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Toxoplasma gondii의 組機培養에 關한 硏究
金鍾冕 全北大學校 1974 論文集 Vol.16 No.-
Quite a number of diagnostic methods have been studied as to earlier diagnosis, treatment and control of toxoplasmosis where higher rates of occurance and mortality are discerned no less than in cases such as swine cholera and swine erysipelas. Even with the method of dyetest which is universely acknowledged as an efficient serological diagnostic method, however, chances are very slim for the treatment of swine suffering from acute toxoplasmosis because the antibody comes into being around seven days after the symptom. Hence, the major concern of the paper is with the study of the diagnostic method which has proved to be more efficient and valid. The buffy coat cell of three-day cultivation was inoculated with Toxoplasma gondii collected from the circulatory blood of the patients with parasitemia experimentally inoculated right after the symptom. The results obtained therefrom are summarized in the following outline : 1. The buffy coat cell of two-day cultivation was inoculated with buffy layer of the circulatory blood of swine and mouse experimentally infected with Toxoplasma godii. This experiment did not reveal the multiplication which is valuable for the diagnostic method. 2. The buffy coat cell of three-day cultivation was inoculated with buffy layer of the circulatory blood of swine with parasitemia experimentally inoculated. Then started to appear Toxoplsma gondii 24 hours after the inoculation, and the multiplication for valuable diagnosis was observable during the period of 48-72 hours post inoculation. 3. The buffy coat cell of three-day cultivaton was inoculated with buffy layer of the circulatory blood of mouse with parasitemia experimentally inoculated with Toxoplasma gondii, this experiment ending in the same result as in the case of swine.
김종면,김종연,장선일,최상훈,박관하,조정곤,최민순 한국어병학회 1995 한국어병학회지 Vol.8 No.2
파래투여에 따른 생리활성에 미치는 영향을 조사하기 위하여 이스라엘잉어에 대상으로 공기노출에 따른 혈액성상의 변화를 조사하였고 아울러서 비단잉어를 대상으로 A. sobriae에 대한 항균력과 항응집소가를 조사하였던 바 그 결과는 다음과 같다. 이스라엘잉어를 대상으로 4개월간 파래첨가사육후에 공기노출에 따른 혈액성상에 미치는 영향에 있어서 Ht치는 파래첨가의 유무에 관계없이 큰 차이를 보이지 않았으며, 헤모글로빈, 총단백, 알부민 및 글루코스치는 대조군에 비해 첨가군에서 증가되는 경향을 보였으며, 특히 5% 첨가군에서 높게 나타났다. 그렇지만, GPT 및 GOT치는 대조군에 비해 파래 첨가농도에 비례하여 감소하는 경향을 보였다. 한편, 비단잉어를 대상으로 파래추출물을 1주간 복강내로 투여후 A. sobriae에 대한 항균능은 대조군에 비해서 첨가군 모두에서 낮게 나타났으며, 특히 30㎍/g 투여군에서 4×104 CFU로 최저치를 나타냇다. 한편, 항응집소가는 대조군에 비해 파래추출물 투여군 모두에서 증가되었으며, 특히 30㎍/g 투여군에서 6.0±1.1로서 최고치를 보였다. Physiological activities of E. compressa were examined after oral and intraperitoneal(i.p.) adminstration in young(8g) Israeli and colored carp. Hematological parameters were evaluated to test physiological respose. Anti-bacterial activity was examined by counting the number of bacterial cells in the kidney, and also by measuring the change of agglutinin titers following A. sobriae infection. There was a tendency of increase in E. compressa-fed groups in total protein, albumin and glucose levels. The most marked increase was noted in the group fed with 5% E. compressa. GPT and GOT levels were reduced with the increase of E. compressa concentration. Feeding of E. compressa did not alter haematocrit(Ht) and hemoglobin (Hb) values. The number of A. sobriae was reduced in all groups intraperitoneally treated with variable concentration of E. compressa-extract. The lowest bacterial cells were found at the group intraperitoneally treated with 30㎍ of E. compressa-extract per g of colored carp(30㎍/g), indicating that the anti-bacterial activity is maximized at this concentration. The agglutinin titers were elevated in E. compressa extract-treated groups(30㎍/g) with the maximum value of 6.0±1.1. These results indicate that E. compressa adminstration activated physiological response, and triggered a cascade for anti-bacterial reaction.
김종면,Kim Jong Myon 대한수의사회 1963 대한수의사회지 Vol.7 No.5
Routine hematological exermination was made on 30 Korean cattles which are composed entirely of female in order to establish the normal blood values. The results obtained in the experimenet are summarized as follows. Class, Mean and standard deviation. Er
金鍾冕,朴營洙,宋憙鍾 대한보건협회 1980 대한보건연구 Vol.6 No.2
In the mixed culture of E. coli strain―L 129, L 136, L 139, L144, L145, and G 1253 and E.coli―ML 1410, Cp^r, Ap^2, Tm^r, and Sm^2 gene is thought to be transferred from the former to the latter by conjugation. The lactobacillus-cultural filtrates―L. acidophilus FM 4, L. casei FM 1, and L, lactis FM 3―exhibited a considerable suppressive effect on the R plasmids transmission from the 6 strains of clinical isolates of E. coli to ML 1410. In this experiment, we could observed the suppressive mechanisms of the plasmid gene transmission is not elimination or loss of the transfer gene but inhibition or blocking up of the mating system.