Gallium phosphate glass (GPG)를 첨가한 글라스아이오노머 시멘트의 기계적 성질과 항균효과

김동애(Dong-Ae Kim),김규리(Gyu-Ri Kim),이정환(Jung-Hwan Lee),이해형(Hae-Hyoung Lee) 대한치과재료학회 2019 대한치과재료학회지 Vol.46 No.4

This study investigated the mechanical properties and antibacterial activity of glass ionomer cement (GIC) containing 1 mol% or 5 mol% gallium phosphate glass (GPG) powder. GPG were added to conventional GIC powder in a range of 0~20 wt%. The specimens for compressive strength (4 mmØ×6 mm) and diametral tensile strength (6 mmØ×4 mm) were prepared by mixing with the cement liquid and kept in an water of 37±1℃. Mechanical properties were evaluated at an interval of 1 h, 24 h, and 7 days. Antibacterial activity of specimens against to S. mutans and S. sobrinus was evaluated using agar diffusion methods. Data were statistically analyzed by one-way ANOVA and Tukey HSD post-hoc test (p<0.05). Diametral tensile strength of GIC containing GPG were significantly higher than control GIC while compressive strength showed no statistical difference. There was an increasing tendency of antibacterial activity for GPG-GICs. Results indicated that GPG can be considered as potential reinforcing agent for increasing antibacterial and mechanical properties for conventional GIC.

탄소나노튜브 첨가에 의한 치과용 글라스아이오노머 시멘트의 기계적 특성

김동애(Dong-Ae Kim),김한샘(Han-Sem Kim),신원상(Ueon-Sang Shin),이해형(Hae-Hyoung Lee) 대한치과재료학회 2016 대한치과재료학회지 Vol.43 No.1

The aim of this study was to investigate the effect of multiwall carbon nanotube functionalized with carboxyl group (MWCNT-COOH) on the mechanical properties of dental glassionomer cement (GIC). MWCNT-COOH was prepared by the acid oxidative method. The MWCNT-COOH was incorporated into a commercial GIC powder or liquid at 0.5 wt% or 1.0 wt%. The net setting time of the cements was measured in accordance with ISO 9917 (Dental water-based cement). Specimens for compressive strength (4 mmφ × 6 mm), diametral tensile strength (6 mmφ × 4 mm) and flexure strength with modulus (2 mm × 2 mm × 25 mm) were prepared by mixing with the cement liquid and kept in water bath of (37±1)℃. Mechanical tests were conducted in 1 d, 7 d, and 14 days at a cross-head speed of 1 mm/min. Compressive strength of GIC mixed with 0.5 wt% MWCNT-COOH increased significantly at 7 d. However, overall mechanical properties of GIC modified with MWCNT were not significantly increased with a delayed setting time, in comparison with control cement. Overall results indicated that the MWCNT/GIC composite cements showed a limited strengthening effect for dental glassionomer cement.

티타니아 나노섬유 첨가에 의한 치과용 글라스아이오노머 시멘트의 강화

김동애(Dong-Ae Kim),김규리(Gyu-Ri Kim),송찬근(Chan-Keun Song),윤종원(Jong-Won Yoon),이해형(Hae-Hyoung Lee) 대한치과재료학회 2012 대한치과재료학회지 Vol.39 No.3

The aim of this study was to investigate the mechanical properties of dental glass-ionomer cement containing titania nanofiber (TNF). The TNF was fabricated by electrospinning using chemicals and calcinated at 800℃, and mixed with a commercial glass ionomer powder by ball milling in 10 wt%. Specimens for compressive strength (φ4 ㎜ x 6 ㎜) and diametral tensile strength (φ6 ㎜ x 4 ㎜) were prepared by mixing with the cement liquid and kept in an water of (37±1)℃. Mechanical strength tests were conducted at 1 h, 24 h, and 7 days at cross-head speed of 1 ㎜/min. Data were statistically analyzed by one-way ANOVA and Tukey HSD post-hoc test. Mean strength values of GIC containing TNF was significantly higher than unmodified or ball milled groups without TNF (p<0.05). Scanning electron micrographs showed that TNF was embedded and bonded with the cement matrix. Results indicated that the TNF was considered as potential reinforcing material for dental glass-ionomer cement.

여러 가지 광원에 의하여 중합된 치과용 콤포짓트 레진의 바콜 경도

김동애(Dong-Ae Kim),전수경(Soo-Kyung Jun),구효진(Hyo-Jin Kn),이해형(Hae-Hyoung Lee) 대한치과재료학회 2006 대한치과재료학회지 Vol.33 No.2

저/고분자량 키토산에 의한 종래형 치과용 글라스아이오노머 시멘트의 강화

김동애(Dong-Ae Kim),김규리(Gyu-Ri Kim),전수경(Soo-Kyung Jun),이정환(Jung-Hwan Lee),이해형(Hae-Hyoung Lee) 대한치과재료학회 2017 대한치과재료학회지 Vol.44 No.1

The aim of this study was to investigate the effects of chitosan powder addition on the strengthening of conventional glass ionomer cement. Two types of chitosan powders with different molecular weight were mixed with conventional glass ionomer cement (GIC): low-molecular weight chitosan (CL; 50∼190 kDa), high-molecular weight chitosan (CH; 310∼375 kDa). The chitosan powders (CL and CH) were separately added into the GIC liquid (0.25-0.5 wt%) under magnetic stirring, or mixed with the GIC powder by ball-milling for 24 h using zirconia balls. The mixing ratio of prepared cement was 2:1 for powder to liquid. Net setting time of cements was measured by ISO 9917-1. The specimens for the compressive strength (CS; 4 x 6 mm), diametral tensile strength (DTS; 6 x 4 mm), three-point flexure (FS; 2 x 2 x 25 mm) with flexure modulus (FM) were obtained from cements at 1, 7, and 14 days after storing in distilled water at (37±1)℃. All mechanical strength tests were conducted with a cross-head speed of 1 mm/min. Data were statistically analyzed by one-way ANOVA and Tukey HSD post-hoc test. The mechanical properties of conventional glass ionomer cement was significantly enhanced by addition of 0.5 wt% CL to cement liquid (CS, DTS), or by addition of 10 wt% CH (FS) to cement powder. The CL particles incorporated into the set cement were firmly bonded to the GIC matrix (SEM). Within the limitation of this study, the results indicated that chitosan powders can be successfully added to enhance the mechanical properties of conventional GIC.

β-TCP 첨가량에 따른 생분해성 PLA/β-TCP 복합체의 생체활성과 기계적 특성 평가

문희정,김설악,김동애,Moon, Hee-Jung,Kim, Seol-Ak,Kim, Dong-Ae 한국콘텐츠학회 2021 한국콘텐츠학회논문지 Vol.21 No.3

본 연구는 PLA에 β-TCP를 첨가한 복합체의 생체활성도와 기계적 성질을 평가하고자 한다. 클로로프롬 용액에 24 시간 교반기를 이용해서 PLA와 β-TCP를 무게별로 0, 10, 30, 50 wt% 용해한 후 에탄올 용액에서 PLA/β-TCP 복합체를 추출 후 사출성형을 통해 다양한 기계적 평가를 위한 시편을 제작하였다. SBF 인체 유사 체액에 1, 7, 30, 90 일 동안 침전하여 생체활성도를 평가하였다. 본 연구 결과 PLA/β-TCP 10-30 wt% 첨가한 실험군에서는 유의하게 강도가 증가하였으나 50 wt% 첨가한 실험군에서는 감소하였다. 결론적으로 β-TCP 30 wt% 이상 첨가하는 것은 사출성형 고분자 복합체의 기계적 특성이 개선되지 않는다. 표면 생체활성도는 90일 침전 후 표면 전체에 아파타이트 결정을 형성하였다. β-TCP는 표면특성과 기계적 성질 개선효과가 있음을 입증하였다. 향후 PLA/β-TCP 첨가량에 따른 효율성과 폭 넓은 물성 연구가 필요할 것으로 사료된다. The aim of the present study was to investigate the mechanical properties of melt-injected poly lactic acid (PLA) composites with β-tricalcium phosphate (β-TCP). The PLA mixed with calcined PLA/β-TCP powder to be contents of 0, 10, 30, 50 wt%, respectively, was dissolved in chloroform solvent under stirring for 24 h. Then the liquid mixtures were dropped into ethanol to extract solvent. After drying, the well-dispersed PLA/β-TCP composites were granulated and melt-injected to prepare specimens for various mechanical testing. PLA/β-TCP induced the precipitation of an apatite bone-mineral phase on the surface after immersion in a human simulated body fluid (SBF) for 90 days, showing in bioactivity. Mean various mechanical properties PLA/β-TCP composite were increased up to 10-30 wt% with significantly in part and composite were decreased 50 wt% of showing in mechanical properties. In conclusion, Over 30 wt% addition of β-TCP to PLA may be not advisable to improve the mechanical properties of melt-injected polymeric composites. Results indicated that β-TCP can be used considered as potential reinforcing agent for increasing mechanical properties for PLA. Therefore, it was suggest that the additional effects of β-TCP and research on a wide range of substances.

기능화 다중벽 탄소나노튜브를 첨가한 글라스아이오노머 시멘트의 항균효과 및 기계적 성질

정미애(Mi-Ae Jeong),김동애(Dong-Ae Kim) 한국콘텐츠학회 2017 한국콘텐츠학회논문지 Vol.17 No.10

본 연구는 상업용 글라스아이오노머 시멘트(GIC) 분말에 기능화 다중벽 탄소나노튜브(MWCNT)를 첨가하여 항균효과와 기계적 성질을 개선하고자 하였다. MWCNT를 기존의 GIC 분말에 0.125, 0.25, 0.5와 1.0wt%를 첨가하여 조성하였다. 비커스 경도(VHN)는 (Ø10 mm x 2 mm) 각각 4개의 시편을 제작하여 증류수에 1일, 7일, 14일 보관 후 200 N 하중에서 20초 유지시간으로 경도를 측정하였으며 항균효과는 S. mutans와 S. aureus균주를 아가 디스크 확산법으로 억제대를 측정하여 평가하였다. 실험 결과 비커스 경도는 MWCNT를 첨가한 실험군에서 높은값을 보였으며 MWCNT를 첨가한 모든 실험군에서 침전시간이 증가할수록 유의하게 높은 경도값을 보였다(p<0.05). 항균실험은 S. mutans균에서 다소 높은 항균효과를 보였으나 통계적으로 유의한 차이는 나타나지 않았으며, S. aureus균은 MWCNT를 0.25 wt%첨가한 군에서 통계적으로 유의한 차이점을 보였다(p<0.05). 이상의 결과로 MWCNT를 첨가한 GIC는 항균효과와 기계적 성질에 효과가 있음을 입증하였다. 추후 MWCNT의 효율적인 기능화 처리에 대한 연구가 필요할 것으로 사료된다. The purpose of this study was to investigate the antibacterial effect of functional multiwallcarbon nanotube (MWCNT) on the conventional glass ionomer cement(GIC). The MWCNT wasincorporated into a commercial powder at 0.125, 0.25, 0.5 and 1.0 wt%. Specimens for vickershardness(VHN) disks(Ø10 ㎜ x 2 ㎜) each were prepared. Indention were made using a loadof 200 N and 20 s dwell on four specimens for each distilled water storage time 1d, 7d, and14days. The antibacterial effects using agar diffusion test with S. mutans and S. aureus. Thediameters of the inhibition zones produced around the materials were measured. The resultsrevealed that all the vickers hardness values were increase significantly with incubationtime(p<0.05). Regarding the antibacterial effect for S. mutans, all the tested groups showed aslightly higher value not significantly with the control group(p<0.05). However S. aureusstatistical analysis indicated a significant difference for antibacterial agents between control andMWCNT containing 0.25 wt%(p<0.05). These results of this study provide that the conventionalGIC with containing MWCNT show good antibacterial effect against and favorable mechanicalbe needed.

세륨옥사이드나노입자(Cerium Oxide Nano Particles; CNPs)를 첨가한 치면열구전색재의 물리적 특성 및 세포독성

정미애(Mi-Ae Jeong),김동애(Dong-Ae Kim) 한국콘텐츠학회 2022 한국콘텐츠학회논문지 Vol.22 No.3

본 연구는 기존의 치면열구전색재 ConciseTM에 세륨옥사이드 나노입자(Cerium oxide nano particles; CNPs)를 0-4.0 wt%를 첨가하여 새로운 치면열구전색재를 제조하여 물리적 성질과 세포독성을 평가하였다. 물리적 성질은 중합깊이, 물흡수도와 용해도를 측정하였고 세포독성평가는 불멸화된 구강점막세포(Immortalized human oral keratinocyte (IHOK))를 이용하여 MTT assay법으로 평가하였다. 실험 결과 중합 깊이는 CNPs 첨가량이 증가할수록 감소하였고, 용해도는 CNPs를 2.0 wt% 첨가된 실험군에서 가장 낮은 값을 보였으며 물흡수도는 각 실험군별 유의한 차이는 나타나지 않았다(p >0.05). 세포독성 실험 결과는 모든 실험군에서 높은 세포 생존율을 보였다. 이는 CNPs가 물리적 성질을 크게 약화 시키지 않으며 세포독성을 나타내지 않았으므로 생체적합성을 입증하였다. CNPs의 특성을 고려하여 향후 CNPs의 효율적인 분산 기술에 대한 추가 연구가 필요할 것으로 사료된다. In this study cerium nano particles(CNPs) with 0-4.0 wt% was incorporated to the conventional dental pit and fissure sealant(ConciseTM) to produce new pit and fissure sealant the physical properties and cytotoxicity. The physical properties were measured for polymerizing depth the degree of water absorption and solubility. The cytotoxicity of cell viability was analyzed by MTT assay using immortalized human oral keratinocyte(IHOK). As a result of this preceding study the polymerizing depth was decreased by the increasing of the amount of CNPs. The solubility degree of the sealant added CNPs with 2.0 wt% showed was the lower and the water absorption showed no significantly difference with the control groups(p >0.05). The cytotoxicity test results showed high survival rates in all experimental groups. Therefore, pit and fissure sealant by the addition of CNPs excellent cell viability be produced without weaken the physical property of the cell viability fissure sealant containing CNPs does not weaken physical properties and has no cytotoxic effects biocompatibility. Considering its properties effect of CNPs, further studies are required for distribution technology application.

압입 하중과 침적 기간에 따른 의치상용 레진의 비커스 경도

김규리(Gyu-Ri Kim),김동애(Dong-Ae Kim),김남석(Nam-Suck Kim),양태현(Tae-Hyun Yang),이해형(Hae-Hyoung Lee) 대한치과재료학회 2014 대한치과재료학회지 Vol.41 No.3

The purpose of this study was to examine the effects of indentation load and storage in water on Vickers hardness and viscoelastic recovery of indentation of denture base resins. Two types of acrylic denture base materials were investigated; Vertex (VT) and Acryshot (AS). Five resin disks each were prepared in 20 mm diameter and 1.8 mm in thickness) and highly polished according to manufactures recommendations. Vickers hardness test was conducted at a load of 25, 250, 500, 1000, 2000 gf with a dwell time of 30 s from one specimen. Vickers hardness (HV) and maximum indentation depth values at each load were averaged from three measurements. Vickers diagonal length of each indentation was re-measured after water storage of 1, 3, and 7 days, and HV values of specimens were recalculated. There were no significant differences in HV between indention loads (p>0.05), indicating no load dependency in HV value of specimens. Moreover, the HV value of specimens did not show statistically significant change after water storage up to 7 days. However, the indentation depth (D<SUB>m</SUB>) values were higher than the indentation depth (D<SUB>c</SUB> ) calculated from Vickers indentaiton geometry. The difference between D<SUB>m</SUB> and D<SUB>c</SUB> was increased with increase in indentation load, indicating some load dependency. This phenomenon was probably due to immediate viscoelastic recovery of denture base resins. Vickers hardness with indentation depth measurement can be a useful tool to investigate mechanical behavior of denture base resins.

다중벽 탄소나노튜브를 첨가한 글라스아이오노머시멘트의 항균효과 및 기계적 특성

정미애(Jeong mi- ae),김동애(Kim dong-ae) 한국콘텐츠학회 2017 한국콘텐츠학회 종합학술대회 논문집 Vol.2017 No.5