High pressure liquid chromatography에 의한 모유의 유당 함량 분석

김두희,정태호,정원호 慶北大學校 醫科大學 1983 慶北醫大誌 Vol.24 No.2

굴절율 측정기가 부착된 high pressure liquid chromatography(HPLC)를 이용하여 모유내 탄수화물을 분석하는 방법을 검토하고 실제 이 방법을 이용하여 모유의 수유시기별 유당 함량을 정량하였다. 모유를 Waters사 제품의 sep-pak-C_18 cartridge에 통과시켜 단백질과 지질을 제거한 후 탄수화물만 함유된 분획을 얻어 유당 함량을 분석하였다. 첨가 시험시의 유당 회수율은 평균 99.2%였다. 이 방법을 이용하여 모유의 유당 함량을 측정한 결과 초유의 유당 함량은 5.34g/100㎖였으며 성숙유는 6.66g/100㎖로 성숙유가 초유에 비해 높은 치를 나타내었다. 정밀분석 기기의 하나인 HPLC를 이용한 탄수화물의 분석 방법은 앞으로 모유 뿐만 아니라 각종 우유 제품 및 체액에서의 당분석에 응용할 수 있을 것으로 생각된다. A rapid liquid chromatographic procedure for the determination of lactose in the breast milk was described. After removal of proteins and lipids by SEP-PAK C_18 cartidge, the carbohydrate containing fraction was injected into liquid chromatograph. The carbohydrate components were separated with micro-Bondapak carbohydrate analysis column and isocratic elution with an acetonitrile/water mobile phase. The eluent composition was measured with refractive index detector. Lactose and retention time of 8.03 minute. Mean analytical recovery of added lactose was 99.2%. The amount of lactose determination may be applicable to any other carbohydrate determination in the body fluid and milk products.

투명 유연 AMOLED TV 구현을 위한 증착형 SnO₂/Ag-Pd-Cu(APC)/SnO₂ 다층 투명 캐소드 박막 연구

김두희,김한기 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.2

大邱市路上野糞(人糞 및 犬糞)의 人體腸內 寄生蟲卵 檢査成績 : 抄錄

金斗熙 최신의학사 1962 最新醫學 Vol.5 No.3

The comparison of Ag nanowire, Ag film, and Ag-Pd-Cu films inserted InZnTaO multilayer electrode for flexible organic solar cells

김두희,서기원,김효중,이혜민,조다영,김석순,김한기 한국진공학회 2015 한국진공학회 학술발표회초록집 Vol.2015 No.2

잠사회사(蠶絲會社) 여공(女工)의 피부질환(皮膚疾患)

김두희,Kim, Doo-Hie 대한예방의학회 1976 예방의학회지 Vol.9 No.1

잠사회사(蠶絲會社) 근로자(勤勞者)의 직업성질환(職業性疾患)을 분석해 보기 위하여 1975년(年) 11월(月) 근로조건(勤勞條件)이 같은 대구시내(大邱市內) 모잠사회사(某蠶絲會社) 여자근로자(女子勤勞者) 177명(名)에 대하여 집단건강진단시(集團健康診斷時) 시진(視診)에 의하여 실시(實施)한 결과 다음과 같이 요약할 수 있었다. 전(全) 여자근로자(女子勤勞者)에 있여서 각종피부질환(各種皮膚疾患)의 유병률(有病率)이 53%로 반수(半數)이상에 달하여 피부질환(皮膚疾患)은 대체(大體)로 3가지로 대분(大分), 손톱마모(磨耗), 피부(皮膚)의 각화, 나류(裸瘤), 구열(龜裂) 등과 같은 피부변화(皮膚變化)(47.5%)가 가장 많으며 알레르기 반응(反應)(14.7%)과 기타 무좀 등의 순이었다. 피부변화(皮膚變化)는 그 진행정도(進行程度)의 차이(差異)가 있었으나 대개 복합적(複合的)으로 발생(發生)하여 근속년수와는 무관한 것 같으나 대체적으로 2년이상 근속자에게 빈발하는 경향을 보이며 손톱마모(磨耗)는 이에 따라 현저(顯著)한 차이(差異)를 보이고 있다. 알래르기 반응(反應)은 입사후(入社後) 수개월 이내에 발생(發生)하며 다시 접촉 여하에 따라 그 정도로 달라지게 되는 것 같다. 계속 근무자보다 과거에 경험한 바 있는 자에서 다소 그 빈도가 높은 경향을 보여 준다. 호발부위(好發部位)는 역시 접촉이 잦은 손가락부터 손바닥, 손, 팔, 다리, 얼굴, 목, 몸통등 광범하게 번져가며 심한 경우 습진 양으로 변해 가고 있다. The occupational skin diseases in an industry of silk reeling located in Daegu city of Korea were analyzed by inspection through a mass survey for improving the workers' health, November, 1975. The subjects, 177 persons were all females, and were distributed at 18-38 years old. About two thirds were under 25 years old. The meanage was 23.6 years. The incidence of skin diseases was about 53%. It was generally divided into three groups;- the epidermic changes of those were most frequent, 47.5%, the allergic reactions were 14.7%, and athlete's foot was only four cases, 2.3%. Most frequent signs of epidermic changes was the worn-out nail, 32.3%; and the keratolysis, 28.8%; and the callus, 15.3% were followed in order. Theses were significant in five per cent level between the sections of the silk reeling and the manufacturing;-Allergic reactions and worn-out nail were more frequent in the manufacturing group than the silk reeling, and keratolysis and callus were inverted proportionally. Allergic reactions were occurred within a few months from first entrance time, and it was more frequent in the group exposed to same allergen in the past than the continuing group from first. Most of the appearing sites of theses were hands, and infrequently, it was occurred in the face, neck, trunk, knee anfeet. These epidermic changes except allergic phenomena were appeared in the fingers, palmal and dorsal surface of hands frequently contacted. But these changes and allergic reactions were generally combined with two or more kinds.

도금공(鍍金工)에 대한 임상적(臨床的) 관찰(觀察)

김두희,Kim, Doo-Hie 대한예방의학회 1977 예방의학회지 Vol.10 No.1

${\ulcorner}$크롬${\lrcorner}$과 ${\ulcorner}$니켈${\lrcorner}$등의 도금(鍍金)을 하는 도금공(鍍金工)에 대하여 그 직업성질환(職業性疾患)을 관찰(觀察)하고 저 1976년(年) 6(월)月 대구시내(大邱市內) 소재(所在)하는 서로 유사한 종류와 방법으로 작업하고 있는 중소기업체(中小企業體) 중 작업환경개선(作業環境改善)이 어느 정도 되어 있는 곳과 전연 되어있지 않는 곳을 각각 1개소(個所)씩 선택(選擇)하며 비교해 본 결과 다음과 같이 요약(要約)할 수 있었다. 전반적(全般的)으로 비강내(鼻腔內) 증상(症狀)이 총출현빈도(總出現頻度) 중 50%이상을 점하며 다음 현기증(眩氣症)이 문제(文題)로 되어 있으며 환경개선(環境改善)을 함에 따라 비강내증세(鼻腔內症勢)중에서 괴양성 병변(病變)을 줄이어 증상(症狀)은 가볍게 되었으나 개입보호구사용(個入保護具使用)을 소홀히 함으로써 점차 만성적인 변화(變化)를 초래할 염려가 있었다. 각종 혈액상(血液相)중 ${\ulcorner}$헤모글로번${\lrcorner}$, ${\ulcorner}$헤마토크리트${\lrcorner}$ 적혈구(赤血球)와 요소견(尿所見)중 단백질당(蛋白質糖), ${\ulcorner}$우로비리노겐${\lrcorner}$등에는 큰 영향을 미치지 않고 있다고 생각된다. This is a result of clinical examination for workers working with Chromic acid and Chromium compounds in the plating room of their companies. I selected two companies that the plating process and the kinds of plating were similar. One (SW Co.) was more or less improved the operating environment with the ventilation machine so on and another (SR Co.) did not it so. The former was examined at March 29th 1977, the latter was at June 28th 1976. But the respiratory communicable diseases, flue or common cold so on were not spreaded there at that time. The clinical aspects were compared between the group of SW, and SR. The swelling and hyperemic signs of nasal mucous membrane and the experience of nasal bleeding were about 50%, generally, in all the groups. The following problem was dizziness or vertigo. The nasal signs in the group of SW (improved ventilation of the room air) were relatively weak, but in another, it was some what severe; - there was necrotic sign with thick nasal clast. They were only used of gauze mask when the vapors of various solvents were deeply full in the room. And there was very high rate of bronchial signs, sputum or coughing in the group of SW improved ventilation so called, than another one. I suppose that it means chronic inflammatory change of the bronchial mucous membrane with deeper signs, due to the individual protectors were carelessly or not used according to the improving of the operating environment. Theses nasal signs mentioned the above were not nearly in the other groups had not been done the Chromium plating. The Status of RBC, Hb and Ht, of urine protein and urobilinogen were mostly in normal range. But the number of WBC was more or less showed with a positive correlation to the working duration.

온열작업장의 작업환경 개선대책

김두희,Kim, Du-Hui 대한산업보건협회 1986 산업보건소식 Vol.32 No.-

大邱市의 公害程度와 建康 : Ⅱ 大氣汚染과 建康

金斗熙 慶北大學校 醫科大學 1977 慶北醫大雜誌 Vol.18 No.1

대기중(大氣中) 배출(排出)된 일산화탄소(一酸化炭素)와 아황산(亞黃酸)가스의 지속시간(持續時間)

김두희,Kim, Doo-Hie 대한예방의학회 1974 예방의학회지 Vol.7 No.2

일산화(一酸化) 탄소(炭素)에 대한 피해와 대기오염(大氣汚染) 기준을 아황산(亞黃酸)까스를 기준으로 한다는 데의 관심을 가지고 가정에서 많이 사용하고 인는 연탄을 중심으로 관찰한 결과 CO는 연소온도와 비례하나 $SO_2$는 비례하지 않으며 일단 발생된 CO는 적어도 24시간(時間) 이내에는 변화가 거의 없으며 비교적 장시간 유지한다. $SO_2$는 대기중에서 곧 변화를 일으켜 ($H_{2}SO_{4}$로 전환) 30분이내에 극소량으로 되므로 대기중의 황화물 측정은 $SO_2$보다 유황자체나 $H_{2}SO_{4}$를 측정함이 더욱 효과적이라 하겠다. The concentration of Carton momoxide(CO) and Sulful dioxide($SO_2$) by burning time of coal, and its time of fading out in atmosphere after effluence from stove were measured with Kitagawa gas detector, January, 1974. Gas for measurement was sampled by gas syringe at the point distanced 60cm. from first junction of garvanized pipe of stove. CO concentration was directly proportional to the burning temperature of coal but $SO_2$ was generally constant durning burning time or also in proportion to burning temperature. CO gas of effluent mixed with air(relative humidity, 40%) was maintained for long period relatively, but $SO_2$ gas was early faded out within short time. The period decreasing to allowable limit was about 4 days in former and about 30 minutes in later, respectiively.

대구지방 산업장에 있어서 건강장애요인과 작업환경검사에 대한 기업인의 수용태도 (ll)

김두희,성수원,Kim, Du-Hui,Seong, Su-Won 대한산업보건협회 1988 산업보건 Vol.8 No.-

Examination of working environments was conducted to get more detailed data about harmful working environments and to make a contribution to more effective management. Study was carried out on 722 factories located in Taegu city and eight counties in Kyungpook Province, Korea, for a period of one year, from February 1 to December 30, 1986. The total number and proportion of workers exposed to harmful material was 37,697, 45.2% among 83,368 workers. The results according to exposed material were as follows: 1. In the case of noise, proportion of exceeding the 8-hour TLV was 59%, Included were nail-cutting in assembly metal manufacturing industry and weaving process in textile. 2. Dust in mill process of coal manufacturing industries exceeded the TLV of second class of dust at all parts and exceeded the TLV at 6% as a whole.: 3. The fields of industry lower than 70 lux of illumination were storage equipment of food, auto-winder of textile, painting of wood wares and coal mixing, and 44% of all cases was lower than standard. 4. As a result of temperature index investigation(WBGT), about 12% of all sujects exceeded limit value. Included parts were rolling machine and reducing room. 5. In the case of organic solvents, TLV was exceeded at about 8%, The parts exceeded TLV according to materials belonged to this category were as follows. 1) Toluene: adhesive work in assembly metal manufacturing 2) Xylene: printing and paint mixing in chemical manufacturing 3) Methyl ethytl ketone: paint mixing in all parts examined and coating machine partially in chemical manufacturing 4) Methyl isobutyl ketone: printing in chemical manufacturing 5) Acetone: vapor polishing in assembly metal manufacturing 6. Among specified chemical materials, the concentration of HC1 in the air in metal assembly manufacturing factory exceeded TLV. in one of three assembly metal manufacturing examined. Others, such as benzene, acetic acid, formic acid, sodium hydroxide, formalin, ammonia, copper, chromate etc. were lower than TLV in its indoor atmospheric concentration. As a whole, the proportion of exceeding TLV was about 0.8% 7. The concentrations of inorganic lead were lower than TLV in all parts examined. The results of this investigation show the fact that current management of working environments is not satisfactory, and so more active management is needed.