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- 저자 : 金哲永 (검색결과 569 건)
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누에에 대한 低毒性 Bacillus thuringiensis 菌株의 選拔
金哲永,金英勳,姜錫權 한국잠사학회 1986 한국잠사곤충학회지 Vol.28 No.1
Among many microbial pesticides, Bacillus thuringiensis is one of the most hopeful pesticide and some commercial products have been appearing on the market. Because these commercial products contain living spores and toxins of the organism, there is a danger that living spores of B. thuringiensis may be scattered by wind and cause a great damage in the sericulture areas. In order to avoide these risks it is desirable to select the strain which has low pathogenicity to the silkworm, and at the sametime being highly pathogenic to the pest insects. Thus this study has been carried out to acquire some basic informations about the procedure of desirable strain selection. Three strains of B.thuringiensis var. kurstaki, var. dendrolimus and var. aizawai were used for the pathogenicity test on the silkworm, Bombyx mori and the fall webwarm, Iiyphantria cunea. Those strains were investigated by the agarose gel electrophoresis patterns of Plasmid DNA determine whether mutation had occured. Pathogenicity tests were carried out of using isolated crystal proteins and spore-crystal protein to mixtures of each strain, seperatively. In case of using spore-crystal protein mixture, the order of pathogenicity in varities of B. thuringiensis against B. mori and H. cunea were kurstaki, aizawai, dendrolimus and kurstaki, dendrolimus, aizawai, respectively. But using isolated crystal proteins, dendrolimus had the highest toxicity to H. cunea and the lowest toxicity to B. mori among tested three strains. From the above results, dendrolimus was presumed the most desirable strain for using microbial pesticide.
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김철영,구동억,김대호,홍현숙,이혜경,최득린,양승부,문철 대한영상의학회 2002 대한영상의학회지 Vol.46 No.3
Purpose: To analyse the effectiveness of percutaneous treatment of central venous obstruction in patients undergoing hemodialysis. Materials and Methods: In 100 patients, 107 central venous strictures [56 subclavian (occlusion:21, stenosis:35) and 51 innominate (occlusion:23, stenosis:28)] were assessed, and 170 percutaneous angioplasty procedures were performed. Balloon dilation of the venous lumen was the preferred mode, but if dilation was incomplete we inserted a stent at the site of the stricture. Technical success, procedural complications and the long-term patency rate were evaluated, and the patency difference according to location and degree of stricture, the existence of DM, and any history of central catheter insertion were also determined. Results: We inserted 52 stents in 170 procedures, in 157 (92.4%) of which initial technical success was achieved. Stent migration occurred in two cases and balloon rupture in three. The 6- and 12-month primary patency rates were 46.2% and 24.1%, respectively, and the mean patency rate was 8.5 months. The 1-, 2- and 3-year accumulative patency rates were 59.8%, 47.5% and 35.7%, respectively, and the mean patency rate was 23.5 months. Other than in the history of central catheter insertion, there were no statistically significant differences in patency rates (p=0.0128). Conclusion: In hemodialysis patients with a central venous stricture, percutaneous angioplasty is a safe and useful procedure, but to maintain long-term central venous patency, repeated interventions are required. 목적: 혈액투석 환자에서 발생한 중심정맥 협착에 대한 경피적 치료의 유용성에 대하여 알아보고자 한다. 대상과 방법: 중심정맥 협착을 가진 100명 107정맥을 대상으로 하였으며 쇄골하정맥 협착은 56명(폐색:21,협착:35), 무명정맥 협착은 51명(폐색:23,협착:28)이었고, 총 170회 경피적 확장술을 시행하였다. 풍선확장술을 먼저 시행하였고 충분한 내경을 확보할 수 없는 경우에는 stent를 삽입하였다. 기술적 성공율, 합병증 및 장기 개통율을 구하였으며 협착의 위치, 완전 폐색 여부, 당뇨 유무, 중심관 삽입력 여부에 따른 개통율에 차이가 있는지를 조사하였다. 결과: 170회 시술 중 52회에서 stent를 삽입하였다. 157회(92.4%)에서 기술적으로 성공하였고 2예에서 stent 이탈, 3예의 풍선파열이 발생하였다. 6, 12개월 1차 개통율은 각각 46.2, 24.1%이며 8.5개월의 평균 개통율을 보였다. 1년, 2년, 3년 누적 개통율은 각각 59.8, 47.5, 35.7%이며 평균 23.5개월의 개통율을 보였다. 중심관 삽입력(p=0.0128)을 제외하고는 개통율에 통계적 의의는 없었다. 결론: 혈액투석환자의 중심정맥 협착에 대한 경피적 치료는 안전하고 유용하지만 장기적인 개통성을 유지하기 위해서는 반복적인 시술이 필요하다.
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김철영,진윤태,윤익,김주영,박선민,정록선,김용식,이홍식,전훈재,이상우,엄순호,최재현,김창덕,류호상,현진해 대한소화기내시경학회 2003 Clinical Endoscopy Vol.26 No.6
Adenomatous polyposis coli (APC) is a rare autosomal dominant disorder in which about 20∼30% of affected individuals do not have a family history. It is characterized by hundreds to thousands of adenomas in the colon. APC usually develops during the second or third decade of life. If the polyposis is not treated surgically, colorectal cancer can develop in almost all patients before age 40. We experienced a case of adenomatous polyposis coli combined with rectal cancer in a 26-year-old male patient complaining of low abdominal pain without a family history of APC. (Korean J Gastrointest Endosc 2003;26:443-447) 26세의 남자가 하복부 통증을 주소로 내원하여 시행한 전대장 내시경에서 직장암이 동반된 가족력 없는 산발성 선종성 용종증을 진단받고 전직장-결장 절제술 및 영구적 회장조루술을 시행받았다. 선종성 용종증은 적어도 100개 이상의 선종성 용종이 대장에 산재하여 발생하고, 상염색체 우성으로 유전되는 드문 질환으로, 20~30%의 환자는 가족력 없이 APC 유전자(adenomatous polyposis coli gene)의 자발적인 돌연변이에 의해 발생하는 것으로 알려져 있다. 뚜렷한 가족력이 없이 발생하는 산발성 선종성 용종증은 드물게 발생하며 국내에서는 소아에서 보고된 1예만이 있으나, 성인에서 가족력 없이 진행성 직장암이 동반된 선종성 용종증의 보고는 없다. 또한 본 증례는 가족력 조사에 의존한 기존의 증례와 달리 가족력 조사뿐 아니라 직계 가족 전체를 대상으로 대장내시경을 시행하여 가족력이 없이 산발적으로 선종성 용종증이 발생한 것을 확인하였다. 따라서 저자들은 하복부 통증을 주소로 내원한 26세의 남자에서 가족력 없는 산발성 선종성 용종증과 함께 직장암이 병발하였던 1예를 경험하였기에 보고한다.
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김철영,강시현,정기화,정춘식,김박광,허훈,Kim, Chul-Young,Kang, Si-Hyun,Jeong, Gi-Wha,Cheong, Chun-Sik,Kim, Bak-Kwang,Huh, Hoon 한국생약학회 2000 생약학회지 Vol.31 No.1
In order to find out hypoglycemic constituents from natural resources, Korean medicinal plants being used for the treatments of diabetes mellitus were tested with streptozotocin-induced diabetic rats. From the above screening, the $CH_2Cl_2$ fraction of 80% methanol extracts of Morus alba leaves showed hypoglycemic activity. The $CH_2Cl_2$fraction was chromatographed on silica gel, resulting in 9 subfractions, and tested hypoglycemic activity. The subfraction which had a strong hypoglycemic activity was rechromatographed and two galactolipids (compounds 1 and 2) were isolated along with a triterpene (compound 3) from other fractions. On the basis of spectroscopic data, acid hydrolysis, alkaline hydrolysis and enzyme hydrolysis results, compounds 1, 2 and 3 were identified as $1-O-(9Z,12Z,15Z-octatrienoyl)-2-O-(9Z,12Z,15Z-octatrienoyl)-3-O-{\beta}-D-galactopyranosyl$ glycerol, $2(R)-1-O-octadecanoyl-2-O-(9Z,12Z,15Z-octatrienoyl)-3-O-{\beta}-D-galactopyranosyl$ glycerol, and $3{\beta}-hydroxylup-20(29)-ene$ acetate, respectively. Compound 2 (1mg/kg p.o.) has approximately 16% hypoglycemic activity in streptozotocin-induced diabetic rats.
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