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      • SCOPUSKCI등재

        생쥐 수정란의 보조부화술에 있어서 Non-Contact Type인 Diode Laser의 이용

        김동훈,이명섭,강희규,한성원,김묘경,박원일,이훈택,정길생,이호준,Kim, D.H.,Lee, M.S.,Kang, H.G.,Han, S.W.,Kim, M.K.,Park, W.I.,Lee, H.T.,Chung, K.S.,Lee, H.J. 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.2

        The present study was performed to investigate the efficacy and safety of laser assisted hatching (AH) on mouse embryos. Non-contact $1.48{\mu}m$ diode laser system used to create a precise hole on zona pellucida. 2-cell embryos were collected from the mice (ICR) that had the coitus vaginal plug confirmed at 48 hours after hCG injection. Collected 2-cell embryos were cultured in the HTF medium supplemented with 0.4% BSA. For experiments, embryos at 8-cell stage were used after 18-22 hours in culture. After assisted hatching, the embryos were further cultured in HTF medium containing 0.1% PVP (anti-hatching system) for 3 days. For evaluate efficiency of laser on mouse embryo hatching, the effect of AH methods (acidic tyrode, pronase and laser), the number of artificial holes (1, 2 and 3 hole) and the irradiation time of laser (2, 4, 6, 8 and 10 ms) were examined. Hatching rates of laser AH group (95.2%) was significantly higher than that of control group (50.8%), but there was no differences among the laser (95.2%), acidic tyrode (100%) and pronase (98.5%) groups. Hatching rates of the number of zona pellucida opening by laser, there were no differences among the 1 hole (87.5%),2 hole (92.1%) and 3 hole (85.9%) groups. Developmental and hatching rates of embryos according to laser irradiation time were similar in the treatment groups. Therefore, these results suggest that laser AH using non-contact $1.48{\mu}m$ diode laser is a simple and accurate and effective procedure for AH. Based on these results, laser AH could be use safely for human ART program.

      • KCI우수등재

        수송과 계류가 도축전 돼지의 혈액성상에 미치는 영향

        김동훈,박구부,이제룡,주선태,문성실,김곤섭 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.5

        Changes in blood profile of pigs during transport and lairage were investigated. Six 110㎏ pigs(Landrace) were transported to slaughter house in a commercial livestock lorry for 1.5 hr then held in lairage for 3 hrs. Blood samples were taken from each pig at four times, pre-loading(pense), immediately after loading, immediately after transport and after 3 hrs lairage for analysis of blood profile. Cortisol and β-endorphin concentrations of immediately after transport were significantly higher (p$lt;0.45) than immediately after loading and pre-loading, but were significantly reduced (p$lt;0.05) by 3 hrs lairage to level of pre-loading. There were no differences in CPK (creative phosphokinase) levels between immediately after loading and transport, but the CPK levels were significantly higher (p$lt;0.05) than those of pre-loading. Also the CPK levels were significantly reduced by 3 hrs lairage, so that the CPK levels after 3 hrs lairage were not significantly different with that of pre-loading. These results suggested that pigs could have a physical stress during loading and physiological stress during transportation, and the stress could be recovered for 3 hrs of lairage. There were significant relationships between cortisol and β-endorphin (r=0.45, p$lt;0.05), CPK and β-endorphin (r=0.67, p$lt;0.01), LDH (lactate dehydrogenase) and CPK (r=0.67, p$lt;0.01).

      • 창업·창업벤처 중소기업의 조세지원제도에 관한 고찰

        김동훈 진주산업대학교 2001 論文集 Vol.40 No.-

        This study introduces those who begin business to the overview of current tax-aid systems for promoting the new businesses and new venture businesses in Korea. And this study emphasizes the characteristics and roles of new venture business in the standpoint of restructuring the industrial order and contributing to the national economic progress. Venture business is characterized by their small and medium size, high risk-high return, newness in age, new and high technology, new idea, high flexibility, entrepreneurship, and forntier spirit. And venture business plays an important role in improving technology and productivity, growing economic, vitalizing small-to-medium sized business, and so forth. On the other hand, the tax-aid systems for those who establish new businesses and new venture businesses in Korea include exemptions or reductions of national taxes such as corporation(individual) income tax, special tax for rural development, stamp tax, and non-taxable income and local taxes such as acquisitions tax, registration tax, property tax, and farmland tax.

      • 약관규제법의 시행과 정착과정에서의 주요 문제

        김동훈 한국소비자법학회 2017 소비자법연구 Vol.3 No.2

        The Standard Contract Terms Regulation Act (hereafter The Act) has been enforced since 1. Jul. 1987. Actually the main legal issues of the standard terms have not been discussed deeply in the field of scholarship and the cases have not been accumulated enough. Under this situation the Act seemed to be improvised according to extensive reception of foreign laws and some quantity of field research. But the steady effort of the committee of standard terms regulation has driven the court to accept gradually the main theories of standard terms. This enabled to build up some special legal theories of case law. At the same time the fair trade agency took up the regulation of standard terms and animated the administrative regulation. Now the 30-years old Act is playing a key role to protect consumers from unfair standard contract terms and advance the order of transaction. This article tries to overview the main issues since the enforcement of the Act. That includes the relation of regulation of standard terms and private autonomy, the relation of standard terms and individual agreement, regulation of unfair standard terms etc. Finally this article dealt with the incorporation of the Act into the civil code. 1987.7.1.자로 ‘약관의 규제에 관한 법률(이하 ’약관규제법‘으로 약칭)이 시행되기에 이르렀으나 실은 그 이전까지 약관법상의 여러 주요문제에 대하여 학계의 충분한 연구성과도 없었고 무엇보다 판례의 축적이 없는 상태에서 외국입법의 대폭적인 수용과 약간의 국내 거래실태 조사를 기반으로 급조된 감이 있는 약관규제법의 등장은 거래계나 법원에 대하여 별로 영향을 주지 못하였다. 그러나 약관심사위원회의 꾸준한 활동 등을 기초로 법원에서도 ‘예문해석’, ‘부동문자’등 종래의 판례이론에서 벗어나 약관의 특수한 법리를 정면으로 수용하기 시작하고 약관규제법을 판결의 근거로서 원용하기 시작하였다. 이제 약관규제법에 근거한 다수의 대법원 판례가 축적됨에 따라 특유한 판례이론의 형성 및 발전도 볼 수 있게 되었다. 또한 약관심사업무의 공정거래위원회로의 이관과 함께 약관문제에 대한 행정적 규제와 대응도 활발하여져서 매년 수백건의 약관심사청구가 이루어지고 표준약관도 활발히 보급되는 등 주목할 만한 발전을 이루어가고 있다. 이제 시행 30년을 맞이하는 약관규제법은 불공정한 약관의 사용으로부터 소비자를 보호함으로써 거래질서를 선진화하는 핵심적인 역할을 담당하고 있다. 이 글에서는 약관규제법 시행 30주년을 맞아 그간 동법의 시행 및 정착과정에서 주요한 쟁점이 되었던 문제들을 개관해보았다. 약관법의 기초로서 민법상사적 자치와의 관계에 관한 법리를 정리해보고, 실체법으로서 약관의 편입문제 및 개별약정과의 관계, 그리고 불공정약관의 통제와 관련하여서는 실무적 중요성이 부각된 위약금약정 문제를 살펴보았다. 그리고 절차법적 변천과정도 개관해보았다. 끝으로 약관규제법의 민법전 편입문제 등을 논하면서 동법의 앞으로의 발전방향을 전망해본다.

      • KCI등재

        NPPC 기준에 의한 돈육의 품질특성 연구

        김동훈,박범영,김일석,이무하,김용곤,이종문 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.5

        Two hundreds and twenty three pork carcasses of commercial breeds were studied for comparing to technical quality characteristics of pork identified by National Pork Producers Council(NPPC) scale. ? and conductance(C) were taken in 1(?_(1), C_(1)), 3(?_(3), C_93)), 6(?_(6), C_(6)) and 24hr(?u, Cu) postmortem on 4~5th thoracic vertebrae. Visual color and CIE value were estimated in longissimus dorsi(LD) muscle in 24hr postmortem on the two sites(4~5th thoracic vertebrae and last verlebrae) using National Pork Producers Council(NPPC) scale and color difference meter. Significant differences were observed in ?_(1), ?u, Cu, CIE L^(*) value, water holding capacity(WHC) and juiciness at all scales(p<0.05). But, ? and CIE L^(*) value were considerably affected by postmortem time and measuring site. NPPC scale was highly related with CIE L^(*)(R^(2)=0.77) and WHC(R^(2)=0.66), and moderately related with Cu((R^(2)=0.59) and ?u((R^(2)=0.54), and poorly related with?_(3)(R^(2)=0.32), ?_(6)(R^(2)=0.28) and C_(1) ~ C_(6)((R^(2)=0.03 ~ 0.18).

      • SCOPUSKCI등재

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