안와골절 수술 후 발생한 두 가지 상피세포를 가진 안와 점액낭종 1예

김미진,이민정,김찬중,김남주,정호경,곽상인,Mijin Kim,MD,Min Joung Lee,MD,Chan-Joong Kim,MD,Nam Ju Kim,MD,Ho-Kyung Choung,MD,Sang In Khwarg,MD 대한안과학회 2010 대한안과학회지 Vol.51 No.7

Purpose: The authors report a case of orbital mucocele lined with two types of histological epithelial cells developed after repair of the orbital wall fractures. Case summary: A 53-year-old man presented with proptosis of the left eye for two years. The patient had a history of left orbital inferomedial wall fracture repair ten years earlier at a different hospital. Examination revealed 4 mm proptosis and superior globe displacement of the left eye. Restriction of left ocular movements on elevation, depression and adduction were observed. A computed tomography scan demonstrated a large, non-enhancing, cystic tumor in the left inferior orbit with the inferior and medial wall displaced toward the paranasal sinus. An orbital cystic tumor was excised with the removal of previously-inserted orbital implant via a transconjunctival and transcaruncular approach. The inferior, and medial orbital walls were reconstructed using a MEDPOR® TITANTM implant. The initial pathologic diagnosis was epidermal cyst. Histopathologic re-review revealed an orbital cyst lined with both stratified squamous epithelium and pseudostratified ciliated columnar epithelium, thus diagnosis was changed to orbital mucocele. Proptosis and restriction in ocular motility improved postoperatively. Conclusions: Mucocele formation should be considered in patients in whom a cyst developed after orbital fracture repair.

In vitro Screening of Jeju Island Plants for Cosmetic Ingredients

김미진,정택규,김무한,윤경섭,Kim, Mi Jin,Jung, Taek Kyu,Kim, Moo-Han,Yoon, Kyung-Sup Korean Society for Biotechnology and Bioengineerin 2018 KSBB Journal Vol.33 No.2

To identify new active cosmetic ingredients of natural origin, we screened the biological activities of 50 different kinds of plants from Jeju Island, the southernmost island of the Korean Peninsula. In this study, plant extracts were screened for inhibitory effects on elastase, nitric oxide, and melanin production, and for free radical scavenging effects. Eight plants, Viburnum erosum Thunb., Trachelospermum asiaticum var. intermedium Nakai, Sageretia theezans Brongn., Sorbus alnifolia var. hirtella T. Lee, Staphylea bumalda DC., Pourthicea villosa var. brunnea Nak., Illicium religiosum S. et Z., and Castanopsis cuspidata var. sieboldii Nakai were potent inhibitors of elastase (over 80% inhibition at 1 mg/mL). Elaeocarpus sylvestris var. Ellipticus (Thunb.) Hara ($IC_{50}$: $13.99{\mu}g/mL$), Sapium sebiferum (L.) ROXB. ($IC_{50}$: $14.27{\mu}g/mL$), and Castanopsis cuspidata var. sieboldii Nakai ($IC_{50}$: $16.46{\mu}g/mL$) showed good antioxidant effects. Eurya japonica Thunb., Quercus myrsinaefolia Bl., Caesalpinia decapetala var. japonica (S. et Z.) Ohashi, Weigela subsessilis L. H. Bailey, and Crinum asiaticum var. japonicum dose-dependently inhibited LPS-induced NO production on RAW 264.7 cells (at $50-200{\mu}g/mL$). Furthermore, Elaeocarpus sylvestris var. Ellipticus (Thunb.) Hara, Ilex integra Thunb., and Agastache rugosa (Fisch. et Meyer) O. Kuntze dose-dependently inhibited ${\alpha}-MSH$ stimulated melanin synthesis on B16 F10 cells (at $25-100{\mu}g/mL$). These results suggest that Jeju Island plants possess several biological activities and may be potent anti-aging, anti-inflammatory, skin-whitening, and anti-oxidative ingredients as functional cosmetic materials.

폐동맥 분지협착에 스텐트 삽입 후 유발되는 폐동맥의 형태변화 - 폐동맥 폐쇄 부전의 역할 -

김미진,강두철,최재영,이종균,설준희,이승규,Kim, Me Jin,Kang, Du Cheol,Choi, Jae Young,Lee, Jong Kyun,Sul, Jun Hee,Lee, Sung Kyu 대한소아청소년과학회 2003 Clinical and Experimental Pediatrics (CEP) Vol.46 No.1

목 적 : 분지 폐동맥 협착 부위에 스텐트를 삽입하여 효과적으로 교정한 후, 이후 추적 관찰시에 협착이 없었던 반대편 분지 폐동맥 단면적이 감소하지 않고 증가하는 양상을 관찰하였다. 이에 분지 폐동맥 단면적의 변화에 영향을 끼치는 형태학적 혈역학적 요인들을 분석해 보았다. 방 법 : 1995년 1월부터 2002년 7월까지 연세대학교 심장혈관병원 소아심장과에서 좌폐동맥 분지 협착을 진단받고 스텐트 삽입을 시행받은 23명의 환아를 대상으로 하였다. 이들 환아에서 스텐트 삽입 전후로 심혈관 조영술을 통해 좌우 폐동맥 단면적의 변화, 도플러 심초음파 검사를 시행하여 폐동맥 부전을 측정하여 역류 분율의 변화를 관찰하였고, 폐관류 검사를 함께 시행하였다. 결 과 : 1) 좌폐동맥 협착으로 스텐트를 삽입한 후 좌폐동맥 지수는 $102{\pm}12mm^2/BSA$에서 $125{\pm}11mm^2/BSA$로 유의하게 증가하였다(P=0.001). 2) 협착이 없었던 우폐동맥 지수의 변화를 보면, 좌폐동맥에 스텐트를 삽입하기 전에 평균 $238{\pm}17mm^2/BSA$에서 추적 검사시 $249{\pm}20mm^2/BSA$로 유의한 증가는 없었다(P=0.474). 3) 스텐트 삽입 전후로 양측 폐동맥 지수의 합을 보면, 삽입전 $340{\pm}21mm^2/BSA$에서 추적 검사시 $374{\pm}26mm^2/BSA$로 증가하는 경향이 있었지만 유의한 차이는 없었다(P=0.09). 4) 폐동맥 폐쇄부전 역류 분율의 변화는 스텐트 삽입전 $50{\pm}5%$에서 $46{\pm}5%$로 감소하였으나, 유의한 감소량은 아니었다. 그러나, 폐동맥 폐쇄부전 역류 분율 증감 정도에 따라 세 군으로 나누어 비교해 보면, 3 군에서 우폐동맥지수는 삽입 전 $260{\pm}23mm^2/BSA$에서 추적 검사시 $325{\pm}33mm^2/BSA$으로 유의하게 증가하였다(P=0.041). 좌폐동맥 지수는 각 군에서 스텐트 삽입 전후로 증가하는 양상은 관찰되었으나, 1군에서만 유의하였고, 이는 폐동맥 폐쇄부전의 역류 분율 증감이 좌폐동맥의 단면적에 변화를 미치는 일차적 요인은 아닌 것으로 분석된다. 5) 스텐트 삽입 후 좌 우폐동맥 모두 폐동맥 폐쇄부전이 심할수록 폐동맥지수가 증가하여 유의한 양의 상관 관계를 가지고 (좌폐동맥; r=0.69, P<0.01 및 우폐동맥; r=0.53, P<0.01), 회귀분석상으로도 유의한 관계를 보였다. 또한 양측폐동맥 지수의 합도 유의한 양의 상관 관계(r=0.71, P<0.01)를 보였다. 결 론 : 협착이 있던 좌폐동맥은 스텐트의 삽입으로 교정되어 그 단면적 지수가 증가하였다. 그리고, 반대편 우폐동맥의 경우에는 잔존하는 폐동맥 폐쇄부전의 영향으로 단면적 지수가 감소하지 않고, 오히려 증가한 것을 관찰하였다. 그러나, 잔존하는 폐동맥 폐쇄부전의 장기적 예후를 고려해 볼 때, 이를 예방할 수 있는 것이 중요하다. 또한 역류 분율을 정확히 산출하기 위해 자기공명영상을 이용하는 것도 적극 검토해야 할 것이다. Purpose : Intravascular stent implantation for the treatment of postoperative branch pulmonary artery(PA) stenosis has been used successfully. However, the cross sectional area of contralateral branch PA does not regress in spite of the successful dilation of the stenotic branch PA after stent implantation. We analyzed the morphologic and hemodynamic factors on the size of branch PA after successful stent implantation. Methods : The subjects in our study were 23 children who had undergone stent implantaion from Jan. 1995 to Jul. 2002 in the Division of Yonsei Pediatric Cardiology. We evaluated the cross sectional area index(CSAI) of branch PA before and after stent implantation at follow-up catheterization. We also investigated factors such as residual pulmonary stenosis, pulmonary regurgitation(PR), systolic pressure of right ventricle, and lung perfusion scan. Results : The CSAI of the RPA without stenosis changed from mean $238{\pm}17mm^2/BSA$ to mean $249{\pm}20mm^2/BSA$(P=0.47), but didn't regress. The CSAI of the LPA with stenosis was increased effectively by stent implantation from the mean $102{\pm}12mm^2/BSA$ to mean $125{\pm}11mm^2/BSA$(P< 0.05). At follow up after stent implantation, the CSAI of PA is correlated with the residual PR fraction after stent implantation. Conclusion : In a group with increased residual PR, CSAI of RPA was found to be significantly increased between the pre- and post-stages of stent implantation. So, we suspect that the pulmonary regurgitation remaining after right ventricle outlet tract(RVOT) dilatation surgery is correlated with the increase in CSAI of RPA.

고등학생의 자아존중감이 장애인에 대한 낙인에 미치는 영향

김미진,Kim, Mi Jin 한국농촌간호학회 2015 한국농촌간호학회지 Vol.10 No.2

Purpose: This study was done to investigate the effect of self-esteem on perceived stigma in high school students toward people with disabilities. Methods: The participants in this study were 140 high school students from Gyeonggi Province. Data were collected during May 2013. Structured questionnaires were used for data collection. Data were analyzed using descriptive by statistics, t-test, ANOVA, Pearson correlation coefficients, and simple regression analysis with the SPSS 19.0 program. Results: The major findings of this study are as follows: There was a significant relationship between stigma toward people with disabilities and self-esteem among the high school students (r=-.205, p=.015). There was also a significant relationship between relationships with friends (r=.291, p<.001), health statement (r=.400, p<.001) and self-esteem among the high school students. Self-esteem explained 3.5% of the variance in high school students' stigma toward people with disabilities (F=6.041, p=.015). Conclusion: Findings of this study suggest a need to develop education to increase self-esteem as a strategy to decrease high school students' stigma toward people with disabilities. Also, findings of this study suggest the need for repeated study on the relationship between self-esteem and stigma toward people with disabilities among adults.

IP 네트워크에서 IPTV 방송 화질에 관한 연구

김미진,장종욱,Kim, Mi-Jin,Jang, Jong-Wook 한국정보통신학회 2009 한국정보통신학회논문지 Vol.13 No.7

최근 멀티미디어 콘텐츠가 빠르게 확산되고, 초고속 광대역 IP 네트워크 기술이 발전하면서 통신과 방송의 독자적 개별 서비스가 서로 공존하여 통합융합 서비스로 진화발전하고 있다. 대표적 방송/통신 융합 서비스인 IPTV는 통합융합서비스의 가장 강력한 응용분야이며, IPTV와 같은 서비스들이 증가함으로 인해 다양한 인터넷 서비스를 구현하기 위해서 인터넷 프로토콜을 통한 고화질의 데이터 처리에 대한관심이 통신업계의 화두로 등장하고 있다. 본 연구에서는 소규모에서 사용되는 표준 라우팅 프로토콜인, RIP 환경과 대규모 자율 네트워크에서 사용되는 링크 상태 라우팅 프로토콜인, OSPF 환경을 구현하여 IPTV 서비스 중에서 방송 화질의 PSNR값으로 성능을 측정하고 평가하여 효율적인 IPTV 서비스를 위한 네트워크 구축 방안을 제시하고자 한다. The multimedia contents are diffused rapidly. Also, the course individual service of communication and broadcasting coexists with each other and develop to the integrated fusion service with development of the high speed broadband IP network technology. The IPTV which is representative broadcasting a communication total service is the powerful application field. The communication industry has been interested in data processing of the high-definition that supports many kinds of internet service by internet protocol. In this paper, we configure the standard routing protocol use in the small scaled, on RIP environment and the link status routing protocol use the large scale autonomous network, on OSPF environment. We also measure and evaluate broadcasting quality by PSNR(Peak Signal-to-Noise Ratio). Finally, we propose the network construction plan for IPTV services which are efficient.

Exposure to mercury induced early apoptotic signals in human placental BeWo cells through alteration of cell cycle regulation

김미진,김철홍,An Mi-Jin,Shin Geun-Seup,Lee Hyun-Min,김지영,Hwang Jae Yoon,이주현,Kim Jung-Woong 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.4

Backgrounds Heavy metals are environmental pollutants and their breakdown is regarded as a serious risk to human health. Despite increasing evidence that heavy metals have adverse efects in vivo and in vitro, there is no evidence of the efect of heavy metals during placental formation. Objective We determined the efect of heavy metals on cell viability of BeWo human placental cells using MTS assay and live and dead assay. We also evaluated cell proliferation, cell cycle, and apoptosis by heavy metal treatment using FACS analysis. Results Mercury chloride induces severe cell cycle arrest at the sub-G1 phase by the accumulation of cyclin B. Furthermore, we identifed that mercury chloride induces apoptosis by enhancing the activity of caspase-3. However, we were unable to confrm the deleterious efect of lead in BeWo cells. Conclusion Our results suggested that exposure to heavy metals, specifcally mercury chloride, induced cytotoxic efects in BeWo cells through cell cycle arrest and apoptosis.

생명과학 분야 연구자들의 연구 성과 분석 연구 - K 대학교를 중심으로 -

김미진,Kim, Mee-Jean 한국도서관정보학회 2020 한국도서관정보학회지 Vol.51 No.2

본 연구의 목적은 정부로부터 많은 연구개발비의 지원과 더불어 연구역량 및 국제적 연구네트워크를 갖추고 있는 K대학교 생명과학과 교수진이 발표한 14년간(2004년-2017년)의 학술지 논문을 대상으로 연구개발비 재원별, 주제 영역별, 발표 주요 학술지, 연구 유형별로 조사함으로써 국내 생명과학 분야 선도 연구자들의 연구 발행 패턴의 특성을 파악하였다. 또한 교수진이 발표한 논문의 발행 패턴에 따른 15년간(2004년-2018년) 즉시 피인용 빈도 및 피인용 빈도, 주제 영역 학술지 영향계수(IF) 4분위 수 등 피인용도와의 관계를 분석하였다. 연구개발비 지원 유형에 따른 논문 1편당 즉시 피인용 빈도를 Welch 검증 분석 결과, 세 가지 유형 중 해외기관 재원에 의한 연구 논문이 1.8회로 가장 높았으며 통계적으로 유의미한 차이가 있는 것으로 나타났다(F = 10.467, p < .000***). 또한 공동연구 유형별로 논문 1편당 즉시 피인용 빈도 및 논문 1편당 피인용 빈도에서 해외 기관과의 국제 공동연구 논문이 다른 세 가지 공동연구 유형에 의한 논문과 비교해 더 많이 인용된 것으로 분석되었다. The purpose of this study is to provide an in-depth analysis on the publication patterns of the 1,029 journal articles by the Life Sciences' faculty at the K University, for the years 2004-2017 and to investigate any difference between their publication patterns and the citedness for the years 2004-2018. Among the three funding agency types, the research publications supported by foreign funding received the more citations than publications by other types of funding, and the study also found a statistical difference in the citedness (F = 10.467, p < .000***). In addition, the internationally co-authored publications received more citations than three other types of co-authored publications in terms of the immediacy index per publication and the average citations per publication.

수확 후 제주 당근 재배 토양에서 Xanthomonas hortorum pv. carotae 분리

김미진,강현수,신용호,전용철,Mi-Jin Kim,Hyun Su Kang,Yong Ho Shin,Yong Chull Jeun 한국식물병리학회 2023 식물병연구 Vol.29 No.4

당근 세균잎마름병은 세계적으로 가장 중요한 병에 속한다. 지난 10년 전 국내에서 처음 발견됨으로써 당근 생산에 큰 위협이 되는 병으로 간주되었다. 원인균으로 Xathomonas hortorum pv. carotae (Xhc)이며 본 연구는 제주도 당근재배지 토양에서 이 병원균을 분리 동정하고자 수행하였다. 분리한 세균 중 인공배지에서 균총의 형태가 당근 세균잎마름병균과 유사한 세균균총을 분리하여 선발하였다. 또한 이들 선발된 세균들을 대상으로 DNA 염기 서열과 NCBI 자료를 통해 몇몇 분리 세균이 Xhc으로 동정되었다. 게다가 이들 세균을 당근 잎에 접종하였더니 당근 세균잎마름병의 전형적인 병징이 나타났다. 본 연구 결과는 제주도 토양내 Xhc의 존재 가능성을 시사하고 앞으로의 당근 세균잎마름병 방제 수단을 강구하는 데 매우 필요한 자료로서 가치가 있다고 본다. Bacterial leaf blight in carrot is one of the most important diseases in the worldwide. In the past decade, its introduction into Korea is causing great concern due to the potential damage to carrot crops domestically. This bacterial disease is caused by Xanthomonas hortorum pv. carotae (Xhc). This study aimed to isolate and identify bacterial strains from the soil of carrot farms in Jeju Island. The bacterial isolates showing characteristics similar with those of Xhc were selected when cultured on artificial media. Through DNA sequencing and analysis based on NCBI data, some of the selected bacterial strains were identified as Xhc. Furthermore, the bacterial strains caused the typical symptom of bacterial leaf blight after inoculation on carrot leaves. The results of this study showed the potential establishment of Xhc in the soil of Jeju Island and it may be valuable data for establish a strategy preventing the domestic spread of carrot bacterial leaf blight in the future.

Comparison of the cytotoxicity of bisphenol A and its analogs on human placental BeWo cells

김미진,Jo Ah-Ra,김지영,An Mi-Jin,Shin Geun-Seup,Lee Hyun-Min,Kim Jinho,박진홍,김철홍,Kim Jung-Woong 대한독성 유전단백체 학회 2023 Molecular & cellular toxicology Vol.19 No.3

Background Bisphenol A (BPA) and its alternatives (BPF, BPS) are widely used in daily consumer products, which commonly expose humans to these chemicals. Bisphenols (BPs) are endocrine-disrupting chemicals widely present in the environment and can accumulate in the entire food chain by circulating in the ecosystem. In humans, BPs intake leads to accumulation in the human body and causes many chronic diseases, including endocrine disruption, immunotoxicity, and cancer. Objective Although several studies have demonstrated the negative eff ect of BPA and its alternatives, studies have yet to be determined whether bisphenols can aff ect human trophoblast cells. Thus, this study aimed to analyze the toxic eff ect of BPs on the human trophoblast cell line BeWo. Results We investigated the cytotoxic eff ect of BPs on human trophoblast cells. The cytotoxicity was measured by the MTS assay and fl ow cytometric analysis that representing the cell viability, cell proliferation, cell cycle progression and apoptotic cell death. BPs had no eff ect on BeWo cell viability at concentrations below 100 mM, and did not cause signifi cant changes in cell proliferation and apoptosis. However, BPA and BPS altered cell cycle progression and checkpoint regulator expression. Conclusion BPs did not aff ect cell viability and proliferation. However, BPS altered the G 0 /G 1 and G 2 /M phases of the trophoblast BeWo cells by increasing cyclin B1 expression.

망막에서 기인한 기형성 수질상피종 1예

김미진,정호경,이민정,김영아,곽상인,Mijin Kim,Ho-Kyung Choung,Min Joung Lee,Young A Kim,Sang In Khwarg 대한안과학회 2010 대한안과학회지 Vol.51 No.12

Purpose: To report a rare case of medulloepithelioma originating from the retina. Case summary: A nine-year-old boy visited our clinic with conjunctival injection and decreased visual acuity in the left eye, which had developed two month previously. Slit lamp examination revealed a distorted pupil and an anteriorly protruding ciliary body displacing the peripheral iris toward the cornea on the inferonasal side. Funduscopic examination showed total retinal detachment accompanied by an inferior hypervascular mass. The examination of the right eye was unremarkable. On computer tomography imaging, a calcified mass was identified behind the iris of the left globe in the inferomedial aspect, and ultrasound biomicroscopy revealed a medium to high echogenic tumor with an uneven oval cystic cavity in the ciliary body. At the follow-up examination, the size of the mass was increased, so we performed enucleation of the left eye. Pathology demonstrated that the retrolental mass abutting the lens had arisen from the retina. Histological examination revealed that the tumor had originated from the retina and extended into the ciliary body, and most of the tumor was composed of hyaline cartilage with calicification. Tumor cells were identified in the periphery, forming elongated tubules and cord-like structures that were immunohistochemically positive for vimentin, neuron-specific enolase, and CD56 compatible with a teratoid medulloepithelioma. The patient was followed up for eight months without any metastasis in the orbit or elsewhere. Conclusions: Medulloepithelioma should be considered in the differential diagnosis of pediatric orbital mass accompanied by calcification. J Korean Ophthalmol Soc 2010;51(12):1665-1670