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기관지 내시경으로 초기에 제거할 수 없었던 기도 이물 : 2례 보고
김연수,남승연,곽병곤,장우익,박경택,김창영,류지윤,Kim, Yeon-Soo,Nam, Seung-Yeon,Kwak, Byeong-Gon,Chang, Woo-Ik,Park, Kyung-Taek,Kim, Chang-Young,Ryoo, Ji-Yoon 대한기관식도과학회 2007 大韓氣管食道科學會誌 Vol.13 No.2
Foreign body aspiration is a cause of the accidental death at home. Therefore, early intervention and proper management is important. A bronchoscopy is indicated whenever there is a suggestive history and medical opinion. Occasionally, foreign body removal with bronchoscopy may be fail. But, on the situation, there is no definite recommended standard management. We experienced two cases of bronchial foreign body could not be removed with bronchoscopy at first intervention. The one was diagnosed too late. Endobronchial granulation tissue and edema made it impossible to find the foreign body at first bronchoscopy. After steroid and antibiotic therapy, foreign body could be removed with secondary bronchoscopy. Another was bronchial foreign body jammed tightly bronchus intermedius. Even after medical therapy, patient got aggravated. So foreign body was removed with bronchotomy.
치안분야의 정보통신기술 활용방안 연구 - 빅데이터기반 치안수요분석과 대응체계를 중심으로 -
김연수,Kim, Yeon Soo 한국융합보안학회 2016 융합보안 논문지 Vol.16 No.6
급격한 정보통신기술의 발달은 경찰에게 새로운 도전이자 기회가 되고 있다. 이 연구는 국내외 치안분야의 ICT기술 활용사례를 검토하고, 그 시사점을 확인하는 것을 목적으로 한다. 이를 위해 정보통신기술 활용에 관한 경찰활동의 근거이론으로 정보주도 경찰활동(intelligence-led policing), 예측적 경찰활동(predictive policing), 증거기반 경찰활동(evidence-based policing)의 개념에 대해 소개하였다. 또, 빅데이터 기반 범죄분석과 예측기술 활용방안과 경찰의 정보통신체계 및 지휘통제기술의 고도화방안에 대해 논의하였다. 이 연구를 통해 확인한 시사점으로 (1) 기본데이터의 확보, (2) 활용가능한 통합D/B의 구축, (3) 정책결정자의 활용성 증대, (4) 유관기관 사이의 교류협력, (5) 전문가집단의 양성, (6) 통합 D/B구축의 법적 근거와 실무적 가이드라인 마련을 제안한다. Rapid advances in information and communications technology are new challenges and also opportunities for the police. For the purpose of identifying its implications, this study reviews utilization cases of information and communications technology in the field of public security in South Korea and other countries. As theoretical basis for utilization of information and communications technology, this study introduces intelligence-led policing, predictive policing and evidence-based policing. Also, utilization of big-data based crime analysis and crime prediction technology, as well as advancement of information and communications system and command and control technology of the police, are discussed. Based on the identified implications in this study, the following proposals are made. They are (1) procuring basic data, (2) creating an integrated database, (3) increasing utilization of policy decision-makers, (4) exchange and cooperation between related institutions, (5) training professional analyzers, (6) establishing legal basis and practical guidelines for an integrated database.
Hepatitis B Virus의 표면항원 유전자의 Cloning과 발현에 관하여
김연수,강현삼,Kim, Yeon-Soo,Kang, Hyen-Sam 생화학분자생물학회 1984 한국생화학회지 Vol.17 No.1
사람의 피로부터 hepatitis B virus(HBV)를 순수 분리한 뒤 endogenous DNA polymerase 반응에 의해 완전한 double strand HBV DNA를 얻었다. 이 circular double stranded DNA를 BamHI으로 절단한 둬 pBR322에 삽입시켜 HBV의 total genome을 포함하는 재조합 plasmid pHBV-315를 제조하였다. cloning된 viral genome을 분석하기 위해 pHBV-315의 제한효소 지도를 작성하고 일부 염기 서열을 비교 조사하였다. 이 결과로부터 subtype adr HBsAg 유전자가 포함된 HBV DNA가 cloning 되었음을 알 수 있었다. pHBV-315로 부터 pHBV-15를 재조합하여 LMTK-세포와 E. coli에서 HBsAg 유전자의 발현을 조사하였다. 그 결과 LMTK-세포에서는 HBsAg이 검출되었고 E. coli에서는 검출되지 않았다. Dane particles were purified from human plasma and the DNA containing the single strand region was filled-in by endogenous DNA polymerase. Recombinant plasmid pHBV-315 was constructed by inserting the total HBV DNA into pBR322 DNA. Restriction enzyme map and nucleotide sequence of pHBV-315 suggested that the HBsAg subtype of the cloned HBV DNA is adr and how HBsAg gene is arranged in pHBV -315. pHBV -15 was constructed to direct the expression of HBsAg gene. When LMTK-cells and E.coli were transformed by pHBV-15, HBsAg was produced in LMTK-cells but not in E.coli.
김연수,박경택,류지윤,김창영,장우익,장선희,Kim, Yeon-Soo,Park, Kyoung-Taek,Ryoo, Ji-Yoon,Kim, Chang-Young,Chang, Woo-Ik,Chang, Sun-Hee The Korean Bronchoesophagological Society 2008 大韓氣管食道科學會誌 Vol.14 No.2
Nerogenic tumor of various histologic types may arise in the posterior mediastinum. Mediastinal schwannoma is a frequent paraspinal neurogenic tumor, but malignant mediastinal schwannoma is rare tumor which is derived from Schwann cells. Although there are some reports dealing with approach for screening patients with symptoms suggesting malignancy and the imaging criteria for distinguishing malignant from benign schwannoma but the results are not clearly defined. We present a case of hugh mediastinal schwannoma which was taken for malignancy in imaging studies because of its invasiveness.
좌측 주기관지 근위부에서 발생한 무기폐를 동반한 횡문근 육종의 수술 치료 -1례 보고-
김연수,장우익,허진원,박시영,장선희,박경택,김창영,류지윤,조성준,Kim, Yeon-Soo,Chang, Woo-Ik,Huh, Jin-Won,Park, See-Young,Chang, Sun-Hee,Park, Kyung-Taek,Kim, Chang-Young,Ryoo, Ji-Yoon,Cho, Seong-Joon 대한기관식도과학회 2007 大韓氣管食道科學會誌 Vol.13 No.2
Treatment choice for primary pulmonary sarcoma is complete surgical resection. A 69 year old man developed dyspnea due to left lung atelectasis. There was endobronchial tumor completely obstructing the left main bronchus. The tumor was resected completely by main bronchial resection via a left thoracotomy incision, and diagnosed as leiomyosarcoma. Bronchoscopy and computed tomography in 6 months after operation, there was no evidence of recurrence.
Functions of Early Palindrome Domain in SV40 DNA replication
김연수,하정실,강현삼,Kim, Yeon-Soo,Ha, Jeong-Sil,Kang, Hyen-Sam 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.3
SV40 Replication core origin에 존재하는 early palindrome의 구조와 기능에 대해 알아보기 위해 SV40 origin을 포함하는 0.8kb DNA fragment를 filamentous phage M13 DNA에 cloning시킨 후 oligonucleotide-directed site-specific mutagenesis에 의해 early palindrome이 이룰 수 있는 hairpin 구조 중 stem 부분이 파괴된 mutant들과 base의 치환은 있으나 stem 부분은 다시 회복된 mutant들을 만들었다 in vivo replication assay를 위해 mutation이 일어난 origin 부위를 pAT153 vector로 subcloning하여 COS-1 cell에 transfection함으로써 replication level을 측정하였다. 그 결과 early domain의 inverted repeats는 fuctional cruciform structure를 형성함으로써 SV40 replication에 관여하는 것은 아니라는 것을 알 수 있었다. To define the roles of the early palindrome domain within the SV40 replication origin region involved in initiation of DNA synthesis, 800-bp DNA fragment containing the simian virus 40(SV40) origin of replication was inserted into filamentous phage M13 DNA. Four kinds of mutants were introduced into this recombinant phage DNA, M13SV-2, by oligonucleotide-directed site-specific mutagenesis. Then, the origin fragments containing one or two base substitutions were subcloned into pAT153 vector, a derivative of pBR322. In two mutants (pATSV-A,-B) the stems were destroyed by single base substitutions. In the other two mutants(pATSV-C,-D) the normal stems were restored by additional base substitutions which complements the bases altered initially. While pATSV-A and pATSV-C could tolerate base substitutions, pATSV-B and pATSV-D showed dramatic decrease in DNA replication.
소 성장호르몬 cDNA 및 유전자의 분리와 대장균에서 cDNA 의 발현
김연수,정신우,김병한,이명희,설동섭,강현삼 ( Yeon Soo Kim,Shin Wu Jeong,Byung Han Kim,Myung Hee Lee,Dong Sub Sul,Hyen Sam Kang ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.1
cDNA was prepared from poly(A)^+mRNA isolated from pituitaries and cloned in E. coli. It contains the coding sequence for bovine growth hormone(bGH), The cloned bGH cDNA was confirmed by colony hybridization, restriction enzyme mapping, and nucleotide sequencing. The cloned eDNA was modified to construct the plasmid that expresses the bovine growth hormone in bacteria. A genomic gene of bovine growth hormone was also cloned using the bGH cDNA as a probe.
Hepatitis B Virus 의 표면항원 유전자의 Cloning 과 발현에 관하여
김연수,강현삼 ( Yeon Soo Kim,Hyen Sam Kang ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.1
Dane particles were purified from human plasma and the DNA containing the single strand region was filled-in by endogenous DNA polymerase. Recombinant plasmid pHBV-315 was constructed by inserting the total HBV DNA into pBR322 DNA. Restriction enzyme map and nucleotide sequence of pHBV-315 suggested that the HBsAg subtype of the cloned HBV DNA is adr and how HBsAg gene is arranged in pHBV-315. pHBV-15 was constructed to direct the expression of HBsAg gene. When LMTK-cells and E.coli were transformed by pHBV-15, HBsAg was produced in LMTK-cells but not in E.coli.
Simian Virus 40 복제 원점 부위의 특정 염기서열의 기능
김연수,하정실,강현삼 ( Yeon Soo Kim,Jeong Sil Ha,Hyen Sam Kang ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.3
To define the roles of the early palindrome domain within the SV40 replication origin region involved in initiation of DNA synthesis, 800-bp DNA fragment containing the simian virus 40(SV40) origin of replication was inserted into filamentous phage M13 DNA. Four kinds of mutants were introduced into this recombinant phage DNA, M13SV-2, by oligonucleotide-directed site-specific mutagenesis. Then, the origin fragments containing one or two base substitutions were subcloned into pAT153 vector, a derivative of pBR322. In two mutants (pATSV-A,-B) the stems were destroyed by single base substitutions. In the other two mutants(pATSV-C,-D) the normal stems were restored by additional base substitutions which complements the bases altered initially. While pATSV-A and pATSV-C could tolerate base substitutions, pATSV-B and pATSV-D showed dramatic decrease in DNA replication.
DNA Amplified Fingerprinting 기법을 이용한 Salmonella pullorum과 Salmonella gallinarum의 다형성 비교 분석
김연수,김상균,송원철,황의경,Kim, Yeon-Soo,Kim, Sang-Kyun,Song, Won-Chul,Hwang, Eui-Kyung 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.3
This study was performed to detect the Salmonella genus-specific DNA marker for comparing of polymophisms between S pullorum and S gallinarum by using PCR amplified techniques. A total of ten primers were used to detect DNA polymorphisms from S pullorum and S gallinarum. The number of DAF bands detected per each primer varied from 26 to 45, with an average of 32.7 using 10 primers. A total of 327 DAF bands were generated and among them 123 bands were polymorphic(37.6%). These DNA amplified fingerprinting(DAF) specific bands for S pullorum and S gallinarum were observed from all primers. For S pullorum, GEN 60-04, GEN 70-04 and GEN 70-03 primers showed a high level of polymorphism with 0.79, 0.70 and 0.57, respectively. But GEN 60-05 primer did not show a level of polymorphism. For S gallinarum, GEN 70-03, 60-04, 60-07, 70-05 and 70-04 primers showed a higher a low level of polymorphism from 0.16 to 0.28. Each five strains of S pullorum and S gallinarum were isolated from chickens showed typical clinical signs related with infection of pullorum disease or fowl typhoid at commercial chicken farms. DNA markers of these strains produced by GEN 70-04, GEN 70-05 and GEN 70-08 showed significant difference of band patterns between S pullorum and S gallinarum. These DNA markers could be used for comparison of DNA marker polymorphism between S pullorum and S gallinarum as well as rapid diagnosis of fowl typhoid and pullorum disease of domestic fowls.