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RISS 인기검색어
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강동진,石井龍一,이인중 慶北大學校 農業科學技術硏究所 2003 慶北大農學誌 Vol.21 No.-
Plants are often subjected to periods of soil and atmospheric water deficit during their life cycle. To find critical levels for identification of tolerant rice variety to salt- and drought-stresses, we investigated the water deficiency in the leaf of a Dongjinbyeo (DJ) cultivar, identified as intolerant variety, subjected to NaCl- and Polyethylene glycol 6000 (PEG)- treatments. The relative water content and water potential in leaf of DJ plant sharply declined along the high concentration and time after treatment in NaCl- and PEG-treated rice plants. To elucidate the method of simple screening of tolerant variety to salt- and drought-stresses, we examined the relationship between relative water content and water potential of leaves in NaCl- and PEG-treated rice plants. The relationship between relative water content and water potential in leaf of DJ plant showed the highest correlation in 80 mM NaCl-treatment, and showed high correlation only 8% PEG treatment. These results indicate that the critical level of salt stress for screening of tolerant rice was 80 mM NaCl at 48 h after NaCl treatment, and the critical concentration of drought stress for screening of tolerant rice was 8% PEG at 96 h after PEG treatment.
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Agar 배지를 이용한 건조 및 염 처리에 대한 벼 식물체의 근계 변화
강동진,석정용일,김길웅,이인중 한국생명과학회 2004 생명과학회지 Vol.14 No.3
본 실험은 고체배지를 이용한 새로운 실험방법을 이용하여 건조 및 염 스트레스에 대한 식물체의 형태학적인 특성을 뿌리신장률, 근단구조, 물질생산 등의 측면에서 조사하였다. PEG 및 NaCl 처리조건에서 처리 농도가 증가할수록 벼 식물체의 뿌리신장이 현저하게 저하되었으며, PEC 및 NaCl 처리에 따른 벼 식물체의 뿌리는 methyl-lignin 축적에 의한 리그닌화가 진행되었으며, 수분결핍을 극복하려는 기작으로 표피세포를 변형시킨 근모의 발생이 관찰되었다. 또한 PEG 및 NaCl 처리에 의한 벼 식물체의 지상부와 지하부의 건물 생산량의 현저한 감소는 지상부보다 지하부에서 뚜렷하였으며, 그 결과 PEG 및 NaCl 처 처리농도를 증가시킴에 따라 TR율의 증가를 보였다. 상기의 결과로부터 건조 및 염 처리를 한 고체배지를 이용함으로써 벼의 경우 스트레스 처리 전 약 2주간 유묘를 생육시키는 양액재배에 비해 발아기의 각종 스트레스에 대한 식물체 반응의 관찰과 내성 특성 검정에 유용할 것으로 사료된다. This study was investigated the changes of root length, tissue structure of root tip, and dry matter production of a Dongjinbyeo (DJ) cultivar subjected to 0.4 % agar medium with various concentration of NaCl (salt stress)- and polyethylene glycol 6000 (PEG, drought stress). Root length and dry weight of DJ plant were declined along the high concentration of PEG and NaCl in rice plants. To elucidate the changes of tissue structure in root tip to PEG- and NaCl-treatments, we examined the microscopic observation of root tip in NaCl- and PEG-treated rice plants using Toluidine blue O. By Toluidine blue O staining, methyl-lignin accumulation was found in the epidermis and outer cortex of the elongation zone at an early stage of PEG treatment, whereas was found only the outer cortex of the elongation zone of NaCl-treated root tip. The epidermis of NaCl-treated root tip became soften instead of methyl-lignin accumulation. TR ratio was increased along the high concentration in PEG- and NaCl-treated rice plant as a result of inhibited root elongation under PEG- and NaCl-treatment. From these morphological changes in root stimulated by drought and salt stress, we suggest that agar medium is useful to identify tolerant variety in germination stage under stressful environments.
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裵成國,許益,石井龍一,玖村敦彦 韓國作物學會 1985 Korean journal of crop science Vol.30 No.2
이식후 생육초기의 잎담배 (품종 :Bulgaria)와 3연근 인삼을 공시하여 광강도를 580, 1,280, 1,770, 2,580 u E/m2 /sec로 각각 3,6,9시간을 엽온 22℃ 의 조건에서 처리한 후 CO2 농도 320ppm에서 광일광합성속도를 조사하였다. 1. 처리전 인삼의 광포화점은 어느 온도 조건에서나 공히 550u E/m2 /sec 정도였고, 광합성속도는 20℃ 에서 7.2, 35℃ 에서 5.2mg.CO2 /dm2 /hr이었다. 잎담배는 광포화점이 25℃ 에서 1,600u E/m2 /sec에서 나타났고, 광합성속도는 25℃ 에서 14.3, 15℃ 에서는 10.3mg.CO2 /dm2 /hr이었다. 2. 잎담배는 어느 처리에서나 광저해가 일어나지 않았다. 3. 인삼은 3시간 처리에서는 2,580u E/m2 /sec의 광도에서만 광합성속도가 11.4% 저하되었고, 9시간 처리에서는 1,270u E/m2 /sec 광도에서 8.5%, 1,770u E/m2 /sec에서 9.5%, 2,580u E/m2 /sec에서 20.3%로 각각 저하되어 광강도가 크고 조사기간이 길수록 광저해가 컸다. 4. 인삼에서 기공저항은 처리간에 차이가 없었으나, 엽육저항은 광저해를 받은 처리에서 크게 나타냈고. 담배에서는 처리간에 차이가 인정되지 않았다. Photoinhibition studies were conducted with ginseng (Panax ginseng) grown under shade and tobacco (N. tobaccum cv. Bulgaria) grown under full sunlight. The plants were exposed to light intensity of 580, 1280, 1770, 2580 ~mu E/㎡/sec in normal air for 3.6 9 hours. Light saturation of ginseng was observed at 550 ~mu E/㎡/sec and that of tobacco was at 1600 ~mu E/㎡/sec. Tobacco exposed to high light intensity and long duration of light irradiation didn't show entire reduction in photosynthetic capacity. But in ginseng, 20.3% of photosynthesis was reduced in light intensity of 2,580 ~mu E/㎡/sec during 9 hours. Light response in photosynthesis differed considerably between tobacco and ginseng, and ginseng exposed to high light intensity showed remarkable reduction in photosynthesis. The extent of photoinhibition of ginseng was dependent on the length of exposure to the high light intensity. Stomatal resistance of ginseng seemed not to be changed by photoinhibition but mesophyll resistance was increased.
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