PCR을 이용한 Rat 기관지 세척액에서의 Pneumocystis carinii DNA의 검출 : 기초실험 A pilot study

김주옥,홍석철,한표성,이종진,김선영,이영하,신대환 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.1

Background: Pneumocystis carinii (PC) is a opportunistic pathogen causing serious pneumonia (Pneumocystis carinii pneumonia : PCP) in immunosuppressed patients including AIDS. Laboratory diagnosis of PCP is dependent on microscopic demonstration of the PC by using cytochemical staining or by immunocytochemical staining with monoclonal antibodies. However, these staining methods are not highly sensitive. The development of the polymerase chain reaction (PCR) has made it possible to detect very small numbers of pathogens in clinical specimens. To establish the usefulness of PCR for detection of PC DNA, We performed PCR as a pilot study. Methods: PCP was induced on Spraque-Dawley rats with prednisolone 5mg IM twice a week. Bronchial lavages, impression smears, and permanent sections were performed from the 3rd week to 7th week of cortisonized rats. PC DNA was extracted with bead beater / 10% CTAB method. Results: Impression smears showed PC cyst after the 4th week (100%, 28/28 rats), and permanent sections showed 27/28 rats. Control rats showed some PC cysts (2/7). PCR result was positive only one case among the 6th week rats (1/7), But positive 6 cases at the 7th week rats, (6/7). Among control rats. 2 cases were positive (2/7). ??This data is a pilot study for the PC DNA detection using a PCR. PCP were successfully cortisonized rats. If adequate bronchial washing technique will be used, PCR can be a sensitive for PC DNA detection.

Interventional Pulmonology : Photodynamic Therapy(PDT) in Lung Cancer

김주옥,정미경 대한결핵 및 호흡기학회 2006 워크샵 Vol.24 No.-

급성 세균성 감염증에 대한 Cefotetan(Yamatetan^ⓡ)의 임상적 효과

김주옥,안원전,박종훈,노흥규,박종우 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.1

Total 20 cases of infectious diseases were treated with Cefotetan at the Dept. of internal medicine, Chung Nam National University Hospital, Dae Jeon, Korea, from January 1985 to April 1985. Among 20 cases, 6 were the patients with acute pyelonephritis, 7 were pneumonia, 2 were acute cholecystitis, 2 were chronic bronchitis, 2 were cellulitis and 1 was lung abscess. The response was good in 16 cases, and poor in 4 cases. The laboratory tests were normal except I which was reversible. (mild elevation of SGOT and SGPT) The side effects were noted in 2 cases, such as skin eruption and drug fever, which were improved with supportive care. Cefotetan was thought to be useful against acute bacterial infections of respiratory, urinary, biliary tracts and cutaneous tissue.

객혈의 임상적 고찰 및 기관지 내시경의 역할

김주옥,이종진,서지원,김선영 충남대학교 의과대학 지역사회의학연구소 1995 충남의대잡지 Vol.22 No.2

118 patients with hemoptysis were reviewed retrospectively to identify etiologic factors, clinical features, and treatment and to evaluate the usefulness of fiberoptic bronchoscopy. 63 cases (53.4%) were blood-tinged sputum, 2 cases (1.7%) were more than 600ml a day in amount of hemoptysis. Pulmonary tuberculosis was 37 cases (31.36%), the most common cause of the known etiologic factors. Lung cancer was 6 cases (5.09%). 6 patients (5.09%) were treated with bronchial artery embolization, 1 patient (0.85%) treated with right lower lobectomy. 1 (4.3%) of 23 patients with hemoptysis and normal chest roentgenogram was found to have lung cancer. We suggest that in patients with hemoptysis and normal chest roentgenogram, routine fiberoptic bronchoscopy is not necessary to exclude malignancy.

폐암환자에 있어서 T 세포 매개성 면역의 변화(Ⅰ)

김주옥,김선영 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.2

Cell-mediated immunity (CMI) was evaluated in 30 patients with lung cancer by in vivo and in vitro methods. These included skin test with CMI multitest kit, determination of peripheral T lymphocytes, and lymphocyte response to phytohemagglutinin (PHA) and concanavalin A (Con A). The positive scores of skin test were significantly lower in lung cancer group than controls (p<0.05), and there were no significant differences in T cell subsets. The response to PHA and Con A were greatly depressed in lung cancer and it was more apparent in advanced stage. But there was no difference in stimulation index (SI) between cancer patients and normal controls. The cumulative index (Cl) was more significant difference in cancer patients and advanced stage. These results suggest that depressed CMI in patients with lung cancer might be caused by lymphocytic dysfunction and cumulative index is more accurate to describe lymphocyte blastogenic function than stimulation index.

결핵환자에 있어서 세포성 면역의 변화 (1)

김주옥,김선영 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.2

It is well established that cell-mediated immunity including T cell activation is the most important mechanism for the host defence against Mycobacterium tuberculosis. There are some indices for describing the mitogen-induced lymphocyte transformation such as stimulation index (SI). Recently curnmulative index (CI) was suggested to describe lymphocyte blastogenic function more accurately. To evaluate the abnormalities of cell-mediated immunity and usefulness of CI, we measured the T cell subsets and compared the indices (SI and CI) of lymphocyte transformation of peripheral mononuclear cells in patients with tuberculous patients (17 with pulmonary tuberculosis, 5 with tuberculous pleurisy). The postitive scores of skin test using CMI multitest kit were significantly decreased in patients group compared to normal controls. The percentage of total T lymphocytes in patients was not significantly different from normal controls, but that of helper T cells was significantly decreased and that of suppressor T cells was significantly increased, resulting significant decrease in T_4/T_8 ratio in patients group. The SI in patients was not significantly different from controls because of wide standard deviation, but CT was significantly decreased in patients group. As a result of this study, we could confirm the abnormal cell-mediated immunity in tuberculous patients and suggest that CI is a more useful parameter for describing the mitogen-induced lymphocyte transformation.

장애인활동지원서비스의 선택 제약에 관한 연구: 발달장애아동 부모의 서비스 이용 패턴을 중심으로

김주옥,김수영 한국사회복지학회 2023 한국사회복지학 Vol.75 No.3

This study aims to reveal the structures that restrict service choice in the utilization pattern of parents of children with developmental disabilities(DD) who choose and utilize Personal Assistance Service(PAS). In-depth interviews were conducted with 10 parents who have utilized the service, and the results were analyzed using a pattern matching method. As a result of the analysis, parents of children with DD did not take the initiative in accessing services, choosing PAS assistants and service agencies, and raising objections, contrary to the official service utilization patterns. Theses constraints in service choice were caused by a screening process that failed to adequately consider the characteristics of DD, PAS assistants who solely existed as service stakeholders, and service agencies that received fees from both users and PAS assistants. Furthermore, a constraint structure in service choice was also influenced by parents who lacked understanding of the purpose of the service. Based on these findings, this paper suggested political and practical recommendations that can incorporate the needs of children with DD throughout the PAS utilization process. 본 연구는 발달장애아동 부모가 활동지원서비스를 이용하는 패턴을 살펴봄으로써 서비스 선택에 제약을 받는 구조를 드러내는 데 목적이 있다. 이를 위해 활동지원서비스 이용 경험이 있는 부모 10인에게 심층인터뷰를 진행하여, 서비스 이용 과정에서 제약이 발생하는 지점과 제약을 발생시키는 요인에 대해 패턴매칭 방법을 사용하여 분석하였다. 분석 결과, 발달장애아동의 부모는 공식적인 서비스 이용 흐름 절차와 달리 서비스 진입을 위한 신청과정, 제공기관 및 제공인력과의 계약과정에 있어 주도적으로 선택권을 행사하지 못했으며, 서비스 이의 신청 또한 이용자의 선택이 아닌 제공인력의 요청에 의해 이루어지고 있었다. 이러한 서비스 선택의 제약 구조는 발달장애 특성을 제대로 반영하지 못하는 심사과정, 사회서비스를 자신의 일로만 보는 제공인력, 이용자와 제공인력 사이에서 수수료를 받게 되는 중개기관에 의해 발생하고 있었다. 또한 서비스 목적에 대한 이해가 부족한 부모에 의해서도 서비스 선택의 제약 구조가 발생하기도 하였다. 본 논문은 위 결과를 토대로 활동지원서비스 이용 과정 전반에서 발달장애아동의 욕구를 반영할 수 있는 정책적·실천적 제언을 하고자 한다.

현대미술에서 보이는 비인간 개체의 행위성

김주옥 예술과미디어학회 2023 예술과 미디어 Vol.22 No.2

This study discusses ‘(non)humanism’ and agency that can be seen in the context of ‘posthumanism’ through the example of exhibition curating and seeks to understand what ‘subjectivity’ means. The interest in posthumanism appeared in the movement to break away from anthropocentrism and avoid dualistic thinking. In the meantime, we have experienced the entry of the thinking of ‘New Materialism’, represented by ‘Actor-Network Theory’ of Bruno Latour(1947-2022). Since not only humans but also nonhumans have the same agency as humans, humans and nonhumans have a symmetrical composition. On the extension of this reasoning, we now need a broader thought on (non)humans, not on the opposite side of humans. In order to think about (non)humans who are not humans and nonhumans, we first need to pay attention to the perspective of ‘non’ in the word ‘nonhumans’ that we have made others. This study aims to analyze and organize this thought through curatorial research and works of art. And this analysis of works has the goal of reminding us of the subjectivity of nonhumans revealed in the works being experimented with through contemporary art and presenting how the agency created by these nonhuman entities is realized. And the significance of the study that can be had through this is to imagine the post(non)human realm by discussing the postnonhuman imaginable through the context of posthumanism.

기관지경 생검상 확진된 폐암환자에서 기관지세척 세포진과 다른 지표들과의 관계

김주옥,서지원 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.2

Bronchoscopic examination is an essential tool for diagnosis and treatment of the lung cancer. Various method with bronchoscopy are possible, such as cytologic examination (washing, brushing, bronchoalveolar lavage and transbronchial needle aspiration0, mucosal biopsy and transbronchial lung biopsy. There are many reports on the bronchial washing cytology, but there were few reports on the relations between bronchial washing cytology and other various parameters(age, sex, celltype, bronchoscopic location and shape of lesion). To evaluate the relationships between washing cytologic yield, age, sex, celltype, bronchoscopic location and shape of lesion in lung cancer, we have studied in 189 patients(males 170, females 19; ages, mean 60.7yrs) with histologically proven lung cancer who showed direct signs of lung cancer bronschoscopically. All cases were collected washing cytology specimens simultaneously. :There were no significant relationship between age and washing cytology positivity, celltype or bronchoscopic shape of lesion, between sex and washing cytology positivity, bronchoscopic location or shape of lesion, There was significant relationship between sex and celltype, that is, NSCLC in male vs SCLC in female. There were no significant relationship between cytology positivity and celltype, bronchoscopic location or shape of lesion. There was significant relationship between celltype and bronchoscopic location of lesion, that is, squamous and SCLS in both upper lobes va adenocarcinoma in RLL. There was no significant relationship between bronshoscopic location and shape of lesion, but significant between celltype and shape of lesion.(mass in squamous vs non-mass in SCLC). In conclusion, lung cancer has more prevalent in older males than females and both upper lobes, and squamous cell carcinoma has more mass-shaped and SCLS has more infiltrated lesion bronchoscopically. The cytologic positivity was not depended on the bronchoscopic shape of lesion.

발달장애인의 미래계획은 앞으로 어떻게 가야 하는가? : 발달장애 당사자와 가족의 자립의지를 중심으로

김주옥,임예직 한국사회복지학회 2023 한국사회복지학회 학술대회 자료집 Vol.2023 No.10