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17Beta-Estradiol Inhibits Calcium-Activated Potassium Channel Expressions in Rat Whole Bladder
김덕윤,양은경 대한배뇨장애요실금학회 2016 International Neurourology Journal Vol.20 No.1
Purpose: To investigate the effect of estrogen on the expression of calcium-activated potassium (KCa) channels in an overactive bladder rat model. To this end, mRNA and protein levels of KCa channel subtypes in the bladder of ovariectomized rats were measured by reverse transcription polymerase chain reaction and western blotting, respectively. Methods: Ten-week-old female Sprague-Dawley rats were divided randomly into 3 groups: sham-operated control group (n=11), ovariectomy group (n=11), and the group treated with estrogen after ovariectomy (n=12). Rats in the last group were subcutaneously injected with 17β-estradiol (50 μg/kg) every other day for 2 weeks, whereas rats in the other 2 groups received vehicle (soybean oil) alone. Two weeks after treatment, the whole bladder was excised for mRNA and protein measurements. Results: Protein levels of the large-conductance KCa (BK) channels in the ovariectomy group were 1.5 folds higher than those in the sham-operated control group. However, the protein levels of the other KCa channel subtypes did not change significantly upon bilateral ovariectomy. Treatment with 17β-estradiol after ovariectomy restored BK channel protein levels to the control value. In contrast, BK channel mRNA levels were not significantly affected by either ovariectomy alone or 17β-estradiol treatment. The small-conductance KCa type 3 channel (SK3) mRNA and protein levels decreased to 75% of control levels upon 17β-estradiol treatment. Conclusions: These results suggest that 17β-estradiol may influence urinary bladder function by modulating BK and SK3 channel expression.
김덕윤,권오대,김상경,신임희 대한배뇨장애요실금학회 2008 International Neurourology Journal Vol.12 No.1
Purpose: International guidelines and national laws have been formulated to protect human subjects in research. Recently, many efforts have been tried to contribute to better awareness and knowledge about the link between quality systems and protection to develop better quality procedures in health research. In this study, to improve the understandings about health research, awareness about health research was evaluated with questionnaires for patients and care-givers in our institute. Materials and Methods: 339 persons responded to the questionnaires. 128 persons were patients with mild symptoms and 69 were caregivers. 123 were male and 179 were female. Dominant educational level was college graduates and were 166 and economic status was middle-class (247). Questionnaires was composed of 18 items; 7 demographical characteristics items, 7 understanding of clinical trials items, 4 understandings of IRB (Institutional Review Board) concepts items. Results: 71.7% of responder have informations about clinical trials by TV and 23.9% by newspapers. 22.3% have never heard about clinical trials. Knowledge level about clinical trials was thought to be a middle level and subjects of clinical trials were animals (62.5%), patients (54.9%), normal persons (25.4%), plants (11.8%). Objects to be included in clinical trials were pharmaceuticals (62.2%), cell therapy (58.7%), medical device (30.4%), health promotion foods (14.5%). Answer about 'Intention to be the participant" were no in 56% and yes in 34.5%. Conclusions: Although mass media contributed to the spread of understandings about clinical trials, 22.3% did not know about health research. To improve the awareness and understandings about health research, television (TV) can be the most effective method and the safety about human subject protection should be certified. (J Korean Continence Soc 2008;12:48-57)
방광암 진단을 위한 세 가지 요채집법에 따른 ThinPrep 검사와Melanoma Antigen Gene 역전사 중합효소연쇄반응법의 검출성적 비교
김덕윤,조혁진,오훈규,신임희,전창호 대한진단검사의학회 2007 Annals of Laboratory Medicine Vol.27 No.1
배경 : 저자들은 방광암 환자와 대조군의 배뇨, 배출뇨, 세척뇨를 채취하여 ThinPrep 검사와 MAGE 검사의 민감도와 특이도를 조사하였다.방법 : 방광암 환자 20명과 대조군 10명으로부터 수술 전 아침첫 배뇨를 채취하였고 또한 배출뇨와 세척뇨를 각각 채취하였다.채취한 소변은 2개의 시험관에 균등하게 나누어져 ThinPrep 검사와 MAGE 검사를 각 시행하였다. 방광암 존재의 확인은 방광경 소견을 기준으로 하였다. ThinPrep 검사와 MAGE 검사의결과는 종양의 분화도와 병기에 따라 비교하였다.