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Saccharomyces cerevisiae 의 회분식 배양에서 세라마이드의 생산
김세경,노용호,윤현식 한국생물공학회 2008 KSBB Journal Vol.23 No.5
Ceramide has become a widely used ingredient in cosmetic and pharmaceutical industries, however, only a few yeast strains were investigated for the synthesis of ceramide and the concentration was very low. Ceramide is not only a core intermediate of sphingolipids but also an important modulator of many cellular events including apoptosis, cell cycle arrest, senescence, differentiation, and stress responses. In this study S.cerevisiae was grown in a batch culture and the cellular content of ceramide was measured at different growth phases. The ceramide content was highest at stationary phase and 2.01 mg ceramide/g cell was obtained.
Effects of Expression of lcb1/lcb2 and lac1/lag1 Genes on the Biosynthesis of Ceramides
김세경,Yong Ho Noh,Ja-Ryong Koo,윤현식,Nancy A. DaSilva 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.1
Ceramides are important signal messenger molecules due to their role in regulating diverse responses such as cell cycle arrest, apoptosis, and senescence. Yeasts are more suitable for ceramides production than any other microorganisms since they grow fast and are non-pathogenic. However, regulations of the expression of genes involved in sphingolipid synthesis pathway are required to enhance ceramides production. In this study, we investigated the effects of co-expression of two pairs of genes that encode for serine palmitoyltransferase and ceramide synthase,respectively. Effect of other genes of the enzymes associated with the sphingolipid synthesis pathway, 3-ketosphinganine reductase and sphinganine C-4 hydroxylase were also studied and compared. The genes were cloned in to pESC-URA vector. Saccharomyces cerevisiae was cultivated aerobically in YPDG medium at 30oC. Ceramides were seperated from cell extracts by solvent extraction and quantified by HPLC with ELSD. The highest ceramides production (10.52 mg ceramides/g cell) was obtained when 3-ketosphinganine reductase, which is encoded by tsc10gene, was overexpressed. Also, S. cerevisiae SCEL2,1 overexpressing serine palmitoyltransferase encoded by lcb2 and lcb1 genes, and S. cerevisiae SCEG1C1 overexpressing ceramide synthase encode by lag1 and lac1 genes, showed a high level of ceramides production (10.08 mg ceramides/g cell and 9.88 mg ceramides/g cell, respectively).
金世景 최신의학사 1971 最新醫學 Vol.14 No.7
In spite of many available antibiotics and chemotherapeutics, genitourinary tract infection still remaines as troublesome subject for urologists. New bactericidal agent, Bactrim(trimethoprim-sulfamethoxazole) was administered in 60 cases of genitourinary tract infection which were resistant to many other antibiotics and following results were obtained. 1) Among 22 cases of gonococcal urethritis, 20 cases were cured rapidly, 2 cases were improved. 2) Among 20 cases of non-gonococcal urethritis, 15 cases were cured and 5 cases were improved. 3) Among 9 cases of chronic prostatitis, one case was cured and mild improvement were noted in remaining 8 cases. 4) 8 cases of pyelonephritis showed rapid improvement in both clinically and bacteriologically.