방광암 병기결정에 배설성 요로조영술의 가치

김원재,이상은,김영균 大韓泌尿器科學會 1987 Korean Journal of Urology Vol.28 No.1

Can We Use Single Nucleotide Polymorphism and Runt Domain Transcription Factor 3 Methylation as Tumor Markers for Bladder Cancer?

김원재,김용준 대한비뇨의학회 2009 Investigative and Clinical Urology Vol.50 No.4

Purpose: Although many tumor markers have been evaluated in relation to bladder cancer, none of these biomarkers reported to date has shown sufficient sensitivity and specificity for the detection of the whole spectrum of bladder cancer diseases in routine clinical practice. The limited value of the established prognostic markers requires analysis of new molecular parameters of interest for predicting the prognosis of bladder cancer patients. Materials and Methods: We conducted a review of the literature with a focus on recent advances in genetic polymorphism and hypermethylation events in relation to bladder transitional cell carcinoma. Results: Recently, there has been major progress in both genetic polymorphism in relation to bladder cancer and molecular genetic and epigenetic changes leading to the development of transitional cell carcinoma. However, studies on numerous single-nucleotide polymorphisms in relation to bladder cancer have provided only a few genetic polymorphisms with only marginal information on patients’ prognosis. For this reason, interest is increasing in epigenetic changes in bladder cancer. The epigenetic silencing of tumor suppressor genes is interesting from a clinical standpoint because of the possibility to reverse the epigenetic changes and thus restore gene function to a cell. Treatment with DNA methylation inhibitors can restore the activities of dormant genes and decrease the growth rate of cancer cells in a heritable fashion. Conclusions: Epigenetic modification may be possible to partially reverse the cancer phenotype, and this will eventually lead to targeted therapy tailored toward specific molecular therapy in the near future. Purpose: Although many tumor markers have been evaluated in relation to bladder cancer, none of these biomarkers reported to date has shown sufficient sensitivity and specificity for the detection of the whole spectrum of bladder cancer diseases in routine clinical practice. The limited value of the established prognostic markers requires analysis of new molecular parameters of interest for predicting the prognosis of bladder cancer patients. Materials and Methods: We conducted a review of the literature with a focus on recent advances in genetic polymorphism and hypermethylation events in relation to bladder transitional cell carcinoma. Results: Recently, there has been major progress in both genetic polymorphism in relation to bladder cancer and molecular genetic and epigenetic changes leading to the development of transitional cell carcinoma. However, studies on numerous single-nucleotide polymorphisms in relation to bladder cancer have provided only a few genetic polymorphisms with only marginal information on patients’ prognosis. For this reason, interest is increasing in epigenetic changes in bladder cancer. The epigenetic silencing of tumor suppressor genes is interesting from a clinical standpoint because of the possibility to reverse the epigenetic changes and thus restore gene function to a cell. Treatment with DNA methylation inhibitors can restore the activities of dormant genes and decrease the growth rate of cancer cells in a heritable fashion. Conclusions: Epigenetic modification may be possible to partially reverse the cancer phenotype, and this will eventually lead to targeted therapy tailored toward specific molecular therapy in the near future.

지도자 인식 기반 초등학교 태권도 겨루기 선수들의 경기력 향상 방안

김원재,이윤수 국기원 2019 국기원태권도연구 Vol.10 No.3

The purpose of this study was to provide implications to the elementary school Taekwondo instructors by exploring improvement plans for Taekwondo Kuyorugi performance based on the perception of instructors. To achieve this goal, six instructors were selected as research participants using a purposeful sampling method. The literature data including relevant prior studies were collected first, and the in-depth interview was conducted more than once by each research participant with semi-structured questionnaires to collect data. Researchers also observed the scene of instructors’ Taekwondo training process to their children through non-participant observation. These collected and analyzed data are categorized and the categorized results are as follows: The three main factors affecting Taekwondo performance were instructor factors, environmental factors, and technical factors. In detail, the instructor factors were categorized into three categories: education through goal setting and motivation, utilization and advantages of instructors’ experience, and the unstoppable efforts of instructors. The environmental factors were categorized into two categories: the changed operation of the competition caused by the introduction of electronic protective gears and the performance by whether or not to use the electronic protective gears during training. Finally, the technical factors were categorized into two categories: the changed kick, and reduced step and motion, and the risk of turning kick. Based on the results, considering each categorized factor, the instructors of elementary school Taekwondo Kyorugi competitions should perform appropriate coaching activities to help improve their Taekwondo athletes' performance. 본 연구는 지도자의 인식을 기반으로 초등학교 태권도 겨루기 선수들의 경기력 향상 방안을 탐색함으로써 초등학교 태권도 지도자들에게 시사점을 제공하는데 목적이 있다. 이러한 목적을 달성하기 위해 유목적 표집방법을 사용하여 현직 지도자 6명을 연구참여자로 선정하였다. 먼저 관련 선행연구를 포함한 문헌자료를 수집하였고, 반구조화된 질문지를 가지고 연구 참여자 별 심층면담을 2차례 이상 실시하여 자료를 수집하였다. 또한 연구자가 직접 지도자들이 초등학생 태권도 훈련을 시키는 장면을 비참여관찰을 통해 관찰하였다. 이렇게 수집되고 분석된 자료들을 범주화 하였으며 그 범주화된 결과는 다음과 같다. 태권도 경기력에 미치는 요인을 크게 지도자요인, 환경요인, 그리고 기술요인 세 가지로 범주화하였다. 세부적으로 지도자요인으로는 목표설정과 동기부여를 통한 교육, 지도자 경험들의 활용과 장점, 그리고 멈추지 않는 지도자들의 노력의 세 가지로 범주화하였다. 환경요인으로는 전자호구 도입으로 인한 변화된 경기운영, 훈련 시 전자호구 사용여부에 따른 경기력의 두 가지로 범주화하였다. 마지막으로 기술요인으로는 변화된 발차기와 줄어든 스텝과 모션, 회전발차기의 위험성의 두 가지로 범주화하였다. 이러한 결과를 바탕으로 초등학교 태권도 겨루기 지도자들이 선수들을 지도할 때 각각의 범주화된 요인들에 관한 내용을 고려하여 선수들의 경기력 향상을 위하여 적절한 지도를 해야 할 것이다.

Wernicke’s Encephalopathy Presenting with Bilateral Complete Horizontal and Downward Gaze Palsy in a Malnourished Patient

김원재,김명미 대한안과학회 2017 Korean Journal of Ophthalmology Vol.31 No.4

'디지털 삶'과 '체험소비'에 관한 단상

김원재,Kim, Won-Jae 대한인쇄문화협회 2003 프린팅코리아 Vol.7 No.-

한강수계 상수원 수질특성별 활성탄 및 오존/AOP 공정의 운전조건 최적화 연구

김원재,서재승,강미성,오현제,정문경,오성민 대한환경공학회 1998 대한환경공학회 학술발표논문집 Vol.1998 No.12

아스팔트 소성변형 모형의 개발 및 보정

김원재,이현종,르반푹,판 후이 티엔,이진욱,김태우 한국도로학회 2016 한국도로학회 학술발표회 논문집 Vol.2016 No.06

A permanent deformation model for asphalt concrete (AC) layers based on shear properties is developed and calibrated in this study. The indirect tensile (IDT) strength and uniaxial compressive strength (UCS) were used to determine the cohesion and friction angle of asphalt mixtures. Five types of asphalt mixtures with various air-void and binder contents are tested under different loadings and temperature conditions. Multiple regression analysis was conducted to develop permanent deformation model of asphalt mixtures which is a function of the air void, binder content, stress, and number of load cycles, temperature, and frequency. The regression model was verified by comparing the predicted and measured plastic strain. It was found out that the regression model has a correlation coefficient of 0.96 in determining plastic strain. Through the field WesTrack database, the permanent deformation model was calibrated to be applied to AC layers. After calibration, it is observed that the correlation coefficient is 0.86 for the measured and predicted rut depth. Finally, the model is validated using the field rutting performance obtained from Long Term Pavement Performance (LTPP) data sets. Based on the validation study, it can be concluded that the proposed model can successfully predict the permanent deformation of various asphalt mixtures in a wide range of loadings and temperature conditions in the field.

사람 치은 섬유모세포에서 Cyclosporin-A 유도 세포증식에 대한 항세포고사 기전

김원재,정현주,정태술 대한치주과학회 2005 Journal of Periodontal & Implant Science Vol.35 No.3

Cyclosproin A(CsA)는 세포 이식거부방지를 위한 면역 억제제 및 자가 면역질환 치료제로 널리 사용되어 왔다. CsA는 배양된 사람 치은섬유아세포를 증식시킴이 알려져 있지만 CsA에 의한 세포증식기전에 대한 세포사멸기전 및 Bcl-2의 역할은 연구되어 있지 않다. 이번 연구는 사람 섬유아세포에서 CsA에 의한 세포증식기전에 세포고사기전 및 Bcl-2 family가 관여하는지 밝히는 데에 목적이 있다. 세포 생장력은 MTT 방법으로 측정하였다. Bcl-2 family와 Fas 발현 정도는 RT-PCR 방법이나 western blot으로 확인하였다. Caspase-3 및 -9의 활성은 ELISER reader로, reactive oxygen species(ROS)는 fluorescence spectrometer에 의해 측정되었다. 미토콘드리아에서 세포질로 분비된 cytochrome c는 Western blot으로 조사하였다. CsA는 0.1~10 μM에서 사람 섬유아세포의 생존률을 시간과 농도 의존적으로 증가시켰으며, 50 μM CsA에서는 오히려 세포가 죽었다. 또한, CsA 처리로 미토콘드리아에서 세포질로 유리되는 cytochrome c 양과 VDAC 1 및 3 발현량이 감소되었고, caspase-9과 caspase-3의 활성도도 감소되었다. 한편, CsA 처리한 섬유아세포에서 death receptor 구성요소인 Fas 발현이 감소되었다. Bcl-2 family에 대한 RT-PCR, western blot 분석결과, 세포고사를 억제하는 Bcl-2 발현은 증가되었으나 세포고사를 자극하는 Bax와 Bid의 발현은 감소되었다. 이러한 결과들은 사람 섬유아세포에서 CsA유도 세포증식에 Bcl-2 family와 ROS가 매개하는 미토콘드리아 의존 및 death receptor 의존 세포고사기전이 관여함을 시사하였다.

4차 산업혁명 ‘디지털트윈’기술의 동향과 군 활용방안

김원재,박용찬,정용균,최영도,전석춘 한국방위산업진흥회 2021 國防과 技術 Vol.- No.513

Knowledge-based diagnosis and prediction using big data and deep learning in precision medicine

김원재 대한비뇨의학회 2018 Investigative and Clinical Urology Vol.59 No.3