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누에 전염성(傳染性) 연화병(軟化病)의 발병(發病) 억제제(抑制劑)에 의한 방제(防除)
강석권 ( Seok Kwon Kang ),김근영 ( Keun Young Kim ),이재창 ( Jae Chang Lee ),조용섭 ( Yong Sup Cho ) 한국잠사학회 1983 한국잠사곤충학회지 Vol.25 No.2
누에병 중에 피해가 막대한 전염성 연화병을 방제하기 위하여 바이러스 증식을 특이적으로 억제하는 guanidine유도체중에서 그 억제효과가 큰 guanidine hydrochloride(GH)를 도입하여 전염성 연화병 바이러스의 증식억제효과에 관하여 조사하였다. 1. 누에에 대한 GH의 약해시험 결과에서는 GH농도 0.01% 수준 이하에서는 약해가 전혀 없었다. 2. FV 감염잠 혼입에 따른 피해조사에서는 화용비율은 무처리(대조) 89.2% (100)에 대하여 1% 감염잠혼입구 70.7%(79), 5% 감염잠 혼입구 38.4%(43)이었으며 1만두수견양은 대조구 21.3kg(100)에 대하여 1% 혼입구 16.7kg(77), 5% 혼입구 8.8kg(41)으로서 전자와 같은 경향으로 나타났다. 3. FV에 대한 GH의 발병억제효과는 GH 0.01% 농도에서 대조구에 비하여 약 10배의 억제효과가 있었다. 4. GH의 투여회수와 발병억제효과에서는 FV접종 후 계속 투여하는 것이 가장 효과적이었다. 5. FV 접종 후 GH투여시간에 대한 발병억제 효과는 접종과 동시에 투여는 것이 가장 효과적이었다. 6. FV의 GH에 의한 시험관내 불활화 조사에서는 GH가 시험관내에서 FV를 불활화 시키는 작용이 인정되지 않았다. The prevalence of the infectious flacherie virus (FV) disease causes a severe damage to cocoon yield and various methods to control the disease have been studied. In this regard, guanidine hydrochloride (GH), one of the guanidine derivatives known as the most inhibitory agent against the replication of picorna virus, was applied to silkworms per os with mulberry leaves and the results were as follows. 1. The application of GH below 0.01% of the chemical concentration did not give any damage to silkworm larvae. 2. The transmission of the virus disease by introducing the FV infected larvae to the healthy larvae group was proportioned to the number of infected larvae. When l% of infected larvae was introduced to the rearing tray of healthy larvae, the pupation rate was 70.7% (79) and it was 38.4% (43) to 5% of infected larvae introduced, while the control of non-mixed with infected larvae gave 89.2% (100) of pupation rate. The cocoon yield from 10,000 larvae also showed the same tendency as the pupation rate. 3. The inhibitory effect of GH against the replication of FV showed ten times in treatment of 0.01% of the chemical agent compared to the non-treatment. 4. The successive application of GH after virus inoculation to silkworm larvae led to the most effective on the inhibition of the virus replication. 5. The immediate application of GH after the virus inoculation also gave the best effect on the inhibition of the virus replication in silkworm larvae. 6. The effect of GH on the inactivation of FV in vitro was not observed.
솔잎 혹파리의 병원미생물(病原微生物)에 관(關)한 조사연구(調査硏究) -특(特)히 가잠경화병(家蠶硬化病)과 관련하여-
강석권 ( Seok Kwon Kang ),조용섭 ( Yong Sup Cho ),박호용 ( Ho Yong Park ),고성철 ( Seong Chul Ko ) 한국잠사학회 1982 한국잠사곤충학회지 Vol.24 No.1
본 시험은 솔잎혹파리의 미생물학적 방제대책의 일환으로서 미생물의 분리동정과 병원성을 연구 조사하고자 행하여졌다. 1. 솔잎혹파리의 유충에서 분리동정 된 균은 Verticillium psalliotae, Aureobdsidium pullulansa, Streptomyces sp., Aspergillus sp.(2종), Ascochyt, sp.의 6종이었으며 6종 전부가 병원성이 있었다. 2. 추기 양잠농가로부터 Spicaria pracina, Isaria farinosa, Oospora destructor, Aspergillus oryzae, A. flavus의 5종의 누에 경화병균을 분리 동정하여, 솔잎혹파리유충에 대한 병원성을 검정한 결과, 5종 전부가 강력한 병원성을 나타내었다. 3. 솔잎혹파리 유충에서는 29종의 colony가 나타났으며 그 중에서 Bacillus 계통이 11, coccus 계통이 9개로 구분되었으며, 현재 병원성검정은 진행 중에 있으나 No.4와 No.9-1균주는 병원성이 인정되었다. The study has been carried out to investigate a possibility to control the pine needle gall midge, Thecodiplosis japonensis Uchida et Inoue, by microbial pathogens as one of the microbial control measures. The samples were collected at Kocheon-Ri in the suburbs of Suweon city where were heavily damaged by this pest. Microorganisms were isolated from the both of diseased larvae and baiting method of soil microbes. In addition to, several species of the silkworm mucardine diseases were isolated for their infectivity of these fungi to the larvae of pine needle gall midge. Six species of fungi, Aureobasidium pullulans, Ascochyta sp., Verticillium psalliotae, Streptomyces sp., and two species of Aspergillus were isolated from the galls and soils, five species of muscardine diseases, Isaria farinosa, Spicaria pracina, Oospora destructor, Aspergillus flavus and A. oryzae were also identified from the silkworm corpse collected in the silkworm rearing farmers. Total of eleven species of fungi identified from the both of the larval of pine needle gall midge and silkworm larvae were tested for their pathogenecity to the larvae of pine needle gall midge. All of eleven species of fungi identified showed a considerable infectivity to the larvae. Twenty nine different kinds of bacteria were isolated from the both of diseased larvae and infested soils through the direct planting for the larvae and streaking for the corpse. The infectivity test is in progress. However two kinds of bacteria were recognized to be pathogenic to the larvae tested.
가잠(家蠶)의 연화병(軟化病) 바이러스에 관(關)한 연구(硏究) -연화병(軟化病) 바이러스 Polypeptide의 성상(性狀)-
강석권 ( Seok Kwon Kang ),김근영 ( Keun Young Kim ) 한국잠사학회 1979 한국잠사곤충학회지 Vol.21 No.2
1. 연화병 바이러스의 단백질을 SDS-polyacrylamide gel 전기영동으로 분석한 결과 분자량 37,500(FP I), 30,500(FP II) 및 26,500(FP III)에 상당하는 3종류의 polypeptide가 얻어졌으며, 이들의 비율은 FP I이 6.6%, FP II가 25.0% 및 FP III가 68.4%였다. 2. 연화병 바이러스의 단백질을 amino산 분석을 행한 결과, 17종의 amino산이 분리되었으며, 이 amino산 조성은 타곤충 바이러스의 amino산 조성과 유사하였다. Purified preparations of flacherie virus capsid protein were fractionated by SDS-polyacrylamide gel electrophoresis, and amino acid composition was determined by amino acid analyzer. Three polypeptide components, FP I, FP II and FP III were detected, and the molecular weights of these components were 37,500, 30,500 and 26,500 respectively. The FPIII was major poly-peptide comprised about 68.4% of the total virus capsid protein. Seventeen amino acids were detected by an amino acid analyzer from hydrolyzate of the virus capsid protein and the pattern of amino acid composition was similar to those of several other insect viruses.
가잠(家蠶)의 연화병(軟化病)바이러스에 관(關)한 연구(硏究) -연화병(軟化病)바이러스의 정제(精製)-
강석권 ( Seok Kwon Kang ) 한국잠사학회 1977 한국잠사곤충학회지 Vol.19 No.1
1. 종내의 보고된 FV의 체제법을 비교검토한 결과, 어느 방법도 정제도 및 정제효율에 문제가 있음을 지적하였다. 2. 종래의 정제방법을 개량하여 정제도·정제효율이 뛰어난 FV의 정제법을 확립하였다. 3. 새로운 정제법을 이나주바이러스의 정제에 적용한 결과, 본 바이러스의 정제에도 유효함이 구명되었으며 동시에 최초로 본 바이러스를 정제하는데 성공하였다. 4. 새로운 정제법으로 정제한 FV 및 이나주바이러스 입자를 전자현미경으로 관찰한 결과, 이들 바이러스입자는 구형입자이고, 그 직경은 전자는 27nm, 후자는 20nm이었다. 또 이나주바이러스 입자의 침강정수는 80S였다. The flacherie (FV) and Ina-flacherie (Ina-FV, named arbitarily) viruses of the silkworm, Bombyx mori was isolated from infectious larvae. Two types of infectious particles were purified by sucrose density gradient centrifugation. Some properties of the purified particles were investigated. Electron micrographs showed that FV and Ina-FV were spherical particles with diameters of 27nm and 20nm, respectively. The sedimentation coefficient of Ina-FV was 80S.
배추흰나비 과립병(顆粒病)바이러스 감염조직(感染組職)에서의 바이러스 성숙(成熟)
유강선,강석권,Ryu, Kang-Sun,Kang, Seok-Kwon 한국현미경학회 1991 Applied microscopy Vol.21 No.1
The virus maturation in fat body cells infected with P. rapae granulosis virus were also examined to have the following results. Thin section of P. rapae GV granules showed a regular lattice of the granule enclosing virus particles. Virus particles were observed to penetrate the fat body cells by fusion or viropexis type. The blood and fat body cells uptaked the granulosis viruses a phagosome, in which they were digested by lysosomal enzymes.
김동희 ( Dong Hee Kim ),강석권 ( Seok Kwon Kang ),김도훈 ( Do Hoon Kim ),이의철 ( Eui Chul Lee ) 대한골절학회 2013 대한골절학회지 Vol.26 No.1
Compared with acromioclavicular dislocation, dislocation of the clavicle at its sternal end is uncommon and accounts for 3% of all injuries to the shoulder girdle. Furthermore, the posterior dislocation of the sternoclavicular joint is relatively a rare injury compared to the other types of sternoclavicular dislocation. We report this case since we have experience with similar cases of traumatic posterior dislocation at the sternoclavicular joint, which were successfully treated with x-ray guided reduction.
새로운 Baculovirus 전이벡터를 이용한 Escherichia coli ${\beta}$-galactosidase 유전자의 발현
우수동,김우진,김혜성,진병래,강석권,Woo, Soo-Dong,Kim, Woo-Jin,Kim, Hye-Seong,Jin, Byung-Rae,Kang, Seok-Kwon 한국미생물 · 생명공학회 1996 한국미생물·생명공학회지 Vol.24 No.1
To investigate the expression efficiency of new transfer vector of Bombyx mori nuclear polyhedrosis virus (BmNPV), Escherichia coli lacZ gene was inserted into new transfer vector pBmKSK1, under the control of polyhedrin promoter and expressed in BmN-4 cells and larvae of silkworm, Bombyx mori. The recombinant virus containing lacZ gene was isolated from BmN-4 cells coinfected with transfer vectro pBmKSK1-LacZ and wild type BmNPV genome, and analysed by Southern blotting. The expression of ${\beta}$-galactosidase was characterized by SDS-PAGE, Western blotting and ${\beta}$-galactosidase activity assay. The results showed that the level of expression in silkworm larvae was higher than that of BmN-4 cells.