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      저자 : 구헌우 ( Heon-woo Ku ) (검색결과 1 건)
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      • 벼 세균성 흰잎마름병(Xanthomonas Oryzae pv. Oryzae)의 생물학적 방제를 위한 미생물 사용방법

        정종국 ( Jong-kook Jung ),서민경 ( Min-kyoung Seo ),양현주 ( Hyun-joo Yang ),구헌우 ( Heon-woo Ku ),남효송 ( Hyo-song Nam ) 한국환경농학회 2021 한국환경농학회 학술대회집 Vol.2021 No.-

        The purpose of this study is to develop eco-friendly microbial agents and application technologies for the safe production of rise by control of rice Bacterial Blight (Xanthomonas oryzae pv. oryzae). Rice seeds (Oryza sativa cv. ShinDongjin) were inoculated with 3 types of microorganisms (Bacillus amyloliquefaciens KB3, Bacillus velezensis LM11, Serratia spp IM2), chemical disinfectant (tebuconazole 12.5 + prochloraz copper chloride 12.5%) and Trichoderma at 25°C for 24 hours. After inoculation, the rice seeds were washed twice with distilled water, placed on PDA medium, and incubated at 25°C for 48 hours. As a result of the culture, microorganisms causing white leaf blight were cultured in the rice seed treated with chemical disinfectant, and each strain was cultured in the rice seed inoculated with Bacillus amyloliquefaciens KB3, Bacillus velezensis LM11, and Trichoderma. The germination rate of the seed inoculated with each sample was confirmed. In the case of rice seeds treated with microorganisms and Trichoderma, the germination rate was about 3.5 times higher than that of chemical disinfectants. As a result of checking the growth level of each sample 2 weeks after sowing, it was confirmed that the Bacillus velezensis LM11 inoculated sample had a higher growth rate than other samples. Each sample was transplanted into a Wagner pot and growth was investigated 2 weeks after transplantation. As a result, the sample inoculated with Bacillus velezensis LM11 showed a higher growth rate than the other samples, and the rest of the samples grew similarly. Each rice seed was immersed in the sample to infect the grown rice with Xanthomonas oryzae pv. oryzae, which causes white leaf blight. As a result of infection, it was confirmed that the infection rate was the lowest in Bacillus velezensis LM11.

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