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겔스캐너를 이용한 변성아크릴아마이드 겔의 형광 DNA 검출
구자환,정지웅,조영찬 한국작물학회 2005 Korean journal of crop science Vol.50 No.S
형광 염료와 레이저 겔스캐너 장비를 이용하여 변성아크릴 아마이드 겔에서 전기 영동된 DNA를 신속하고 간편한 방법으로 기존의 은염색법과 비슷한 감도로 검출하고자 하였다. 변성아크릴아마이드 겔을 형광 염료인 SYBR Green (Molecular Probes)이나 Vistra Green (Amersham Bioscience) 0.01 X 희석액 (pH 8)으로 염색한 후 480nm 레이져, 520nm filer 옵션으로 스캔하여 DNA를 검출하였으며, 검출감도는 기존의 은염색법과 비슷하면서 염색 단계를 한 단계로 줄일 수 있었다. The denature polyacrylamide gel stain silver nitrate is used for routine nucleic acid detection. More sensitive stains, such as Vistra Green, SYBR Green are available to address a broad range of DNA applications requiring lower detection limits in polyacrylamide gel formats. Gel Scanners, laser-scanning instruments, provide sensitive fluorescence detection of DNA gel stains. We established one step fluorescent impregnation enhanced sensitivity with simple, rapid and low cost. We have applied this fluorescent staining procedure for the routine analysis of DNA profiles generated by SSR amplification.
Steel-Wood 합성보의 내력-변형 특성에 관한 실험적 연구
구자환,박학길 대한건축학회 2000 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.20 No.2
This experimental study is on the structural behavior and the bending capacity of steel-wood composite beam (SWC). SWC insert wood in two Lip-C channel which symmetric bending axis and combine in a bolt. Four specimens were made with three different types of section shape. Section shapes are two Lip-C channels which symmetric for bending axis and two different wood sections. lure bending moment are only considered for loading condition. The test results lead to the next conclusions. (1) The specimens SWC has more bending capacity than Lip-C channel. (2) When SWC is loaded, expect a part wood rupture does not occur because of composite effect.
구자환,김민태,오인석,김재덕,강위금 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04
국내에서 녹비작물로 이용되는 헤어리베치의 채종용 지지식물로 적합한 밀 품종을 선발하고자 국내에서 장려되거나 재배되었던 금강밀을 포함한 33개 밀 품종을 대상으로 지지효과를 시험하였다. 수원에 위치한 국립식량과학원 시험포장에서 2008년 10월 8일에 보라 헤어리베치와 밀을 3:1 비율로 혼합하여 4 kg/10a을 50cm 간격으로 조파 재배하여 헤어리베치 종자 수확 시기에 도복 정도를 조사한 결과 올밀, 조은밀, 금강밀 은 도복 정도가 4~5 정도로 6 이상을 나타 낸 다른 밀 품종에 비해 지지 효과가 우수하였고 헤어리베치 종자 수량도 조은밀 95kg/10a, 금강밀 94kg/10a, 올밀 86kg/10a로 다른 밀 품종에 비해 헤어리베치 종자 수량 이 높게 나타났다.
방광암의 진단에 이용한 Bladder Tumor Antigen(BTA) Stat Test와 요세포검사와의 비교
구자환,이창규,류현열 고신대학교 의학부 2002 高神大學校 醫學部 論文集 Vol.17 No.1
Purpose We assessed the sensitivity and specificity of the noninvasive BTA stat test for detection of bladder cancer with special reference to the grades and stages of the tumors. Materials and Methods A total of 163 patients provided voided urine samples for the BTA stat test and cytopathological study. Each patient underwent cystoscopy. Transurethral resections were performed when a bladder tumor was seen or cystoscopic biopsies were obtained when a carcinoma in situ was suspected. Sensitivity was determined using voided urine samples from patients with histologically proven bladder cancer (n=125). Specificity was determined from patients with benign urologic disease (n=40), healthy volunteers (KTP donor, n=20), malignant urologic cancer other than bladder (n=18), non-urologic malignancy (n=2), and patients, with a history of bladder cancer but free of disease (n=24). The sensitivity and specificity of BTA stat test were compared to those of standard voided urine cytology, and the sensitivities of BTA stat test and urine cytology were stratified according to histologic grades and stages. Results Overall sensitivity of the BTA stat test and urine cytology were 86.6% and 45.2% specificity were 72.3% and 97.3% respectively. For each histologic grade of transitional cell carcinoma (grade 1, 2, 3, 4), sensitivity was 36.4%, 84.1%, 87.2% and 91.3% for BTA stat test, and 18.2%, 13.6%, 53.2% and 82.6% for urine cytology, respectively. For each stage of transitional cell carcinoma (stage Ta, T1 > T2), sensitivity was 50%, 81.5% and 98.4% for BTA stat test, and 0%, 5.6% and 75.4% for urine cytology, respectively. The false positive of BTA stat test was seen in patients with gross hematuria, urinary tract infection, urologic malignancy other than bladder cancer and benign prostatic hyperplasia. Conclusion BTA stat test is a simple, non-invasive, inexpensive adjunct to cystoscopy with urine cytology. In our study the BTA stat test was clinically reliable in the diagnosis for bladder cancer, and can be used as a screening test for bladder cancer in patients with hematuria or irritative voiding symptoms.
구자환,한옥규,손범영,이진석,김정태,김대욱,이유영,김민태,문중경,황종진,김충국,백성범,권영업 한국육종학회 2013 한국육종학회지 Vol.45 No.3
헤어리베치의 꽃색 중 보라색, 분홍색, 백색의 유전 양상을 구명하기 위하여 보라색 꽃 품종(청풍보라), 분홍색 꽃 품종(Mamecho) 그리고 백색 꽃 품종(HV-Kw1) 상호 간에 교잡하여 잡종 2세대(F2)에서 꽃색 분리 비율을 조사하고, 또한 분홍색과 연분홍색 사이의 유전 양상을 조사하기 위하여 연분홍색 꽃 품종(HV-Mp1)과 분홍색 꽃 품종(Mamecho)을 교잡하여 F2에서 꽃색 분리 비율을 조사한 결과 백색/보라색 조합과, 보라색/분홍색 조합에서 보라색은 백색과 분홍색에 대해 3 : 1의 비율로 분리하였고, 연분홍색/분홍색 조합에서 분홍색 3, 연분홍색 1의 비율로 분리하였으나, 백색/분홍색 조합의 F2 집단에서는 보라색 9, 분홍색 3, 백색 4의 비율로 분리하였다. 이러한 헤어리베치 꽃색 유전분리 결과로 볼 때 본 연구에서 사용된 품종들의 보라색, 분홍색, 그리고 백색 꽃은 서로 다른 두 쌍의 유전자 열성상위 작용에 의해 발현되며, 연분홍색은 분홍색에 대하여 단일 열성관계인 것으로 나타났다. 본 연구결과는 헤어리베치 품종 육성의 기초 자료로 유용하게 활용될 수 있을 것으로 기대된다. Flower color of hairy vetch (Vicia villosa Roth) was observed individually in F2 of crosses among violet, white, pink flower varieties, and between pale pink and pink flower varieties for providing information of useful gene as source of colored flower. F2 segregation mode of flower color between cross of white/violet was fitted to 3 violet : 1 white, and same segregation ratio of 3 violet : 1 pink was found in F2 of cross of violet/pink flower variety. Also, F2 segregation of flower color between cross of pale pink/pink flower variety was fitted to 3 violet : 1 white. When white flower was crossed with pink flower, flower color in F2 population segregated into 9 violet : 3 pink : 4 white. Based on the results, this study indicated that flower color of parental varieties was controlled by recessive epistatic interactions of two genes, and pale pink flower color was recessive to the pink color of the parental variety. This flower color inheritance may be used for practical application in breeding of hairy vetch variety.