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김대재 충북대학교 과학교육연구소 2003 과학교육연구논총 Vol.19 No.2
Full scale of cucumber genomic library has been successfully constructed using Lambda Fix II vector system for any genes from cucumber. The genomic library screening produced full scale of cucumber PCK gene clone (named as λspck-4A) and the λcspck-4A clone has been intensively examined by restriction enzyme analysis and Southern blot analysis. After sub-cloning of the insert DNA fragments into plasmid vector, full length of cucumber PCK gene sequence was obtained by DNA sequencing using primer walking technology. This is the first successful cloning of PCK gene from higher plants in C3 photosynthetic class. Therefore, the results will be useful for the study of the PCK gene expression and regulation in cucumber development.
김대재 한국생명과학회 2023 생명과학회지 Vol.33 No.8
Peroxisomes, known as microbodies, are a class of morphologically similar subcellular organelles commonly found in most eukaryotic cells. They are 0.2~1.8 µm in diameter and are bound by a single membrane. The matrix is usually finely granular, but occasionally crystalline or fibrillary inclusions are observed. They characteristically contain hydrogen peroxide (H2O2) generating oxidases and contain the enzyme catalase, thus confining the metabolism of the poisonous H2O2 within these organelles. Therefore, the eukaryotic organelles are greatly dynamic both in morphology and metabolism. Plant peroxisomes, in particular, are associated with numerous metabolic processes, including β-oxidation, the glyoxylate cycle and photorespiration. Furthermore, plant peroxisomes are involved in development, along with responses to stresses such as the synthesis of important phytohormones of auxins, salicylic acid and jasmonic acids. In the past few decades substantial progress has been made in the study of peroxisome biogenesis in eukaryotic organisms, mainly in animals and yeasts. Advancement of sophisticated techniques in molecular biology and widening of the range of genomic applications have led to the identification of most peroxisomal genes and proteins (peroxins, PEXs). Furthermore, recent applications of proteome study have produced fundamental information on biogenesis in plant peroxisomes, together with improving our understanding of peroxisomal protein targeting, regulation, and degradation. Nonetheless, despite this progress in peroxisome development, much remains to be explained about how peroxisomes originate from the endoplasmic reticulum (ER), then assemble and divide. Peroxisomes perform dynamic roles in many phases of plant development, and in this review, we focus on the latest progress in furthering our understanding of plant peroxisome functions, biogenesis, and dynamics.
오이의 발달에서 노쇠화와 관련된 metalloproteinase 유전자
김대재 충북대학교 과학교육연구소 1999 과학교육연구논총 Vol.15 No.1
Cucumber cotyledons provide an excellent experimental system in which to investigate developmental changes in gene expression from an early phase of heterotrophic growth (germination) through phototrophic growth to senescence. A cDNA library from mRNA of senescent cotyledons ($gt;95% yellow) has been constructed. Differential screening of this cDNA library allowed the identification of two senescence-related cDNAs (SE71 and SE74). SE71 encodes a putative SPF1-type DNA binding protein. The SE74 gene encodes a pre-pro-enzyme which has the hallmark motifs of the metzincin superfamily of zinc metalloproteinases, in particular the matrix metalloproteinase family (MMPs). Although mammalian MMPs have been implicated in such processes as extracellular matrix degradation during cell migration in normal (e.g. embryogenesis, fusion of gametes) or pathological (e.g. macrophages, metastasis) situations, the function of the soybean enzyme, first described in higher plants in unclear. Current data brings new insights on the physiological function of this plant enzyme, particulary in the senescence of cucumner cotyledon.
김대재,도택환,이규진 한국통합생물학회 2005 Animal cells and systems Vol.9 No.3
We investigated expression of cucumber senescence-associated genes (SAGs) from developing cotyledons and flower petals. Several cucumber SAGs have been reported in earlier reports. This is an extension of the previous findings. Semi-quantitative RT-PCR revealed that most of the cucumber SAG transcripts were consistently produced until the organ senescence. These results imply that many cucumber senescence-related genes are still active during the final development stage, playing some executive biological roles, possibly in remobilization of nutrients to the other parts of tissues or organs. These results were used to search for possible functions of senescence-related genes during organ development.
김대재 충북대학교 과학교육연구소 1998 과학교육연구논총 Vol.14 No.1
The science of genetics is the study of inheritance. There has been great progress in genetic technology for the last two decades which is awesome development in 20 century's science. We now know that many genetic diseases are originated from molecular level. Development in genetics are already having practical consequences in clinical medicine, pharmaceutical production, animal experimentation, agriculture and industry. The potential applications seem limitless for the next half century. The possibilities and implications of the 'new genetics' are awesome but we lack social and ethical guide-lines to lead us through this unknown world. Some people have raised questions about the consequences of applying our new knowledge fearing unwelcome or catastrophic outcomes. In this brief report, potential problems and matters are discussed following science of genetics developments.
담배 야생종의 원형질체 배양에 미치는 무기영양소의 효과
김대재(Dae Jae Kim),박민철(Min Chul Park),김상구(Sang Gu Kim) 한국식물학회 1988 Journal of Plant Biology Vol.31 No.4
Leaf mesophyll protoplast cultures from six Nicotiana species, N. debneyi, N. rustica, N. amplexicaulis, N. glauca, N. glutinosa, and N. sylvestris were carried out. When we reduced the NH_4NO_3 and Fe·EDTA concentration to 1/3(7 mM) and 1/10(10 μM) from the Murashige and Skoog medium respectively, cell division of the protoplasts was efficiently induced in four Nicotiana species, N. debneyi, N. rustica, N. amplexicaulis and N. glauca. However, other two species, N. glutinosa and N. sylvestris were failed in inducing cell division at the same culture condition. The protoclone calluses derived from four Nicotiana speices were consequently regenerated on a MS basal medium supplemented with the appropriate auxin and cytokinin.