계측분석을 위한 연약지반 거동의 이해

김상환,김상환 대한지질공학회 2003 지질공학 단기강좌 Vol.2003 No.-

Effect of Extenders with TCG and DMSO on the Viability of Rabbit Sperm

김상환,어열,방성규,오민지,박찬희,윤종택 사단법인 한국동물생명공학회 2019 한국동물생명공학회지 Vol.34 No.2

The purpose of this study was to evaluate the effect of addition of ethylene glycol, glycerol and sucrose to TCG (Tris, Citric Acid, Glucose, Egg Yolk) and DMSO Frozen. The extender containing Egg yolk concentration (10%, 20%) affects viability and acrosome morphology of rabbit sperm. Sperm viability was then assessed for the freezing extenders TCGD (Tris + Citricacid + Glucose + DMSO), TCGED (Tris + Citricacid + Glucose + Egg yolk + DMSO), TCGGD (Tris + Citricacid + Glucose + Glycerol + DMSO) and TCGSD Tris + Citricacid + Glucose + Sucrose + DMSO) during thawing at 38oC. for 20 seconds, respectively. TCG + 10% egg yolk (viability: 77.0 ± 0.8, NAI: 73.3 ± 0.9) was significantly (sperm viability and normal acrosome interaction (NAI)) higher than TCG + 20% egg yolk (70.7 ± 1.1, 70.0 ± 0.9) in the sperm normalcy analysis according to the yolk concentration. TCGGD (53.4 ± 0.1, 62.3 ± 0.4), TCGSD (61.3 ± 0.0, 67.1 ± 0.1) sperm viability and normal acrosome interaction (NAI) in frozen spermatozoa are TCGD (46.4 ± 2.8 and 56.3 ± 1. 4) and TCGED (23.0 ± 1.1 and 54.6 ± 1.4) extenders was thawed at 38oC for 20 seconds. According to the results from each frozen bulking agent, sperm membrane integrity by hypotonic swelling test (HOST) analysis in TCGGD (59.8 ± 0.7), TCGSD (59.3 ± 0.5) was significantly high compared to other experimental groups (p < 0.05). In conclusion, these results suggested that TCGGD and TCGSD extenders enhance survivability of rabbit sperm after frozen-thawing.

테크놀러지 시대의 東道西器論

김상환 서울대학교 철학사상연구소 2003 철학사상 Vol.16 No.spc1-4

Detection of Matrix Metalloproteinases Patterns in Bovine Luteum cell during Pregnancy

김상환,김경래,이지혜,신다혜,정나현,이호준,윤종택 사단법인 한국동물생명공학회 2018 한국동물생명공학회지 Vol.33 No.2

The major focus of this study is to analyze the expression of bovine MMPs and to monitor their activity during the estrus cycle and pregnancy. During pregnancy, MMP-2 expression was detectable around 30 days but became insignificant by 60 days, then started to increase again around 90 days and reached the maximum at 250 days. The activity of MMP-2 protein changed in accordance with its expression level. As expected, the level of TIMP-2 exhibited a reverse pattern. About MMP-9, high level expression was observed as early as 30 days and gradually increase until 90 days. Then started to decrease after 250 days. Again, the sites of MMP-9 expression were similar to those of MMP-2. On the other hand, expression of TIMP-3 remained low until 90 days but showed a small and temporal increase around 250 days. In summary, expression of different MMPs were differentially regulated during estrus cycle and pregnancy. While the expression of MMP-2 was high in estrus cycle, MMP-9 slowly takes over with the progression of pregnancy. These results indicated that the luteal tissue perform distinct functions during pregnancy and estrus. Perhaps the activity of MMP-2 is required for the structural remodeling of luteum, resulting the suppression of P4 inflow from blood. On the other hand, steady maintenance of MMP-9 throughout luteal development is important for the activation of cell proliferation, maturation and angiogenesis.

규제 및 감독 : 은행의 BIS 자본비율 제고방안

김상환 한국금융연구원 2004 KIF 은행경영브리프모음집 Vol.2004 No.-

대단면 터널 라이닝 콘크리트 타설 기법 개선에 관한 연구

김상환,나경웅,고성일,Kim, Sang-Whang,Ra, Kyong-woong,Koh, Sung-Yil 한국터널지하공간학회 2006 터널기술 Vol.8 No.1

터널라이닝에 대한 필요성 여부에 대한 논란은 지속적으로 되고 있으나, 대부분의 터널기술자들은 라이닝의 필요성에 대하여 동의하고 있다. 따라서 설계 및 시공상의 라이닝 특성을 고려한 콘크리트 라이닝 품질향상방안 수립을 위하여 근본적인 문제점을 파악하고 해결하는데 도움이 되고자한다. 본 논문에서는 균열발생량이 상대적으로 많은 대단면 4차로 터널을 대상으로 하여 콘크리트라이닝 시공에서부터 터널운용전 발생되는 초기균열에 대한 발생원인 분석을 실시하고, 국내 콘크리트 라이닝 시공특성을 파악하기 위한 사례조사를 실시하였다. 이를 바탕으로 하여 균열발생 최소화를 위한 시공개선방안을 제안하고, 개선방안에 대한 라이닝 콘크리트 타설모형실험 및 강재거푸집내 콘크리트의 유동분석을 수행하여 고품질 콘크리트 라이닝시공을 위한 제안방안의 효율성을 검증하였다. Although the adverse critisism have been continued that the concrete lining need or not, most tunnel engineer agree that the concrete lining needs to increase the tunnel safety To establish the concrete lining's quality improvement method, we grasp the basic problems in lining construction process and propose the lining construction methods to increase the concrete quality This paper present a reason of initial crack occurrence in the large section tunnel (4 lane wide span tunnel), lining and an improved method for crack minimization in construction process. It is also verified verify that the proposed method can give qualified concrete lining by carrying out the concrete injection model test and the numerical analysis of concrete flow.

동결융해 후 토끼 정자의 Matrix Metalloproteinases (MMPs)와 Their Tissue Inhibitors (TIMPs) 발현 양상

김상환,최화식,윤종택 한국동물생명공학회(구 한국동물번식학회) 2019 Journal of Animal Reproduction and Biotechnology Vol.34 No.3

We observed MMPs expression in all sperm groups, with pro-MMP showing lower expression than active MMPs. According to the results from each freezing extender, the sperm membrane integrity (HOST: Hypoosmotic Swelling Test) analysis in TCGGD (Tris 250 mM, Citric acid 88 mM, Glucose 47 mM, Glycerol 3%, Dimethylsulpoxide 3.5 M) is 59.8 ± 0.7, TCGSD (Tris 250 mM, Citric acid 88 mM, Glucose 47 mM, Sucrose 0.1 M, Dimethylsulpoxide 3.5 M) is 59.3 ± 0.5 were significantly higher (p < 0.05) among the experimental groups. And MMPs analysis result, we observed MMPs expression in all sperm groups, with pro-MMP showing lower expression than active MMPs. The expression of active MMP-2 was the highest in sperms frozen in TCGSD and TCGD (Tris 250 mM, Citric acid 88 mM, Glucose 47 mM, Dimethylsulpoxide 3.5 M), Meanwhile, sperms from the TCGGD and TCGED (Tris 250 mM, Citric acid 88 mM, Glucose 47 mM, Ethylene glycol 3%, Dimethylsulpoxide 3.5 M) group showed lower level of active MMP-2 expression. Together, these results indicate that adding glycerol or sucrose to the sperm freezing buffer would not only suppress MMPs expression but also minimize DNA fragmentation, providing a mean to improve the success rate in the in vitro manipulation of rabbit sperms. Therefore, these results suggest that TCGGD or TCGSD extender method for freezing-thawing of rabbit sperm increased the viability after thawing.

무지외반증에서 생체흡수성 나사못으로 고정한 변형 마우 절골술의 결과

김상환,윤영필 대한족부족관절학회 2015 대한족부족관절학회지 Vol.19 No.3

Purpose: The purpose of this study was to analyze the clinical results of application of bioabsorbable screws in hallux valgus surgery using modified Mau osteotomy. Materials and Methods: We retrospectively reviewed medical records of 25 patients. Operations were performed between May 2013 and January 2014. We performed 33 modified Mau osteotomies and fixed using bioabsorbable screws. Mean age of patients was 52 years (range 19 to 71). Mean follow up duration was 13.2 months (range 12.3 to 18.9). The clinical evaluations included pain visual analogue scale (VAS) score, American Orthopaedic Foot and Ankle Society (AOFAS) score, and satisfaction score. Weight bearing anteroposterior radiographs were taken for measurement of hallux valgus angle (HVA), intermetatarsal angle (IMA), and distal metatarsal articular angle (DMAA). All radiographs were evaluated in order to detect complications related to bioabsorbable screws such as osteolysis, cyst formation, and fixation failure. Results: The mean pre- and postoperative pain VAS scores were 4.0 and 1.7 (p<0.05). The mean AOFAS score improved from 52.6 to 82.8 (p<0.05). Preoperative HVA and IMA were 31.2 and 13.9, respectively. Postoperative HVA and IMA were 5.2 and 6.2 (p<0.05). The DMAA increased from 7.8 to 9.9 (p<0.05). There was one case of superficial wound infection and one loss of correction, and no case of osteolysis, cystic formation around the screw, or deep infection. All patients showed union without fixation failure. Conclusion: The clinical and radiological evaluation of this study demonstrates reliable results without fixation failure or allergic reaction. The use of bioabsorbable screw appears not to be inferior to metal screw fixation in hallux valgus surgery.

책의 존재와 사물의 존재(6)-글쓰기와 미메시스

김상환,Kim, Sang-Hwan 대한출판문화협회 1994 출판저널 Vol.154 No.-

소크라테스는 진리를 언어 밖에서 찾으라고 말했다. 플라톤은 언어를 어떤 모방의 형식이라고 생각했다. 미메시스 개념에 기초한 언어관 때문이다. 따라서 그들에 의하면, 책이란 진리의 시체 통조림이자 영혼을 망쳐놓는 흉물에 불과하다. 그러나 아리스토텔레스의 "오르가논"은 언어를 상징의 형식으로 이해하면서 책을 진리의 잔치마당으로 여긴다.

Influence of Autophagy Induction after Hormone Treatment on Oocytes Maturation of Porcine

김상환,윤종택 사단법인 한국동물생명공학회 2018 한국동물생명공학회지 Vol.33 No.4

Here, we evaluated the mode of programmed cell death during porcine oocyte maturation by comparing the two major pathways associated with programmed cell death, apoptosis (type I), and autophagy (type II). We investigated the expression and localization of major genes involved in autophagy and apoptosis at mRNA and protein levels. Furthermore, the effect of hormonal stimulation on autophagy and apoptosis was analyzed. We found that the activity of autophagy-associated genes was increased in the cumulus-oocyte complexes (COCs) following follicle-stimulating hormone (FSH) treatment, while the addition of luteinizing hormone (LH) reversed this effect. The expression of proteins associated with autophagy was the highest in FSH-treated COCs. On the other hand, caspase-3 protein level was maximum in COCs cultured with LH. The treatment with rapamycin resulted in the effect similar to that observed with FSH treatment and increased autophagy activity. Thus, hormonal stimulation of pig oocytes resulted in distinct patterns of maturation. The high-quality oocytes majorly relied on the type II pathway (autophagy), while the type I pathway (apoptosis) was more prominent among poor-quality oocytes. Further investigation of this distinction may allow the development of techniques to produce high-quality oocytes in porcine in vitro fertilization