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다문화 가정의 이중 언어 사용 학생지도를 위한 중등교사 연수방안
권영수 미래영어영문학회 2012 영어영문학 Vol.17 No.3
Recent surveys show that the students from multi-cultural families experience problems of language barriers, low academic performance, school bullying, etc. As they pass puberty, these problems might affect their emotional as well as intellectual development, inducing the serious issues in our society. To prevent such deterioration, we should provide a special program for middle or high school students, which is helpful for their academic performances as well as their emotional stability. This study aims to propose a workshop program for middle or high school teachers who have the potential ability to guide and teach the multi-cultural students in local areas. The language teachers with their linguistic knowledge and their teaching experience can be potential mentors for the students. The program suggested in this study can be a pilot case for extended multi-cultural education.
Analysis of microRNAs in a knock-in hESC line expressing epitope-tagged AGO2
권영수,송호석 한국통합생물학회 2016 Animal cells and systems Vol.20 No.1
Argonaute (AGO) family proteins, which are key components of RNA-induced silencing complexes (RISCs), associate with both microRNAs (miRNAs) and mRNAs to regulate gene expression at posttranscriptional stages. While high-quality antibodies for AGO proteins are extremely useful for studies on the functions of miRNAs and AGO proteins, they are not always readily available. In this study, we generated a knock-in human embryonic stem cell (hESC) line by using homologous recombination to express FLAG-tagged AGO2 from its native genomic locus. FLAGtagged AGO2 was exploited to analyze AGO2-bound miRNAs in hESCs cultured on a feeder layer by immunoprecipitation. Furthermore, the pluripotency of ESCs allowed for the analysis of miRNAs after differentiation into fibroblasts and cardiomyocytes. The genetically modified hESC line generated in this study will be highly useful for studying the functions of miRNA-mediated regulation in human development and cell differentiation.