기니픽과 마우스에서 CFC-101 ( 녹농균 백신 ) 의 항원성시험

선우연(Woo Yearn Sun),한형미(Hyung Mee Han),김현수(Hyun Su Kim),백남진(Nam Jin Baek),김달현(Dal Hyun Kim),이동억(Dong Eok Lee),정승태(Seung Tae Chung),김필선(Pil Sun Kim) 한국응용약물학회 1994 Biomolecules & Therapeutics(구 응용약물학회지) Vol.2 No.4

As a part of the safety evaluation of Pseudomonas vaccine(CFC-101), antigenicity tests were carried out in guinea pigs and mice. In active systemic anaphylaxis(ASA) test, guinea pigs showed no sign or only moderate sign(1/5) when sensitized and challenged with up to 200 ㎍/㎏. In homologous passive cutaneous anaphylaxis(PCA) test using guinea pigs, inoculation of CFC-101 alone did not produce CFC-101-specific antibody. When inoculated with 200 ㎍/㎏ plus adjuvant, challenge of 200 ㎍/㎏ produced PCA titer of 32(5/5) but challenge of 20 ㎍/㎏ did not produce CFC-101-specific antibody. In heterologous PCA test using mice, CFC-101-specific antibody was not detected when sensitized with CFC-101 alone. Some animals(3/12) showed positive PCA response when inoculated with 200 ㎍/㎏ plus alum. In passive hemagglutination (PHA) test, although no antibody was detected at 20 ㎍/㎏, inoculation of 200 ㎍/㎏ alone or with alum produced positive response in all animals. This result has already been predicted because CFC-101 is a vaccine developed for the purpose of immunization. From the above results, it can be concluded that there is no adverse antigenic potential up to 10 times clinical dose of 200 ㎍/㎏.

Methamphetamine 이 B16 악성 흑색종 세포 전이에 미치는 영향

선우연(Woo Yearn Sun),한형미(Hyung Mee Han),신전수(Jeon Soo Shin),박현애(Hyeon Ae Park),정승태(Seung Tae Chung),김필선(Pil Sun Kim),손경희(Kyung Hee Sohn) 한국응용약물학회 1995 Biomolecules & Therapeutics(구 응용약물학회지) Vol.3 No.4

The effect of methamphetamine on the pulmonary metastasis was investigated in C57BL/6 mice injected with B16 melanoma cells. B16 melanoma cells (2x10^5 cells) were injected intravenously into 5∼7 weeks old C57BL/6 mice. Mice were then treated intraperitoneally with methamphetamine either acutely (two times with one week interval) or subchronically (daily for 14 days). Degree of pulmonary metastasis was investigated and specific immunologic parameters such as natural killer cell cytotoxicity(NKCC), antibody-dependent cellular cytotoxicity(ADCC) and blastogenic responses of splenocytes were examined. Mice which had been subchronically treated with methamphetamine showed significant decreases in the number of pulmonary metastasis of B16 melanoma cells, NKCC and ADCC without a significant change in blastogenic responses. In the acutely-treated group, slight trends of decrease in the numbers of pulmonary metastasis, NKCC and ADCC were observed without statistical significances whereas there was a significant increase in blastogenic responses. The mechanism underlying the decrease in the degree of metastasis despite diminished NKCC and ADCC after methamphetamine treatment and the relationship between the degree of pulmonary metastasis and duration of methamphetamine treatment remain to be investigated.

기니픽과 마우스에서 신규 퀴놀론 항균제 DW-116 의 항원성 시험

권현진(Hyun Jin Kwon),한형미(Hyung Mee Han),김필선(Pil Sun Kim),이흠숙(Heum Sook Lee),정용호(Yong Ho Chung),윤성준(Sung June Yoon),이문선(Moon Sun Lee),이덕근(Dug Keun Lee) 한국응용약물학회 1998 Biomolecules & Therapeutics(구 응용약물학회지) Vol.6 No.2

Antigenic potential of DW-116, a newly synthesized fluoroquinolone, was examined by conducting active systemic anaphylaxis (ASA), passive cutaneous anaphylaxis (PCA) and passive hemagglutination (PHA) tests. In ASA test, mild to moderate signs of anaphylactic responses were observed in the groups sensitized with low (2 mg/body) and high (10 mg/body) doses of DW-116 alone and the group sensitized with DW-116 plus adjuvant. Some moderate to severe anaphylactic reactions were observed in the group sensitized with a DW-116-bovine serum albumin (BSA) conjugate plus adjuvant when challenged with a DW116-guinea pig serum albumin (GSA) conjugate. However these reactions were considered to be a cross-reaction between BSA and GSA since similar reactions were induced when challenged by GSA alone. In heterologous PCA test using mice and rats, positive responses were not detected in any of the experimental groups. In PHA test, positive responses were observed in the groups sensitized with low and high doses of DW-116 alone and the group sensitized with DW-116 plus adjuvant. However, these responses were not considered to be drug-specific because some positive responses were also seen in the negative control group. From these results, it was concluded that DW-116 is not likely to have specific antigenic potential in clinical use.

Pesticide가 면역계에 미치는 영향에 관한 연구(Ⅱ) : Effects of methidathion and pyridaphenthion on the humoral immunity in mice

김형수,한형미,정혜주,김필선,박재현,김진호,정승태,윤여표,김주일 식품의약품안전청 1997 식품의약품안전청 연보 Vol.1 No.-

유기인계 농약인 methidathion과 p'fridaphenthion이 면역계에 미채는 영향을 살펴보고자 BALB/C 마우스에 저용량(0.5mg/kg)및 고용량 (5.0mg/kg)의 methidation 또는 저용량(4.6mg/kg) 및 고용량(46mg/kg)pyridaphenthion을 14일간 경구투여후 체중변화및 훙선등 면역관련장기의 무게 변화를 측정하고 면역적혈구에 대한 PFC측정 및 HEL에 대한 IgG항체가를 ELISA를 이용하여 측정하엿으며 조직병리학적 관찰을 행하엿다. 체중의 경우 methidathion 및 pyridaphenthion 고용량투여군에서 유의성있는 짬소를 나타냈으며, 면역관련장기의 경우 비장을 제외한 훙선, 신장, 간장의 상대적 무게를 유의성있게 증가도는 감소기켰으나 일관성있는 변화을 관찰할 수 없었더, PGG의 경우 methidathion및pyridaphenthion모든 투여군에서 비장당 및 비장 세ㅠㅗ당 프랄크형성능을 유의성있게 저하(p<0.001)시켰고, IgG항체가 측정의 경우에는 rnethidathioa 및 pyridapheuthion고용량군에서안 유의적 저하(p<0.05)를 관찰할 수 있었다. 조직병리학적관찰에서는 methidathion 및 pyridaphenthion 고용략틀 여군의 간과 신장조직에서 공히 세포변성이 나타났으며, 신장에서의 충출혈이 관찰되었다. 또한 홍선 및 비장조직에싻는 현저한 임파구 감소와 비장에서 거대핵세포가 관찰되었다. 특히 methidathion 투여군에서는 신장조직에서의 보운 만낭 위축 및 흥선에서의 충출혈 소견이 관찰되었다. 따라서 methidathion 및 pyridaphenthion은 체액성 면역능을 저하시키는 것으로 사료된다. The effects ()』 methidathion and pyridaphenthion on immune system were studied in BALB/C mice by assessment of body and organ weight, immune function tests like plaque forming eells(fFC) assar and engyme-linked immunesorbent assay (ELISA ) anfl histepathological eraminations.Methidathion (0.5,5.Omg/kg) and pyridaphenthioB (4.5,46mg/kg) wel·e given to mice orally for 14 daysand the each parameter waf evaluated on the day 15. Mice were immunized with sheep red blood cells(SRBC) on the day 11 for fFC assay and heE egg Iysozyme (HEL) was injected peritoneally on the day0 aBd 8 for ELTSA. Body we;ights were decreased signi(icantly (p<0.05) both in methidathion (5.Omg/kg) and pyridaphenthien (46mg/kg) treated grovps, but there was no consistency in changes of ralattveorgan weight, even though t;he weights of thymus, kidney and liver were increased or decreased signifi-cantly after treatment. The antibody response to SRBC was inhibited (p <0.001) in all pesticide-treatedgroups, whereas serum fge titer to HEL was only reduced (p<0.05) in methidathion (5.Omg/kg) andryridaphenthioa (46mg/kg) treated groups. Some abnormal changes in spleen, thymus, liver and kidneytissues w?fa: observed in methidathion(5.Omg/kg) and pyridaphenthion(46mg/kg) treated groups byhistopathological examiaations. These results suggest that methidathion and pyridaphenthion may sup-press the humoral immunity in mice.

치약중 불소성분에 관한 연구(Ⅱ) : 치약중 용성불소의 안정성 Stability of Soluble Fluoride in Toothpaste

최상숙,장영경,김인규,윤미옥,손경훈,김필선,이정표,정정자,오원식 식품의약품안전청 1998 식품의약품안전청 연보 Vol.2 No.-

치약에 치아우식증 둥을 예방하기 위하여 불소화합물이 많이 첨11되괴 있다. 치약에 주로 사용되는 불소화합물은 불화나트륨(보aF), 일불소인산나트륨(NaBPP03), 불화주석(SHf'B) 및 유기 툴소차합물이 사용되고 있다. 이들 성분은 치약중의 연마제 등 다른 성둘과 결합한 결합형 불소와 결합하지 쟈은 용성불소로 나뉘어 존재하며,생산시에는 대부분 용성불소의 상태로 존재하치만시간이 지남에 따라 결합헝 불소화함물로 바커어 활성을 잃게 되고, 그 정도는 치약중 연마제나 부형제의 종류와 양에 따라 영향을 받게 된다. 본 연구에서는 치약중 용성불소의 안정성에 미치는 연마제 및 첨가제와 포장재의 영향에 관하여 조사하여 그에 따라 simulated product의 처방플 설계하고, 불소화합물인 불화나트륨과 일뿔소인산나트륭, 연마제인 함수이산화규소, 알루미나, 인산일수소칼슘,침깡탄산칼슘 및 인산삶칼슘와 5종을 기본처방으로 한 18종의 치약을 제조하여 라미네이트 튜브와 폴리에칠렌 튜브에 각각 충진하였다. 이 검체를 가지고 제조 직후외 총불소 및 용성불소 함량을 측정하였고, 보관온도에 파른 용성불소 함량의 변화 및 실온 및 40'c서 3개월간 보환 후 파시 총불소 및 용성불쇼의 함량을 측정하여 용성불소의 안정성에 영향을 미치는 요인에 관하여 실험하였다. 정량은 전보에 수재한 이온크로마토그래피와 불소이온측정기에 의한 불화나트륨과 일불소인산나르륨좌 분석법을 이용하였다. 사험한 결과 제조 직후에는 연마제 인산삼칼슘을 사용한 3종의 치약은 모두 용성불소함량이 40%이하로 현저히 저하죄었파.또한, 연마제의 종류와 상관얼이 전반적으로 불화나트륨을 사웅한 처방이 일불소인산나트륨을 사용한 처방보다 용성불소랑이 더 낮게 나타났다. 3개월후의 검체를 시험한 결과 상온에 보관한 검체는 생산 직후의 결과와 크게 다르지 않았고 튜브의 종류에 따른 차이는 나타나지 않았다. 그러나 40'c에서 보관한 검체는용쳐불소의 양이 전반적으로 현저히 감소하였오 감소.정도는 연마제의 종류에 따라 영향을 받는 것으로 나타났으며 튜브의 종류에 따른 차이는 나타나지 않았다. 이상의 결과로 불소치약의 연마제로 인산삼칸습은 T'☞우 부작합하였고, 함수이산화규소 또는 알루미 나는 비교적 양호한 결과를 나타내었다. 튜브의 종류는 용성불소의 안정성에 별 놓향을 미치지 않았으나 40'c에서 3개월간 가속시험할 때 전반적으로 불쇼는 연마제 등 다른 성분과 더욱 강하게 결합하는 것을 알 수 있었다. In fluoride toothpaste, sodium fluoride, sodium monofluorophosBhate or stanBous flue. ride are usually used as fluoride donor. These flouride compounds may react with abrasive agents so thesoluble fluoride ion content and its acitivity are decrf:aaed. In this gtudy, we prepared fluoride toothpasteaccording to simulated formulation with fluoride compound(sodium inonofluorophosphate, sodium flufride), abrasiues(hydrated silica, alumina, dibasic calciunl phosphate, precipitated calcium carbonate,kind of the abrasives, fluoride compounds, pac]fags naaterials and variation of temperatvre. The soluble fluorde ion content was stable when using hydrated silica as abrasive. But, tricalcium phosphate wasnot suitable in fluoride toothpaste as abrasive. The soluble fluoride ion content was decreased as raisiBgtemperature and not influenced by package materials.