal effect. The electrochromic efficiency of device with a flash light-sintered electrode was 88.50 cm2/C, about 1.46-fold greater than that of the device with a heatsintered electrode. The diffusion coefficients of the former device were 1.35- and 1.46-fold greater than those of the latter device during bleaching and coloring, respectively. Therefore, a new way of sintering electrode was studied to show that the device with flash light–sintered electrode demonstrate better electrochromic performance than the device with heat-sintered one by enabling amorphous phase formation.
슬라이딩모드 제어기의 폐루프계 특성을 고려한 주파수성형 제어기법 연구
김효준 三陟大學校 2004 論文集 Vol.37 No.-
This paper presents the design of an active vibration suppression controller for a suspension system. Firstly, sliding mode controller is constructed considering the quarter car model of a vehicle Secondly. the closed-loop characteristics of sliding mode control system is investigated. An active control system is designed based on frequency-shaped sliding mode control scheme regarding the high frequency mode with respect to undesirable oscilation of tue mode Finally, the performance of the active control system is evaluated by simulation under uneven road disturbance condition.
기니픽 내이의 Deiters 세포에서 측정된 외향 정류성 칼륨이온전류
김효준,안중호,김원태,임채헌,정종우,이광선 대한이비인후과학회 2002 대한이비인후과학회지 두경부외과학 Vol.45 No.6
Background and Objectives:Otosclerosis is known to be rare in orientals, but there have been a few reports of the cli-nically suspicious otosclerosis cases in Korea. Confirmation of otosclerosis, in theory, should be done by histopathology. research, we had surgical specimens available for a review of otosclerosis case. We aimed to confirm the pathologic findings of otosclerosis in Koreans, and review their clinical information. Subjects and Method:Among 126 otosclerosis patients who were operated in the Seoul National University Hospital from January 1981 to December 2001, three cases were selected for pathologic evaluation. We used the H & E method for staining. Results:in all three cases. Two typical phases (early spongiotic and late sclerotic of otosclerosis) could be observed in specimen. Conclusion:In the pathologic specimen from the patients showing progresive conductive hearing loss, we could confirm otosclerosis. Both early and late phase of otsclerotic foci coexisted in the same specimen. (Korean J Otolaryngol 2002; 45:557-60)
김효준,박혁성,양현석,박영필 한국소음진동공학회 1997 소음 진동 Vol.7 No.2
This paper presents an active suspension control algorithm to improve the suspension performance trade-offs between riding comfort and handling stability. In this paper, a hybrid control scheme is proposed, the idea of which is that sliding mode control is used for nonlinear hydraulic system and the skyhook control is applied to control the vehicle behavior. The parameter variations in hydraulic system are considered for the robust controller design. The performance of the proposed control method is evaluated by simulation and experiments based on a half car roll model which can reveal both heave and roll behavior.