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      • KCI등재

        태아의 간과 흉선 조직 그리고 조혈모 세포를 이식한 Rag2<SUP>-/-</SUP>γc<SUP>-/-</SUP> Mice에 사람의 면역 세포 형성

        강미진(Mijin Kang),주성연(Sung-Yeon Joo),최봉금(Bong-Kum Choi),정다연(Da-Yeon Jung),최호인(Ho-In Choi),박재범(Jae Berm Park),최규성(Gyuseong Choi),권준혁(Choon Hyuck Kwon),김성주(Sung-Joo Kim),조재원(Jae-Won Joh) 대한외과학회 2008 Annals of Surgical Treatment and Research(ASRT) Vol.74 No.1

        Purpose: Many researchers have tried to develop animal models that mimic the human immune system, e.g. a humanized mouse model, to improve the engraftment of hematopoietic stem cells and develop human immune cells in an animal model. This study evaluated the feasibility of the cultured human umbilical cord blood (hUCB)-derived CD34? cells for cell expansion, in Rag2<SUP>-/-</SUP>γc<SUP>-/-</SUP> mice, and establish co-transplantation with human fetal thymus/liver tissue (Thy/Liv) under the kidney capsule. Methods: Co-transplantation of hUCB-derived CD34? cells with Thy/Liv was performed. The hUCB-derived CD34? cells were prepared by freshly thawing (G1) and culturing for 7 days with two types of cytokine combinations (G2, G3). The CD45? cell populations were measured at 6, 8, 10 and 16 weeks in the peripheral blood. The splenocytes were cultured with mitogenic stimuli (PHA -L or IL-2) at 20 weeks posttransplantation, and the proliferation of human immune cells was evaluated. Results: There were no significant differences in the human CD45? cell populations at 6, 8, 10 and 16 weeks post-transplantation between the groups. In the cultured splenocytes at 20 weeks post-transplant with PHA-L or IL-2, there was remarkable expansion of CD3? cells in the three groups. Although no CD19? cells were detected in the spleen, human Ig G was detected in the sera of these mice. Conclusion: The cultured and expanded hUCB-derived cells with cytokine combinations might be a feasible cell source in humanized mouse modeling. In addition, human immune cells can be reconstituted from the co-transplantation of Thy/Liv and cultured hUCB-derived CD34? cells.

      • KCI등재후보

        중국 부부 문제의 현황, 원인 및 과제

        조양(Yang Zhao),강미진(MiJin Kang) 사단법인 한국부부가족상담협회 2023 부부가족상담연구 Vol.4 No.2

        본 연구의 목적은 중국의 부부 관계에서 나타나는 문제점들을 살펴보고, 그 원인과 과제를 확인하는 것이다. 이를 위해 중국에서 나타나는 사회 현상들과 결혼에 관련한 문헌, 자료를 분석하였다. 그 결과 사회주의의 개방으로 인한 빠른 경제 성장과 서구의 가치관의 유입으로 가족의 구조가 대가족에서 핵가족으로 변화되었으며, 전통 가치관과 서구적 사고의 갈등, 높은 이혼율과 늦은 나이의 결혼, 저출산, 농촌과 도시의 빈부 격차 등의 문제가 있는 것으로 확인되었다, 또한 현대화로 인한 핵가족화, 결혼에 대한 가치관의 변화, 여성의 사회진출 확산, 높은 이혼율과 낮은 출산율 등이 중국 부부 관계에 존재하는 일반적인 문제로 확인되었다. 부부갈등의 원인으로는 경제적, 감정적, 가치관의 차이, 고부간의 관계에서 오는 갈등 등이 주된 원인으로 확인되었다. 본 연구결과를 바탕으로 중국 부부의 문제 해결을 위한 과제를 논의하였고 끝으로 후속 연구에 대한 제언과 시사점을 제시하였다. The purpose of this study is to examine the problems that arise in marital relations in China and identify their causes and potential solutions. To achieve this, Chinese literature and statistical data related to marriage were analyzed. As a result, it was confirmed that there are issues such as conflicts with traditional values, high divorce rates, delayed marriages, and low birth rates due to rapid economic growth and the influence of Western values. Additionally, nuclear family structures resulting from modernization, changing marriage values, the increasing involvement of women in society, and high divorce rates and low birth rates have been identified as common problems in China. The main causes of marital conflicts were identified as economic disparities, emotional differences, value conflicts, and tensions in the relationships between mothers-in-law and wives. Based on the findings of this study, solutions to address the problems faced by Chinese couples were discussed, and finally, suggestions and implications for future research were presented.

      • KCI우수등재

        국가 화학물질 유해성정보 데이터베이스 구축 과정의 신뢰도 제고 방안에 관한 연구

        이소민(Somin Lee),이민혁(Minhyeok Lee),강미진(Mijin Kang),권순광(Soon-Kwang Kwon),나진성(Jin-Sung Ra),박백수(Beaksoo Park) 한국환경보건학회 2020 한국환경보건학회지 Vol.46 No.4

        Objectives: According to the Act on Registration, Evaluation, Etc. of Chemicals, new and existing chemicals must be registered by 2030. In addition, industries need to submit hazard data as an attachment during the registration process. Therefore, we constructed a nationwide chemical database to support small industry by providing hazard data and original sources. During the process, we developed a new standard procedure for minimizing errors and increasing reliability. Methods: We analyzed the categories of errors and the cause of the errors through the verification results of the 2019 project. We present an improved database construction methodology and system. Results: Errors are categorized according to their causative factors into simple, technical, and structural type errors. Simple errors arise simply because of decreased concentration or negligence in following the instructions. Technical errors are caused by a discrepancy between the professional field and the type of data. Structural errors indicate systemic errors such as incomplete forms on the excel database or ambiguity in the guidelines. Lessons from the errors collected in the 2019 project are used to update the procedures for database authorization and technical guidelines. The main update points are as follows; ‘supplementation of review process’, ‘giving regular training to external reviewers’, ‘giving additional information to authors, like physico-chemical properties of substances, degradability, etc.’, ‘amendment of excel form’, and ‘guideline upgrades’. Conclusions: We conducted this study with the aim of improving the accuracy and reliability of the database of hazard information for chemical substances. The new procedures and guidelines are now being used in the 2020 project for construction of a hazard information database for Korea.

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