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김영호,YiSeul Kim,김영호 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.2
The fruit fly, Drosophila melanogaster, is mainly found in fermented and rotten fruits and is more tolerant to chemicals emitted during the fermentation process than other organisms. Its distinctive habitat suggests an evolutionary adaptation to the chemicals, such as acetic acid, ethanol, and 2-phenylethanol. Quantitative realtime PCR (qRT-PCR) assays can be performed to analyze the expression patterns of D. melanogaster genes putatively involved in chemical detoxification and metabolism, to better understand D. melanogaster adaptions to its environment. The selection of stably-expressed, internal reference genes across samples is crucial for accurate normalization of target gene expression parameters. In this study, we investigated the transcription levels of ten candidate reference genes: hsp22, ef1β, α-tub, rpL18, rpS3, argk, nd, tbp, gapdh, and ace, in D. melanogaster exposed to various concentrations of acetic acid, ethanol, and 2-phenylethanol, and expression stability of these genes was evaluated using three different programs: geNorm, NormFinder, and BestKeeper. These three software resulted in different stable genes, but suggested the selection of multiple reference genes for target gene normalization. For validation of reference genes, expression levels of adh were normalized with different references and combination of multiple genes. In the flies exposed to three chemicals, rpL18 was commonly suggested to be used for the most stable reference gene for comparing expression levels of genes potentially associated with environmental chemical tolerance in D. melanogaster.
김영호,김이슬,김동흔,김세연,서경진,신수현,이재영,김영호 한국곤충학회 2019 Entomological Research Vol.49 No.6
Drosophila melanogaster is attracted to chemicals produced by fermentation and it is abundantly found in rotten fruits. Considering its habitat, the fruit fly is reported to be tolerant to environmental chemicals. Quantitative real-time polymerase chain reaction was employed to investigate the expression pattern and physiological function of genes putatively involved in chemical detoxification. In quantitative real-time polymerase chain reaction assays, normalization of target gene expression with internal reference genes is required. These reference genes should be stably expressed during chemical exposure and in chemical-free conditions. In this study, therefore, we used two programs (geNorm and BestKeeper) to evaluate the expression stability of five reference genes (nd, rpL18, ef1β, hsp22 and tbp) in female adult flies exposed to various concentrations of methanol and ethyl acetate. Four genes (nd, rpL18, ef1β and tbp) were found to be suitable for use as reference genes in methanol-treated flies and three genes (ef1β, nd, tbp) were found to be suitable for use as reference genes in ethyl acetate-treated flies. These results suggested that a combination of two genes among these stably expressed genes can be used for accurate normalization of target gene expression in quantitative real-time polymerase chain reaction-based determination of gene expression profiles in D. melanogaster treated with both chemicals.
세미나 - 일본인쇄산업연합회 Gravure인쇄 Service(연포장) Green 기준 Guide Line
김영호 (사)한국포장협회 2011 包裝界 Vol.214 No.-
한국포장기술인협의회는 지난 해 10월 22일 서울 팔래스호텔 로얄볼룸에서 제26회 한국포장기술인 세미나를 개최했다. 이날 세미나는 '라미네이트용 수성접착제 기술과 적용 방법 및 일본인쇄산업연합회의 Gravure인쇄 Service(연포장) Green 기준 가이드 라인'을 주제로 진행됐다. 본 원고는 세미나에서 김영호 한국포장기술인협의회 회장이 발제한 자료로, 지난 1월호에 이어 인쇄 Service Green 기준과 Green Printing 인정제도, Gravure 인쇄 Service(연포장) Green 기준 및 해설에 대해 살펴보도록 한다.