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난구세포와의 공배양이 돼지 체외수정란의 초기발달에 미치는 영향
한만희,임정훈,박병권,이규승 충남대학교 농업과학연구소 1996 농업과학연구 Vol.23 No.2
This study was conducted to investigate the effects of coculture for the development rate to morula/blastocyst stages of early porcine embryos, derived from oocytes matured and fertilized in vitro, with porcine cumulus cell monolayers(PCM) in the two different media, respectively. The rates of embryos developed to 2-, 4-, 8~16-cell and morula/blastocyst stage were 48.7, 40.5, 34.8 and 17.0% in Ham's F-10 with PCM, and 48.3, 35.6, 22.1, and 13.4% in TCM-HEPES with PCM, respectively. The above development rates to morula/blastocyst stages were significantly higher than those of the embryos cultured in the Ham's F-10 and TCN-HEPES without PCM(P<0.05). The in vitro development rates to the morula/blastocyst stage of 1-cell embryos cultured in Ham's F-10 and TCM-HEPES without PCM were 0.0~1.0%. Especially, most of embryos were observed to arrest the development beyond 4-cell stages. As shown in the above results, the coculture in vitro fertilized porcine embryos with PCM in the two different media enhanced the development of fertilized eggs to morula/blastocyst stages in vitro. However, we didn't find out any difference for the in vitro development to morula/blastocyst stages between Ham's F-10 and TCM-HEPES media.
배양액 및 난구세포가 돼지난포란의 체외수정에 미치는 영향
박병권,한만희,서길웅,박창식,이규승 충남대학교 농업과학연구소 1996 농업과학연구 Vol.23 No.2
This study was conducted to investigate the effects of medium and cumulus cell on in vitro fertilization of porcine follicular oocytes. The results obtained are as follows ; 1. The normal fertilization rates of in vitro matured follicular oocytes coultured in BO, mTALP and TCM-HEPES medium were 14.0~24.3%, 30.8~32.7% and 21.4~23.9%, respectively. These data indicated that the optimal medium for fertilization on porcine oocytes in vitro was the mTALP medium. 2. The normal fertilization rates of epididymal sperm were 24.3%(BO), 30.8%(mTALP) and 23.9%(TCM-HEPES), and those of ejaculated sperm were 14.0%(BO), 32.7%(mTALP) and 21.4%(TCM-HEPES). 3. The sperm penetration rates of cumulus-enclosed and cumulus-free oocytes on in vitro feritilization were 54.0% and 72.0%. The normal fertilization rates of cumulus-enclosed and cumulus-free oocytes were 11.9% and 21.5%. The normal fertilization rate of cumulus-enclosed oocytes was significantly(P<0.05) higher than that of cumulus-free oocytes.