집단급식용 생계육에서 분리된 Salmonella의 항생체 내성과 위생 처리제의 영향

강지현,이영덕,정기창,박종현,Kang, Ji-Hyun,Lee, Young-Duck,Jung, Ki-Chang,Park, Jong-Hyun 한국식품과학회 2001 한국식품과학회지 Vol.33 No.5

단체급식용으로 납품되는 생계육에서 Salmonella를 분리하여 생육제어를 위한 생리특성을 분석하였다. 계육의 총균수는 표피의 내부 살부위에서도 표피 균수의 약 10%정도가 검출되었으며 75 개 계육시료로부터 선택배지를 사용하여 분리한 후 최종적으로 51 주의 Salmonella를 동정하였다. 이들 균주 중 약 70%의 Salmonella는 4종 이상의 항생제에 내성을 보이는 다제내성균으로 보여 국내에서의 기 분리균에 비해서 높은 비율의 항생제에 대한 내성을 나타내었다. 이들 Salmonella에 대한 여러 가지 위생처리제인 유기산의 중 lactic acid와 trisodium phosphate를 agar disk diffusion으로 표준균주인 S. typhimurium ATCC 14028, S. typhimurium ATCC 12023, S. heidelberg and S. enteritidis(isolated from egg)와 비교하였다. Lactic acid는 분리된 Salmonella나 표준균주 모두 비슷한 정도의 내성을 보여 주었고 오히려 분리 Salmonella는 더 내성이 적었다. 그러나 최근에 중요시되는 trisodium phosphate에는 표준균주에 비하여 분리된 wild type에서 내성이 강한 균주가 거의 절반에 가까운 정도로 많음을 알 수가 있었다. 또한 trisodium phosphate에 대한 내성은 처리시간과 처리 후 재생배지에 따라 다르게 보여서 이들은 환경에 따 라 내성이 다르게 나타난다는 것을 알았다. 계육에 오염된 Salmonella는 wild type이므로 실제 위생처리제로 사용할 때는 좀더 철저한 처리가 요망되었다. 그러므로 집단 급식소에 납품되는 계육은 브로일러 사육단계에서의 항생제 관리와 도계과정으로부터 세균오염을 줄여야 하는 노력과 함께 이러한 식중독균의 제어를 위한 위생처리제에 대한 지속적인 기술개발도 필요할 것이다. To control the growth of Salmonella on raw chicken supplied in food service, they were isolated and analysed for their physiological characteristics. Total viable microbe counts under the skin of the chicken amounted to 10% of numbers on their skin. Fifty one of Salmonella were isolated and identified from 75 chicken carcass samples. About 70% of the isolates showed resistance to more than four antibiotics, which indicated very high resistance among the strains ever reported in Korea. Lactic acid and trisodium phosphate(TSP) as sanitizing agent were applied to the Salmonella spp. isolates by agar diffusion method. The resistance by isolates to those sanitizing agents was compared with type strains of S. typhimurium ATCC 14028, S. typhimurium ATCC 12023, S. heidelberg and S. enteritidis. The isolates showed similar or less resistance to the type strains as for the lactic acid. However, as for trisodium phosphate known recently as a good sanitizer, 50% of the isolates showed more resistant compared with the type strains. Also, the treatment time and regeneration medium affected significantly the resistance of the isolates, which indicates that more careful test might be needed for TSP's practical application to raw chicken. Therefore, From the results, it is suggested that antibiotic treatment during breeding broiler and contamination after slaughtering should be minimized along with careful use of sanitizing agent in order to ensure more safe raw chicken supply.

Novosphingobium pentaromtivorans US6-1에 의한 고분자 방향족 탄화수소 생분해과정에서 2-hydroxypropyl-$\beta$-cyclodextrin의 영향

강지현,권개경,김상진,Kang Ji-Hyun,Kwon Kae Kyoung,Kim Sang-Jin 한국해양환경·에너지학회 2004 한국해양환경·에너지학회지 Vol.7 No.3

포접력을 지닌 2-hydroxypropyl-β-cyclodextrin(β-HPCD)을 비롯한 cyclodextrin계 화합물은 소수성 유기물질의 용해도를 증가시킴으로써 미생물에 의한 분해를 촉진시키나 그 외의 자세한 기작은 알려져 있지 않다. 본 실험에서는 β-HPCD 유무에 따라 고분자 PAHs 분해력을 지닌 Novosphingobium pentaromtivorans US6-1 균주의 pyrene라 benzo[a]pyrene(B[a]P)의 분해정도, 이때의 biomass 변화 및 dioxygenase활성을 측정함으로써 PAHs 생분해과정에서 β-HPCD의 역할을 규명하고자 하였다. 실험구는 균주와 PAHs, β-HPCD의 존재 유무에 따라 8개 조건으로 준비하였으며 각 실험구의 배양기간에 따른 PAHs 분해도와 생체량의 변화를 측정하였다. Pyrene의 경우 β-HPCD가 존재함에 따라 분해도가 증진되는 것이 확인되었으며, 특히 B[a]P의 분해에는 β-HPCD가 필수요소로 작용하였다. 생체량의 변화는 PAHs의 존재 유무에 영향을 받지 않았고 β-HPCD의 존재 유무에 따라 차이를 나타내었다. 또한 US6-1 균주는 β-HPCD가 포함된 MM2 무기영양배지에서 전배양 할 때에 ZoBell 배지에서 전배양하는 경우에 비해 catechol-1,2-dioxygenase 효소활성이 높은 것으로 나타났으나 그 값은 빈영양상태에서 배양한 세포의 효소환성과 큰 차이를 보이지는 않았다. 이상의 결과로 볼 때 β-HPCD는 PAHs의 이용성을 높여주는 동시에 탄소원으로 이용되어 균주의 생체량 증가에 기여함으로써 PAMs의 분해력을 증진시키지만 dioxygenase 효소활성에는 영향을 미치지 않는 것으로 사료된다. Cyclodextrin compounds including 2-hydroxypropyl-β-cyclodextrin(β-HPCD) though to be accelerate the biodegradation of PAHs molecule by increasing solubility of PAHs through detaining PAHs in their's cavity. However, only this mechanism is not sufficient to explain the enhancement of PAHs biodegradation by β-HPCD. To find out possible additional role of β-HPCD in the enhancement of PAHs biodegradation, biodegradation rates of pyrene and benzo[a]pyrene (B[a]P) by a PAHs degrading Novosphingobium pentaromtivorans US6-1 strain were compared between with and without addition of β-HPCD. Changes of bacterial biomass were also measured simultaneously. In addition catechol 1,2-dioxygenase activity was determined depending on pre-incubation conditions. As a result, β-HPCD accelerate the degradation rate of pyrene by strain US6-1 and especially the β-HPCD amendment was obligatory for the degradation of B[a]p. Bacterial biomass was responsible for β-HPCD, however, PAHs compounds such as pyrene and B[a]P did not contribute to the bacterial biomass. Catechol 1,2-dioxygenase specific activity of US6-l cells pre-cultured in MM2 medium containing l% β-HPCD was higher than that of cells pre-cultured in ZoBell medium. The former case also showed similar activity compared to that of cells serially starved in MM2 medium after grown in ZoBell medium. These results imply that the presence of β-HPCD accelerate the degradation of PAHs by increasing the bacterial biomass as well as by increasing the water solubility of PAHs.

태권도장 서비스 공정성이 고객만족, 관계마케팅 및 고객충성도에 미치는 영향

강지현(Kang Ji-Hyun),전익기(Jeon Ik-Ki) 한국체육과학회 2008 한국체육과학회지 Vol.17 No.4

Many taekwondo gyms are randomly established. Therefore, the gyms need to put efforts to secure competitiveness. Facilities and training programs of the gyms are becoming focus on trainees and their parents. More efficient management strategy became necessary considering the taekwondo gym as a commercial sports facility. Thus, this research would suggest basic data to establish marketing strategy of taekwondo gym by defining relationships among service fairness, customer satisfaction, relations marketing, and customer loyalty and suggest efficient management strategy. Parents of taekwondo trainees in Seoul and Gyeonggi area were setup as population of survey subject and samples were extracted with convenience sampling which is a non-probability sampling method. 420 parents participated in the survey, but 402 answered questionnaire sheets excluding 18 sheets that were considered as insincerely answered or had some unanswered questions. The data was processed using SPSS/PC+ 12.0K Version for Windows and AMOS 5.0 statistics program for frequency analysis, exploratory factor analysis, confirmative factor analysis, reliability analysis, correlation analysis, structural equation model analysis, and got the following results. First, service fairness of taekwondo gym had significant effect on customer satisfaction. Second, service fairness of taekwondo gym had significant effect on relationship marketing. Third, service fairness of taekwondo gym had no significant effect on customer loyalty. Fourth, customer satisfaction of taekwondo gym had significant effect on relationship marketing. Fifth, relationship marketing of taekwondo gym had significant effect on customer loyalty.

지역사회 애착도가 경륜장에 대한 지역주민의 태도에 미치는 영향

강지현(Kang Ji-Hyun),양걸(Yang Girl) 한국체육과학회 2010 한국체육과학회지 Vol.19 No.3

The purpose of this study was to identify the effect of community attachment on attitude. The cycle race track that are located on Gwangmyeong area were set and 300 questionnaire were givens as the target of this research. The samples were collected through the convenience sampling method and a total of 243 copies were selected as final valid samples excepting 57 questionnaires which were judged to respond unfaithfully. As for data processing, this research made a frequency analysis, exploratory factor analysis (EFA), reliability test, correlation relative analysis and multiple regression were carried out using SPSS 17.0. As the result of analysis; first, community attachment had a positive influence on economic impact. second, community attachment had a positive influence on social-cultural impact. third, community attachment had a positive influence on environmental impact.

황표지에 있어 < 작자의 희화화 (戱畵化) > 취향의 전개사고 - 연천춘정 (戀川春町)과 붕성당희삼이 (朋誠堂三二)를 중심으로 -

강지현(Ji Hyun Kang) 한국일본어문학회 2000 日本語文學 Vol.9 No.-

N/A N/A

케이블TV 스포츠채널의 채널브랜드자산이 광고수용성에 미치는 영향

강지현(Kang, Ji-Hyun),양걸(Yang, Girl),송정명(Song, Jeong-Myeong),전익기(Jeon, Ik-Ki) 한국체육과학회 2012 한국체육과학회지 Vol.21 No.1

The purpose of this study is to provide broadcasters with basic data for managing their channel brand equities by suggesting the data about the relationship between channel brand equity and advertisement acceptance as well as the difference in receiver’s advertisement acceptance with regard to channel brand equity. The students of K, S and D universities located in Gyeonggi area, who are watching cable TV now, were selected as a population. For the study target sampling, among non-probability sampling methods, convenience sampling method was used, total 250 questionnaires were distributed and collected 227 questionnaires were used as final effective samples. For the data analysis, SPSS for window 17.0 statistic package was used to perform frequency analysis, reliance analysis, exploratory factor analysis, one-way ANOVA, correlation analysis and multiple regression analysis. Following results were drawn. First, from the result of verifying difference among broadcasters, it was found that channel loyalty, channel recognition and advertisement acceptance of SBS SPORTS were relatively higher than those of other broadcasters but for channel image, there was no significant difference in 3 broadcasters. Second, from the result of analyzing the correlation between channel brand equity and advertisement acceptance factors, the correlation between all factors excluding channel image was significant within significance level .01, showing mutually high correlation. Third, from the result of analyzing the effect of channel brand equity on advertisement acceptance, channel recognition gave a significant effect to 3 channels, channel loyalty gave a significant effect to 2 channels and channel image gave a significant effect to only 1 channel.

야구 동호회 참여에 따른 몰입경험이 여가만족에 미치는 영향

강지현(Kang Ji-Hyun),양걸(Yang Girl) 한국체육과학회 2011 한국체육과학회지 Vol.20 No.3

The purpose of this study was to investigate the effects of baseball club participants’ flow experience on leisure satisfaction. This study set the member of society baseball clubs registered with the National Sports Association baseball team who reside in Gyeonggi-do 4 area its survey targets, and selected 229 surveys as its final valid samples. According to SPSSWIN ver. 15.0, authors executed the exploratory factor analysis, reliability analysis and multiple regression. We obtained the following results: First, flow experience had a positive influence on relaxation satisfaction. Second, flow experience had a positive influence on educational satisfaction. Third, flow experience had a positive influence on psychological satisfaction. Fourth, flow experience had a positive influence on social satisfaction.

제품광고 모델로서의 스포츠스타 이미지 포지셔닝

강지현(Kang, Ji-Hyun),문영재(Mun, Young-Jae),전익기(Jeon, Ik-Ki) 한국체육과학회 2012 한국체육과학회지 Vol.21 No.6

This study aimed to identify how customers estimate sport star image by product advertising model and which brands are recognized as the same quality by using MDS(multi-dimensional scaling) understanding the position of graphs which is based on the image attribute. The survey samples were selected with convenience sampling among college students, who are gaining much attention as a new consumer group. Total 200 students were surveyed and among them, 182 surveys were collected and used in this study. The survey tool used was a questionnaire, and the sport stars were selected after presents from CM strategic studies institute CM Value and discussion with 5 experts to find the 6 sport stars(Kim Yu Na, Park Ji Sung, Son Yeon Jae, Park Chan Ho, Park Tae Hwan, Choo Sung Hoon,) who have relatively high awareness from the consumers. The reliability coefficient of the factors in image properties was shown to be a = 0.828. The ways of analysis are PROXCAL analysis technique and Multiple Regression Analysis of MDS. As a result of this study, Kim Yu Na and Son Yeon Jae are in competition and Park Chan Ho and Park Ji Sung are in competition, but Park Tae Hwan and Chao Sung Hoan don’t have competition relationship. Using the value of the property vector found in regression analysis, the results showed that Kim Yu Na was at the highest positioning in polish, beautiful, sexy, elegant and chann, and Park Ji Sung was positioned the highest in expert, trust, belief, credit, honesty, professional, serious, experience and skill. Also, Park Chan Ho had the highest positing in knowledge.

종교적 동기에 의한 의료행위의 형법적 고찰

강지현(Kang, Ji Hyun) 부산대학교 법학연구소 2016 법학연구 Vol.57 No.1

중요도-만족도(Importance-Performance) 분석을 활용한 국내 스포츠관광의 활성화 전략

강지현(Kang, Ji-Hyun),송정명(Song, Jeon-Myeong),전익기(Jeon, Ik-Ki) 한국체육과학회 2013 한국체육과학회지 Vol.22 No.3

The purpose of this study was to survey participation status of sports tourists activity and seek the way to revitalize tourist attraction by identifying the recognition of sports tourist attraction using IPA (Importance-Performance Analysis). The results were as follows. The quadrant I is “the concentrate here” part. And the quadrant I includes “travel time”, “ease of accommodation”, “ease of transportation”, “accessibility of transportation”, “Beautiful natural scenery”. The quadrant Ⅱ is “the keep up the good work” part. And the quadrant Ⅱ includes “Climate and natural conditions”, “appropriateness of the cost of the sport tourism”, “appropriateness of the cost of the other”, “appropriateness of transportation expenses”, “Scenery and atmosphere of sports tourist attraction” The quadrant Ⅲ is “the low priority” part. And the quadrant Ⅲ includes “Pageantry of Sports tourism activities”, “Kindness and hospitality of Residents”, “image of sports tourist attraction”, “infonnativeness about sports tourist attraction”, “usability of sports tourist attraction”. The quadrant Ⅳ is “the possible overkill” part. And the quadrant Ⅳ includes “service of employee”, “Specialties and tourism souvenir”, “entertainment and convenience of at night”, “food of abundant”.