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쌍금탕의 약효에 관한 연구 (제2보) : 급성위염 및 궤양에 대하여
한대석(Dae Suk Han),고건일(Kun Il Ko),김재백(Jae Baik Kim) 한국생약학회 1984 생약학회지 Vol.15 No.4
The effects of Ssangkum-tang on the acute gastritis and the gastric ulcer caused by acetic acid were studied. The water extract improved the inflammation of the acute gastritis and decreased the diameter of the ulcer area.
김형민(Hyung Min Kim),한상수(Sang Su Han),고건일(Kun Il Ko),손동환(Dong Hwan Son),전창덕(Chang Duk Jun),정헌택(Hun Taeg Chung),김재백(Jae Baek Kim) 대한약학회 1993 약학회지 Vol.37 No.5
Human insulin gene is consisted of the polymorphic region with the repeating units, the regulatory sequence, the structural gene including the intervening sequence, and 3''-flanking region. The polymerase chain reaction, which amplifies the target DNA between two specific primers, has been performed for the amplification of human insulin gene and simple one-step cloning of it into Escherichia coli. Out of 1727 nuceotides compared, only 4 sites were variable: 5''-regulatory region(G2101 -> AGG); IVS I(T2401 -> A); Exon II(C2411 deletion); IVS II(A2740 dejection). The variations at the G2101 and T2401 were the same as those found in one American allele. The other two variations were observed only in the specific Korean allele. And, the enzyme digestion patterns among normal, insulin dependent diabetes mellitus, and non-insulin dependent diabetes mellitus were the same. On the other hand, PCR method showed the possibility of the quickaccess for the polymorphic region in terms of the restriction fragment length of polymorphism.