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β-galactosidase 생산 우수 유산균 선별 및 배지 최적화
김명국(Myung-Kuk Kim) 한국산업기술융합학회(구. 산업기술교육훈련학회) 2022 산업기술연구논문지 (JITR) Vol.27 No.4
김치, 요구르트, 지렁이 분변토로부터 MRS 고체 배지와 X-gal 에 있어서 활성을 나타낸 7개의 균주를 선별하였으며 이들 중 요구르트에서 분리해낸 균주가 가장 우수한 균주로 선정하였다. β-galactosidase 활성 측정값은 균체나배양 여액보다는 균체 현탁액을 이용했을 때가 가장 높게 나타났다. 분리균은 그람 염색 및 일부 생화학적 실험결과와시판중인 요구르트로부터 분리되었다는 결과를 통해 Lactobacillus속 유산균으로 유추하였으며 균체 생육과 효소생산을 위한 탄소원, 질소원, 무기염류의 최적농도를 조사하였다. 이상의 결과는 안전성이 담보된 유산균을 이용한 β -galactosidase 생산 연구에 적용될 수 있을것으로 사료된다. Seven strains showing activity in MRS(De Man, Rogosa, and Sharpe) solid medium containing X-gal were selected from kimchi, yogurt, and earthworm feces. The strain isolated from yogurt showed the greatest activity. Enzyme activity was higher in cell suspensions than in cells or culture filtrates. The isolate was inferred to be a lactic acid bacterium based on Gram staining, biochemical tests, and the fact that it was isolated from commercial yogurt. The optimal concentrations of carbon, nitrogen, and minerals for cell growth and enzyme production were investigated. The results of this study are applicable to the production of β-galactosidase using lactic acid bacteria.
DK1002에 대한 급성독성시험 및 유전독성에 관한 연구
류재천,김경란,김현주,정상운,김명국,박희석,김용해,Ryu, Jae-Chun,Kim, Kyung-Ran,Kim, Hyun-Joo,Jung, Sang-Oun,Kim, Myung-Kuk,Park, Hee-Sock,Kim, Yong-Hae 한국독성학회 1998 Toxicological Research Vol.14 No.3
The acute and genetic toxicity of DK1002 was subjected in this study. DK1002 which is a morphine-like new drug candidate synthesized by Dong-Kook Pharmaceutical Co. Ltd. is now under developing as a analgesics that have better drug efficacy and least addictive property. In acute toxicity study, the 50% lethal doses ($LD_{50}$) of DK1002 were determined as>2000mg/kg (p.o.), 237.0mg/kg(i.p.), 57.5mg/kg(i.v.), and 1266.9mg/kg (s.c.). And also, to study the genotoxicity of DK1002, we performed bacterial reversion assay with Salmonella typhimurium TA98, TA100, TA1535, and TA1537, and in vitro chromosomal aberration assay with Chinese hamster lung cells in the presence and absence of S-9 metabolic activation system. In vivo micronucleus assay using mouse bone marrow cells was also performed. From these results, DK1002 was revealed nonmutagenic potential in S. typhimurium TA98, TA100, TA1535, and TA537 both in the absence and presecne of metablic activation system. No clastogenicity of DK1002 was observed in chromosomal aberration assay in vitro as well as in micronucleus assay in vivo.