http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Mercuric chloride 유발 신부전에서 산소유리기에 미치는 Sodium selenite의 영향
김형춘,주왕기,허인회,Kim, Hyoung-Chun,Jhoo, Wang-Kee,Huh, In-Hoi 대한약학회 1988 약학회지 Vol.32 No.4
Oxygen free radical have recently been found to mediate cell injury after ischemia in the kidney. The purpose of our study was to determine whether selenium had an effect on damge mediated by oxygen free radical in inorganic mercury induced renal failure, toxic model of renal failure. Toxic renal failure model was produced by subcutaneous injection of mercuric chloride (4mg/kg) once a day for 7 consecutive days. In additionally, coadministration of sodium selenite (1mg/kg) was performed by the same condition. As a consequence of this study, we were able to detect partially unequivocal role of selenium as below dipicted. The combination of sodium selenite showed that markedly inhibited production of superoxide radical in mercuric chloride alone. On the other hand, combined sodium selenite was unable to enhance against significantly lowered superoxide dismutase activity after mercuric chloride insult. However, simultaneous administration of sodium selenite was inclined to induce mitochondrial superoxide dismutase and catalase.
Doxorubicin유발 심장독성에 미치는 L-Carnitine의 효과
김형춘,송계용,전구석,허인회,Kim, Hyoung-Chun,Song, Ke-Yong,Jeon, Ku-Seok,Huh, In-Hoi 대한약학회 1988 약학회지 Vol.32 No.4
This study was undertaken to elucidate the role of L-carnitine on cardiotoxicity induced by doxorubicin in mice. We designed to investigate both lipoperoxidation and histopathology in experimental group. The results obtained from prior with concurrent treatment of L-carnitine showed as follows; 1) Combination of L-carnitine showed significant inhibition of lipoperoxide in liver than heart. 2) By means of electron microscopy, we obtained histological evidence that doxorubicin-induced cardiomyopathy, is prevented by L-carnitine.
김형춘(Hyoung Chun Kim),전완주(Wan Jhoo Chun),이현우(Hyun Woo Lee),권명상(Myung Sang Kwon),송계용(Kye Yong Song),주왕기(Wang Kee Jhoo) 대한약학회 1992 약학회지 Vol.36 No.2
This study investigated the hypothesis that carcinogen-induced elevation of oxyradical during the hepatocarcinogenesis in rat. The hepatic preneoplastic lesions in the Spraque-Dawley rats were induced by the carcinogen treatment such as diethylnitrosamine(DEN) and acetylaminofluorene(AAF) in combination with partial hepatectomy(PH). The liver sample was taken at 2,6,10 and 16 months after carcinogen treatments followed by PH. Carcinogen treatments initially increased the indices of oxidative damage(activities of xanthine oxidase and production rates of superoxide anion, microsomal hydrogen peroxide, hydroxyl radical ) in the liver compared to PH groups. However, cytosolic hydrogen peroxide did not change significantly throughout the full time period. Of hydrogen peroxide scavenger, the catalase was remained lower than PH groups, whereas the peroxidase was increased after carcinogen treatments. Morphologically, the immunohistochemical analysis with glutathione-S-transferase of a placenta form(GSTP) antibody was used to detect the induction of preneoplastic nodules. During the hepatocarcinogenesis, both production rate of hydroxyl radical and activity of glutathione-S-transferase(GST) markedly increased with the appearance of the preneoplastic nodule. These results indicated that the hydroxyl radical of reactive oxygen species seemed to have a major influence on the hepatocarcinogenesis and the effect of time after removal of the carcinogen also appeared to be highly critical in the hepatocarcinogenesis.
사염화탄소-유발지방간에 대한 L-글루탐산 일나트륨의 보호작용
김형춘(Hyoung Chun Kim),이왕섭(Wang Seop Lee),전완주(Wan Jhoo Chun),최용순(Yong Soon Choi),김수희(Soo Hee Kim),이현우(Hyun Woo Lee),주왕기(Wang Kee Jhoo) 대한약학회 1992 약학회지 Vol.36 No.1
To achieve better understanding of the effects of monosodium-L-glutamate(MSG) against CCl4 fatty liver in Wister male rats, 5% MSG solution was given as drinking water and CCl4 0.1ml/kg was injected subcutaneously twice a week for four weeks. It was showed that increased hepatic phospholipid and hepatic triacylglycerol levels by CCl4 challenge were significantly decreased by additional MSG, respectively. However, MSG had no apparent effect on the elevated hepatic cholesterol level in the presence of CCl4. Histrionically, additional MSG markedly inhibited fatty degeneration, spotty necrosis, inflammation and periportal vascular proliferation manifested by CCl4. respectively. Theses results indicated that effects of MSG against CCl4 induced-fatty liver appeared to be involved with partial restoration of altered hepatic lipid composition.
사염화탄소 및 에탄올에 의해 유도된 만성간 손상에 미치는 말로틸레이트의 항산화 작용
김형춘(Hyoung Chun Kim),허인회(In Hoi Hur) 대한약학회 1990 약학회지 Vol.34 No.4
To achieve a better understanding of antioxidant action manifested by malotilate, the dithiol malonates, we monitored the oxy radical-scavenging system against the chronic hepatic damage induced by CCl4 alone or in combination with ethanol. Malotilate was given orally at a dose of 100mg/kg/day and CCl4 1.5ml/kg was injected subcutaneously twice a week for 4 weeks. In each group receiving ethanol, drinking water was replaced by 20% aqueous solution or glucose, isocaloric amounts of ethanol, as a control of ethanol was diluted in its drinking water. Each rat was killed as a starved state at 18 hours after the period of the experiment, four weeks. The results were summarized as follows: 1)Malotilate inhibited the rate of generation of superoxide radicals, the accumulation of lipoperoxides, and promoted the synthesis of glutathione in the liver. 2)Malotilate stimulated the enhancement of activity of superoxide dismutase in hepatic mitochondria. 3)Malotilate had no effects on the hepatic H202 contents. 4)Malotilate showed the increase of catalase activity in the liver poisoned with CCl4, and also gave a tendency to increase it in the liver intoxicated with ethanol. Thus, our data suggested that the activation of hepatic antioxidant system in the presence of malotilate would play a role in protecting liver against the toxic effects of oxy radical and/or lipid peroxides under the hepatotoxic conditions induced by CCl4 with or without ethanol. However, the effects of malotilate against the ethanol-induced hepatotoxicity appear to be insignificant.
사염화탄소와 Monosodium-L-Glutamate 병용투여에 의한 간조직의 환원형글루타치온 함량 및 그의 관련효소활성의 변화
김형춘(Hyoung Chun Kim),이왕섭(Wang Seop Lee),전완주(Wan Jhoo Chun),김수희(Soo Hee Kim),주왕기(Wang Kee Jhoo) 大韓藥學會 1991 약학회지 Vol.35 No.5
To explore the effect of monosodium-L-glutamate(MSG) on CCl4-damaged liver in Wister male rat, 5% MSG solution as drink water were administered after S.C. injection of 0.1 mg/kg CCl4 twice a week for 4 weeks. After last administration of MSG, heptic glutathione(GSH) dependent system was assayed. It showed that MSG increased significantly hepatic glutathione(GSH) and glutathione peroxidase(GSHpx), but decreased glutathione-S-transferase(GST) activity in normal rats. MSG increased significantly the GSHpx. and GST activities in rats with CCl4-induced liver damage. These results indicate that decrease of GSH dependent systems in CCl4 liver injury might be partially elevated by coadministration of MSG.
발암원으로 유도된 흰쥐간의 태반형 Glutathione S-Transferase 양성 증식성결절의 병리조직학적 연구
최기영,김형춘,송계용,박상철 중앙대학교 의과대학 의과학연구소 1988 中央醫大誌 Vol.13 No.3
A hlstopathologic and immunohistochemical studies were carried out on the hyperplastic nodule induced by the Solt and Farber method to observed the morphologic and histochemical enzymatic changes on the hyperplastic hepatocytes with aid of anti-glutathione S-transferase of placenta form (GST-P) antibody. The results obtained were as follows; 1. The transformed hepatocytes could be detected easily by use of GSI-P antibody as early as two weeks later since administration of diethylnitrosoamine only and promoted by additional treatment of acetylaminofluorene and partial hepatectomy. 2. The transformed hepatocytes destined to forming hyperplastic nodule were individually scattered at first in multiple foci which is very difficult to find until 3 weeks after pnsthepatectorny in H & E stained section 3. Thc transformed hepatocytes consisting of hyperplatic nodule are enlarged clear cells which are proved to have markedly increased amount of glycogen, smooth and rough endoplasmic reticulum by electron microscopy. 4. Area of GST-P positive hepatorytes were progressively and significantly increased by posthepatectomy 3 weeks. Therfore, glutathione S-transferase is a good marker forearly detection of hyperplastic or preneoplastic nodule induced by diethylnitrosoamine with acetylaminofluorene and the clear hyperplastic hepatocytes was the result of marked increase of glycogen and endoplasmic reticulum.